国际药学研究杂志
國際藥學研究雜誌
국제약학연구잡지
Journal of International Pharmaceutical Research
2015年
5期
646-649
,共4页
高效液相色谱法%SN38-PA%SN38%含量测定
高效液相色譜法%SN38-PA%SN38%含量測定
고효액상색보법%SN38-PA%SN38%함량측정
HPLC%SN38-PA%SN38%quantitative analysis
目的 建立同时测定大鼠血浆中7-乙基-10-羟基喜树碱-10-棕榈酸酯(SN38-PA)及其活性代谢产物7-乙基-10-羟基喜树碱(SN38)含量的高效液相色谱法. 方法 以10-羟基喜树碱(10-CPT)作为内标,甲酸酸化血浆,甲醇沉淀蛋白. 采用Agilent C18柱(4.6 mm×250 mm,5μm)色谱柱,以甲醇-0.2%甲酸水溶液为流动相进行梯度洗脱,流速1 ml/min,紫外检测波长372 nm,柱温:30℃. 结果 大鼠血浆中SN38-PA和SN38线性范围分别为0.25~62.5(r=0.9998)和0.05~12.5μg/ml(r=0.9997),最低定量限分别为0.18和0.04μg/ml; 两组分平均绝对回收率分别为95.89%和97.03%; 平均相对回收率分别为99.54%和99.84%.2组分日内、日间精密度的RSD%均<3%.结论 本分析方法快速、简便、准确度和灵敏度高,可用于SN38-PA及其活性代谢产物SN38的体内检测.
目的 建立同時測定大鼠血漿中7-乙基-10-羥基喜樹堿-10-棕櫚痠酯(SN38-PA)及其活性代謝產物7-乙基-10-羥基喜樹堿(SN38)含量的高效液相色譜法. 方法 以10-羥基喜樹堿(10-CPT)作為內標,甲痠痠化血漿,甲醇沉澱蛋白. 採用Agilent C18柱(4.6 mm×250 mm,5μm)色譜柱,以甲醇-0.2%甲痠水溶液為流動相進行梯度洗脫,流速1 ml/min,紫外檢測波長372 nm,柱溫:30℃. 結果 大鼠血漿中SN38-PA和SN38線性範圍分彆為0.25~62.5(r=0.9998)和0.05~12.5μg/ml(r=0.9997),最低定量限分彆為0.18和0.04μg/ml; 兩組分平均絕對迴收率分彆為95.89%和97.03%; 平均相對迴收率分彆為99.54%和99.84%.2組分日內、日間精密度的RSD%均<3%.結論 本分析方法快速、簡便、準確度和靈敏度高,可用于SN38-PA及其活性代謝產物SN38的體內檢測.
목적 건립동시측정대서혈장중7-을기-10-간기희수감-10-종려산지(SN38-PA)급기활성대사산물7-을기-10-간기희수감(SN38)함량적고효액상색보법. 방법 이10-간기희수감(10-CPT)작위내표,갑산산화혈장,갑순침정단백. 채용Agilent C18주(4.6 mm×250 mm,5μm)색보주,이갑순-0.2%갑산수용액위류동상진행제도세탈,류속1 ml/min,자외검측파장372 nm,주온:30℃. 결과 대서혈장중SN38-PA화SN38선성범위분별위0.25~62.5(r=0.9998)화0.05~12.5μg/ml(r=0.9997),최저정량한분별위0.18화0.04μg/ml; 량조분평균절대회수솔분별위95.89%화97.03%; 평균상대회수솔분별위99.54%화99.84%.2조분일내、일간정밀도적RSD%균<3%.결론 본분석방법쾌속、간편、준학도화령민도고,가용우SN38-PA급기활성대사산물SN38적체내검측.
Objective To develop an HPLC method for the simultaneous determination of 7-ethyl-10-hydroxycamptothecin-10-palmitic acid ester(SN38-PA)and its active metabolite 7-ethyl-10-hydroxycamptothecin(SN38)in rat plasma. Methods The inter standard was 10-hydroxycamptothecin. The protein in plasma was precipitated with methanol after acidification with formic acid. SN38-PA and SN38 were separated on Agilent C18 column(4.6 mm×250 mm,5μm) with gradient elution by using the mobile phase of methanol-0.2% formic acid solution. The flow rate was 1 ml/min. The detection wavelength was set at 372 nm. The column temperature was maintained at 30℃. Results The linear ranges for SN38-PA and SN38 were 0.25-62.5(r=0.9998) and 0.05-12.5 μg/ml (r=0.9997) respectively. The limits of quantification were 0.18 and 0.04 μg/ml, respectively. The average relative recovery of SN38-PA and SN38 were 95.89% and 97.03%. The average absolutely recovery of SN38-PA and SN38 were 99.54% and 99.84%. The RSD for intra-day and inter-day were both less than 3%. Conclusion The method is fast, convenient, accurate and sensitive, so it can be used for determination of SN38-PA and SN38 in vivo.
