CAJ | 학술논문

目的研究大蒜素(diallyl thiosulfinate, allicin)对链脲佐菌素(streptozotocin,STZ)所致糖尿病大鼠胰岛的保护作用.方法实验用SD大鼠经高脂高糖饲料喂养8周后,通过腹腔注射STZ的方法诱导糖尿病大鼠模型,选取100只随机分为:糖尿病模型组、大蒜素(5、10和20 mg/kg)治疗组和盐酸二甲双胍(200 mg/kg)治疗组,每组20只,并另设正常对照组.大蒜素各治疗组通过腹腔注射给药,盐酸二甲双胍治疗组灌胃给药,正常对照组和糖尿病模型对照组腹腔注射给予等体积的生理盐水,每天1次,疗程4周. 分别于给药前和给药后每周测定各组大鼠空腹血糖和胰岛素水平;4周后,通过HE染色观察胰腺组织病变;通过TUNEL染色观察胰岛细胞凋亡并计算凋亡指数(apoptosis index, AI);测定血清中总抗氧化能力(T-AOC)水平和丙二醛(MDA)含量;测定胰腺组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)活性. 结果与糖尿病模型组比较,大蒜素治疗组大鼠胰腺组织病变程度和胰岛细胞凋亡状况明显得到改善,其中大蒜素10、20 mg/kg治疗组大鼠胰岛细胞AI指数明显降低(P<0.01)、大鼠空腹血糖水平显著降低、胰岛素水平显著升高(P<0.05,P<0.01)、血清中MDA含量显著降低、胰腺组织中SOD、CAT活性显著升高(P<0.05,P<0.01),大蒜素20 mg/kg治疗组大鼠血清中T-AOC水平显著升高、胰腺组织中GSH-PX活性显著升高(P<0.05);大蒜素20 mg/kg治疗组,较盐酸二甲双胍治疗组大鼠胰岛素水平显著升高、血清中T-AOC水平显著升高、胰腺组织中SOD、GSH-Px和CAT活性显著升高(P<0.05,P<0.01). 结论大蒜素能有效提高胰岛素分泌、降低血糖、改善胰腺组织病变并抑制胰岛细胞凋亡,提示大蒜素对STZ素所致糖尿病大鼠胰岛具有剂量依赖性的保护作用,其作用机制可能与大蒜素能有效改善抗氧化酶活性、提高自由基清除能力、抑制氧化应激损伤有关. 目的研究大蒜素(diallyl thiosulfinate, allicin)對鏈脲佐菌素(streptozotocin,STZ)所緻糖尿病大鼠胰島的保護作用.方法實驗用SD大鼠經高脂高糖飼料餵養8週後,通過腹腔註射STZ的方法誘導糖尿病大鼠模型,選取100隻隨機分為:糖尿病模型組、大蒜素(5、10和20 mg/kg)治療組和鹽痠二甲雙胍(200 mg/kg)治療組,每組20隻,併另設正常對照組.大蒜素各治療組通過腹腔註射給藥,鹽痠二甲雙胍治療組灌胃給藥,正常對照組和糖尿病模型對照組腹腔註射給予等體積的生理鹽水,每天1次,療程4週. 分彆于給藥前和給藥後每週測定各組大鼠空腹血糖和胰島素水平;4週後,通過HE染色觀察胰腺組織病變;通過TUNEL染色觀察胰島細胞凋亡併計算凋亡指數(apoptosis index, AI);測定血清中總抗氧化能力(T-AOC)水平和丙二醛(MDA)含量;測定胰腺組織中超氧化物歧化酶(SOD)、穀胱甘肽過氧化物酶(GSH-PX)、過氧化氫酶(CAT)活性. 結果與糖尿病模型組比較,大蒜素治療組大鼠胰腺組織病變程度和胰島細胞凋亡狀況明顯得到改善,其中大蒜素10、20 mg/kg治療組大鼠胰島細胞AI指數明顯降低(P<0.01)、大鼠空腹血糖水平顯著降低、胰島素水平顯著升高(P<0.05,P<0.01)、血清中MDA含量顯著降低、胰腺組織中SOD、CAT活性顯著升高(P<0.05,P<0.01),大蒜素20 mg/kg治療組大鼠血清中T-AOC水平顯著升高、胰腺組織中GSH-PX活性顯著升高(P<0.05);大蒜素20 mg/kg治療組,較鹽痠二甲雙胍治療組大鼠胰島素水平顯著升高、血清中T-AOC水平顯著升高、胰腺組織中SOD、GSH-Px和CAT活性顯著升高(P<0.05,P<0.01). 結論大蒜素能有效提高胰島素分泌、降低血糖、改善胰腺組織病變併抑製胰島細胞凋亡,提示大蒜素對STZ素所緻糖尿病大鼠胰島具有劑量依賴性的保護作用,其作用機製可能與大蒜素能有效改善抗氧化酶活性、提高自由基清除能力、抑製氧化應激損傷有關.
