CAJ | 학술논문

为比较酸奶中分离的短乳杆菌和鼠李糖乳杆菌对消化道肿瘤的潜在抑制效果，在肿瘤模型建立前2周给小鼠分别灌胃2株乳酸菌（2×108cfu），此后建立鼠结肠癌模型以比较2株乳酸菌对其是否具有抑制作用。通过给小鼠皮下注射CT26．WT细胞获得皮下增生物，经病理组织学检测为肿瘤后，通过腹部手术移植皮下瘤块至肠道表面，进而建立鼠结肠癌肿瘤模型。模型建立后通过腹部B超扫描监测肿瘤体积，并于模型建立2周后处死小鼠、分离肿瘤称重，计算抑瘤率。结果表明：皮下注射CT26．WT可以诱导获得皮下肿瘤，腹部手术、OB胶黏附瘤块建立了鼠结肠癌皮下移植瘤模型，荷瘤率为100％，术后存活率为96％以上。模型建立7 d后，腹部B超扫描检测结果表明，灌胃短乳杆菌组荷瘤鼠肿瘤的体积显著低于PBS组（ P＜0?05），而鼠李糖乳杆菌灌胃组荷瘤鼠肿瘤体积也有下降趋势，但与PBS组相比差异不显著。分离荷瘤鼠肿瘤组织，灌胃2株乳酸菌组小鼠肿瘤重均有所下降，且短乳杆菌组更为明显，短乳杆菌抑瘤率为36?47％，鼠李糖乳杆菌抑瘤率为21?68％。构建了鼠结肠癌原位移植瘤模型，且短乳杆菌对鼠结肠癌具有较好的抑制效果，优于鼠李糖乳杆菌。
위비교산내중분리적단유간균화서리당유간균대소화도종류적잠재억제효과，재종류모형건립전2주급소서분별관위2주유산균（2×108cfu），차후건립서결장암모형이비교2주유산균대기시부구유억제작용。통과급소서피하주사CT26．WT세포획득피하증생물，경병리조직학검측위종류후，통과복부수술이식피하류괴지장도표면，진이건립서결장암종류모형。모형건립후통과복부B초소묘감측종류체적，병우모형건립2주후처사소서、분리종류칭중，계산억류솔。결과표명：피하주사CT26．WT가이유도획득피하종류，복부수술、OB효점부류괴건립료서결장암피하이식류모형，하류솔위100％，술후존활솔위96％이상。모형건립7 d후，복부B초소묘검측결과표명，관위단유간균조하류서종류적체적현저저우PBS조（ P＜0?05），이서리당유간균관위조하류서종류체적야유하강추세，단여PBS조상비차이불현저。분리하류서종류조직，관위2주유산균조소서종류중균유소하강，차단유간균조경위명현，단유간균억류솔위36?47％，서리당유간균억류솔위21?68％。구건료서결장암원위이식류모형，차단유간균대서결장암구유교호적억제효과，우우서리당유간균。
To compare antitumor effect of Lactobacillus brevis and Lactobacillus rhamnosus strains on gastrointestinal tumor, colon cancer model was constructed after the mice were drenched by these two strains for continuously two weeks as 2 × 108 cfu. CT26. WT cells ( cell viability>95%) were slowly injected subcutaneously into the mice to obtain xenograft. Once the subcutaneous xenograft was determined as tumor tissue by histopathology, one intact tumor block was implanted onto the se?rosa of the cecum through abdominal operation in order to construct mouse colon caner model. Then, tumor volume was determined by abdominal B ultrasound and the tumor was weighted to count tumor inhibitory . The result showed that colon cancer model was constructed and tumor bearing rate was up to 100%, and postoperative survival rate was up to 96%. After 7 d of post?implantation, a significant decrease in tumor volume was observed in Lactobacillus brevis group compared to PBS group ( P<0?05) . The tumor size in Lactobacillus rhamnosus group was reduced without significance. Tumor weight in Lactobacillus brevis group after two weeks was reduced by 36?47% compared to PBS group, which in Lactobacillus rhamnosus was down?regulated by 21?68%. In conclusion, mouse co?lon caner model was constructed and Lactobacillus brevis displayed better antitumor effect than Lac?tobacillus rhamnosus.