CAJ | 학술논문

目的：研究豚鼠过敏性猝死后血清IgE随死亡时间（PMI）的变化规律，探讨冷藏尸体血清IgE在过敏性猝死法医学鉴定中的价值。方法建立过敏性猝死的豚鼠模型，置室温（20℃）保存6 h后，于4℃冷藏，于死后6、12、24、36和48 h取血清，同时设立对照组。采用酶联免疫吸附试验检测豚鼠血清IgE的含量。结果过敏性猝死豚鼠与对照组豚鼠血清IgE含量相比较，差异有统计学意义（P<0.05）；死后6、12、24、36和48 h之间血清IgE含量相比较，差异无统计学意义（P>0.05）。结论过敏性猝死6 h后将尸体置于4℃冷藏条件下保存，48 h内进行血清IgE检测对过敏性猝死的法医学鉴定仍具有参考价值。
목적：연구돈서과민성졸사후혈청IgE수사망시간（PMI）적변화규률，탐토랭장시체혈청IgE재과민성졸사법의학감정중적개치。방법건립과민성졸사적돈서모형，치실온（20℃）보존6 h후，우4℃랭장，우사후6、12、24、36화48 h취혈청，동시설립대조조。채용매련면역흡부시험검측돈서혈청IgE적함량。결과과민성졸사돈서여대조조돈서혈청IgE함량상비교，차이유통계학의의（P<0.05）；사후6、12、24、36화48 h지간혈청IgE함량상비교，차이무통계학의의（P>0.05）。결론과민성졸사6 h후장시체치우4℃랭장조건하보존，48 h내진행혈청IgE검측대과민성졸사적법의학감정잉구유삼고개치。
Objective T o investigate the serum IgE w ith various postm ortem intervals (PMI) in guinea pigs due to sudden death from anaphylactic shock and to explore the effect of refrigeration of corpse on serum IgE level and its application value in forensic m edicine. Methods T he anim al death m odels of anaphylactic shock w ere established. T he corpses w ere preserved at room tem perature (20℃) for 6 h and then refrigerated at 4℃. T he serum w as sam pled at 6, 12, 24, 36 and 48 hours after death. T he IgE level of serum w as detected w ith ELISA . The control group w as also established. Results The serum IgE level had significant difference betw een the experim ental group and the control group (P<0.05). T here w as no significant difference am ong the experim ental groups at 6, 12, 24, 36 and 48 hours post-m ortem (P>0.05). Conclusion If the corpses w ere placed in 4℃ conditions 6 hours after anaphylactic death, the serum IgE still show s a good m arker w ithin 48 h for forensic investigation.