목적 연구대산소(diallyl thiosulfinate, allicin)대련뇨좌균소(streptozotocin,STZ)소치당뇨병대서이도적보호작용.방법 실험용SD대서경고지고당사료위양8주후,통과복강주사STZ적방법유도당뇨병대서모형,선취100지수궤분위:당뇨병모형조、대산소(5、10화20 mg/kg)치료조화염산이갑쌍고(200 mg/kg)치료조,매조20지,병령설정상대조조.대산소각치료조통과복강주사급약,염산이갑쌍고치료조관위급약,정상대조조화당뇨병모형대조조복강주사급여등체적적생리염수,매천1차,료정4주. 분별우급약전화급약후매주측정각조대서공복혈당화이도소수평;4주후,통과HE염색관찰이선조직병변;통과TUNEL염색관찰이도세포조망병계산조망지수(apoptosis index, AI);측정혈청중총항양화능력(T-AOC)수평화병이철(MDA)함량;측정이선조직중초양화물기화매(SOD)、곡광감태과양화물매(GSH-PX)、과양화경매(CAT)활성. 결과 여당뇨병모형조비교,대산소치료조대서이선조직병변정도화이도세포조망상황명현득도개선,기중대산소10、20 mg/kg치료조대서이도세포AI지수명현강저(P<0.01)、대서공복혈당수평현저강저、이도소수평현저승고(P<0.05,P<0.01)、혈청중MDA함량현저강저、이선조직중SOD、CAT활성현저승고(P<0.05,P<0.01),대산소20 mg/kg치료조대서혈청중T-AOC수평현저승고、이선조직중GSH-PX활성현저승고(P<0.05);대산소20 mg/kg치료조,교염산이갑쌍고치료조대서이도소수평현저승고、혈청중T-AOC수평현저승고、이선조직중SOD、GSH-Px화CAT활성현저승고(P<0.05,P<0.01). 결론 대산소능유효제고이도소분비、강저혈당、개선이선조직병변병억제이도세포조망,제시대산소대STZ소소치당뇨병대서이도구유제량의뢰성적보호작용,기작용궤제가능여대산소능유효개선항양화매활성、제고자유기청제능력、억제양화응격손상유관.
Objective To investigate the prote ctive effects of allicin (diallyl thiosulfinate) on islet tissue in diabetic rats induced by streptozotocin (STZ). Methods A hundred diabetic rat models induced by intraperitoneal injecting STZ were randomly devided into five groups:diabetic model control group, allicin(5,10 and 20 mg/kg) treated groups and metformin hydrochloride 200 mg/kg treated group. Another 20 same-aged rats were used as normal group. Each week before and after the drug was given, the levels of blood sugar and insulin were determined. Four weeks later, the histopathological changes in pancreatic tissue and islet cells apoptosis were observed, and the apoptosis index (AI) was analyzed; the level of T-AOC and the content of MDA in serum were determined; the activities of SOD, GSH-Px, CAT in pancreatic tissue were also determined. Results Compared with the diabetic model control group, the levels of insulin in allicin 10, 20 mg/kg treated groups were significantly increased and the blood sugar level was significantly decreased (P<0.05, P<0.01); the pancreatic tissue histopathological changes and islet cells apoptosis were obviously improved, the AI was significantly decreased (P<0.01); the content of MDA in serum was significantly decreased; the activities of SOD and CAT in pancreatic tissue were significantly increased(P<0.05, P<0.01). The level of T-AOC in allicin 20 mg/kg treated group was significantly increased (P<0.05), and the activity of GSH-PX in pancreatic tissue was significantly increased (P<0.05). Compared with Metformin hydrochloride 200 mg/kg treated group, the level of insulin in allicin 20 mg/kg treated groups was significantly increased (P<0.05), the level of T-AOC in serum was significantly increased (P<0.05), the activity of SOD, GSH-Px,CAT in pancreatic tissue were also significantly increased (P<0.05, P<0.01). Conclusion Allicin could effectively improve insulin secretion, lower blood sugar, depress the pancreatic tissue histopathological changes and islet cells apoptosis, suggesting that allicin has dose-dependent protective effects on islet tissue in diabetic rats induced by STZ, which is perhaps related to its effects of improving antioxidant ability and inhibiting the oxidative stress.