CAJ | 학술논문

目的：研究头顶一颗珠( TTM)注射液对肝癌模型小鼠免疫功能的影响。方法建立H22小鼠肝癌模型,将造模成功的肝癌小鼠依据随机数字表法分为模型组、氟尿嘧啶(5-FU)组、5-FU+TTM组和TTM组,每组10只。模型组、5-FU组每日按10 mg/kg腹腔注射生理盐水及5-FU,5-FU+TTM组在注射5-FU的同时每日腹腔按7．2 g/kg注射TTM,TTM组按7．2 g/kg注射TTM。给药5 d后测定各组胸腺、脾脏指数,检测外周血中白细胞、淋巴细胞及CD3、CD4、CD8细胞的百分比,血清中白细胞介素2( IL-2)、肿瘤坏死因子α( TNF-α)水平。结果5-FU组和TTM组的胸腺指数与模型组比较差异无统计学意义(P>0．05),而5-FU+TTM组显著高于模型组(P<0．05)。与模型组比较,5-FU 组外周血白细胞与淋巴细胞数量明显减少( P <0．05), TTM组显著增加( P <0．05)；5-FU+TTM组白细胞数与模型组比较差异无统计学意义(P>0．05)；与5-FU组比较,TTM组白细胞与淋巴细胞数量显著增加(P<0．05),5-FU+TTM组白细胞数量明显增多(P<0．05),但淋巴细胞数量变化差异无统计学意义(P>0．05)。相对于模型组,TTM组、5-FU+TTM组及5-FU组CD3、CD4百分比显著增加(P<0．05),CD8百分比变化差异无统计学意义(P>0．05)；与较5-FU+TTM组比较,TTM组、5-FU组CD3、CD4百分比差异无统计学意义,但CD8百分比显著增加( P<0．05)。相对于模型组,TTM组、5-FU+TTM组及5-FU组IL-2、TNF-α水平显著增加( P<0．05)；与TTM组比较,5-FU+TTM组、5-FU组 IL-2水平差异无统计学意义( P >0．05),但5-FU 组 TNF-α水平显著增加( P <0．05),5-FU+TTM组差异则无统计学意义(P>0．05)。结论 TTM可以促进T细胞的成熟与迁移,提高肝癌小鼠IL-2、TNF-α水平,增强机体免疫力,具有抗肿瘤功能。
목적：연구두정일과주( TTM)주사액대간암모형소서면역공능적영향。방법건립H22소서간암모형,장조모성공적간암소서의거수궤수자표법분위모형조、불뇨밀정(5-FU)조、5-FU+TTM조화TTM조,매조10지。모형조、5-FU조매일안10 mg/kg복강주사생리염수급5-FU,5-FU+TTM조재주사5-FU적동시매일복강안7．2 g/kg주사TTM,TTM조안7．2 g/kg주사TTM。급약5 d후측정각조흉선、비장지수,검측외주혈중백세포、림파세포급CD3、CD4、CD8세포적백분비,혈청중백세포개소2( IL-2)、종류배사인자α( TNF-α)수평。결과5-FU조화TTM조적흉선지수여모형조비교차이무통계학의의(P>0．05),이5-FU+TTM조현저고우모형조(P<0．05)。여모형조비교,5-FU 조외주혈백세포여림파세포수량명현감소( P <0．05), TTM조현저증가( P <0．05)；5-FU+TTM조백세포수여모형조비교차이무통계학의의(P>0．05)；여5-FU조비교,TTM조백세포여림파세포수량현저증가(P<0．05),5-FU+TTM조백세포수량명현증다(P<0．05),단림파세포수량변화차이무통계학의의(P>0．05)。상대우모형조,TTM조、5-FU+TTM조급5-FU조CD3、CD4백분비현저증가(P<0．05),CD8백분비변화차이무통계학의의(P>0．05)；여교5-FU+TTM조비교,TTM조、5-FU조CD3、CD4백분비차이무통계학의의,단CD8백분비현저증가( P<0．05)。상대우모형조,TTM조、5-FU+TTM조급5-FU조IL-2、TNF-α수평현저증가( P<0．05)；여TTM조비교,5-FU+TTM조、5-FU조 IL-2수평차이무통계학의의( P >0．05),단5-FU 조 TNF-α수평현저증가( P <0．05),5-FU+TTM조차이칙무통계학의의(P>0．05)。결론 TTM가이촉진T세포적성숙여천이,제고간암소서IL-2、TNF-α수평,증강궤체면역력,구유항종류공능。
Objective To study the effect of Trillium tschonoskii Maxim( TTM) injection on immune functions of mice modeled with hepatoma. Methods H22 hepatoma-mice model was built, and the hepa-toma-mice with successful modeling were randomly divided into model group,5-FU group,5-FU+TTM group and TTM group,10 mice in each group. The model group mice and the 5-FU group mice were given physio-logical saline intraperitoneal injection with 10 mg/kg per day. The 5-FU+TTM group mice were given TTM intraperitoneal injection with 7. 2 g/kg at the same time, and the TTM group mice were injected with 7. 2 g/kg. The index of thymus and spleen,the proportion of leucocyte,lymphocyte and CD3 ,CD4 ,CD8 cells in the periphearl blood,and the concentration of IL-2,TNF-αin serum after five days′medicines were meas-ured. Results The thymus index of mice in 5-FU group and TTM group had no statistical significance and 5-FU+TTM group increased obviously compared with the model group(P<0. 05);the amount of leucocyte and lymphocyte of peripheral blood in 5-FU group decreased obviously ( P <0. 05 ) and the TTM group increased obviously(P <0. 05)compared with the model group;the amount of leucocyte in 5-FU +TTM group had no statistical significance compared with the model group;the amount of leucocyte and lymphocyte in TTM group increased obviously compared with 5-FU group(P<0. 05);the amount of leucocyte increased obviously in 5-FU+TTM group,but the amount of lymphocyte had no statistical significance. The proportion of CD3,CD4 in TTM group,5-FU+TTM group and 5-FU group increased significantly(P<0. 05) and the proportion of CD8 had no statistical significance compared with the model group(P>0. 05). The proportion of CD3 ,CD4 in TTM group,5-FU group had no statistical significance,but the proportion of CD8 increased obviously compared with 5-FU+TTM group(P<0. 05). The contents of IL-2,TNF-αin TTM group,5-FU+TTM group and 5-FU group increased obviously compared with the model group(P<0. 05);compared with TTM group,the contents of IL-2 had no obvious difference and had no statistical significance (P>0. 05) in 5-FU+TTM group and 5-FU group,but contents of TNF-α in 5-FU group increased obviously(P<0. 05) and the contents in 5-FU+TTM group had no statistical significance ( P >0. 05 ) . Conclusion TTM can advance the maturation and metastasis of T cells,improve the level of IL-2,TNF-α of mice with hepatoma, strengthen the organic immunity,thus has the effect of anti-tumor.