CAJ | 학술논문

目的：通过real-time PCR技术检测大鼠肌肉挫伤后组织中分泌型卷曲相关蛋白5（secreted frizzled-related protein 5，SFRP5） mRNA的表达变化情况，分析其与挫伤时间及损伤类型的关系。方法90只SD大鼠，随机分为肌肉挫伤后4、8、12、16、20、24、28、32、36、40、44、48 h组，切创后4、8 h组和对照组。于相应时间点取损伤处肌肉组织样本。提取组织中总RNA，反转录后用于SFRP5 mRNA表达量的检测。结果SFRP5 mRNA的表达量在损伤后很快明显下降，在20 h时降至最低，然后逐渐上升，但挫伤后48 h内其表达量均低于对照组；切创组SFRP5 mRNA的表达量均明显降低，在伤后4 h，切创组低于挫伤组，而在8 h时两组的表达差异无统计学意义。结论 SFRP5 mRNA在大鼠肌肉挫伤后一段时间内呈现规律性表达，可望作为推断骨骼肌损伤时间的指标之一。
목적：통과real-time PCR기술검측대서기육좌상후조직중분비형권곡상관단백5（secreted frizzled-related protein 5，SFRP5） mRNA적표체변화정황，분석기여좌상시간급손상류형적관계。방법90지SD대서，수궤분위기육좌상후4、8、12、16、20、24、28、32、36、40、44、48 h조，절창후4、8 h조화대조조。우상응시간점취손상처기육조직양본。제취조직중총RNA，반전록후용우SFRP5 mRNA표체량적검측。결과SFRP5 mRNA적표체량재손상후흔쾌명현하강，재20 h시강지최저，연후축점상승，단좌상후48 h내기표체량균저우대조조；절창조SFRP5 mRNA적표체량균명현강저，재상후4 h，절창조저우좌상조，이재8 h시량조적표체차이무통계학의의。결론 SFRP5 mRNA재대서기육좌상후일단시간내정현규률성표체，가망작위추단골격기손상시간적지표지일。
Objective T o investigate the relationship betw een the expression of secreted frizzled-related protein 5 (SFR P5) m RNA and the tim e interval after skeletal m uscle injury in rats by real-tim e PC R . Methods A total of ninety SD rats w ere random ly divided into the contusion groups at different tim es including 4 h, 8 h, 12 h, 16 h, 20 h, 24 h, 28 h, 32 h, 36 h, 40 h, 44 h, 48 h after contusion, incision groups at different tim es including 4h and 8h after incision and the control group. T he sam ples w ere taken from the contused zone at different tim e points. T he total RNA w as isolated from the sam ples and re-versely transcribed to analyze the expression levels of SFRP5 m RNA . Results C om pared to the control group, the expression of SFRP5 m RNA in contusion groups w ere dow n-regulated w ithin 48 h after con-tusion and reached the low est level at 20 h, and the expression of SFRP5 m RNA gradually increased from 20 h to 48 h after contusion. T he expression of SFRP5 m RNA in the incised groups w ere signifi-cantly low er than that of the contusion groups at 4 h after injury. A t the tim e of 8 h, the expression levels betw een the contusion and incision groups show ed no statistically significant difference. Conclusion It is suggested that SFRP5 m RNA analysis m ay show regular expression and can be a m arker for estim ation of skeletal m uscle injury age.