CAJ | 학술논문

目的：探究微 RNA ( miRNA )在乙型肝炎病毒感染不同时期表达谱的变化及其意义。方法选取2012年3月至2014年1月于临高县人民医院就诊的乙型肝炎病毒( HBV )感染者200例,其中急性乙型肝炎、慢性乙型肝炎、肝硬化、肝癌患者和健康对照组各50例,提取外周血中单个核细胞后,分离信使RNA,采用RNA-DNA嵌合探针液态芯片技术检测miR-20a、miR-21、miR-31、miR-145、miR-222、miR-191、miR-371、miR-223、miR-126的表达水平。结果不同时期HBV感染者外周血单个核细胞中miR-21、miR-31、miR-145、miR-222、miR-191、miR-371的表达水平比较,差异无统计学意义；miR-20a的表达水平随HBV 感染的发展而升高, miR-126随 HBV 感染的发展而降低, miR-223在急性乙型肝炎中的表达水平最高(15．44±8．52),在肝癌组中的表达水平最低(6．87±2．11),差异均有统计学意义(P<0．05)。结论 HBV感染发展不同时期的miRNA表达谱是不同的,通过检测相关miRNA的表达,可以为HBV感染的慢性化发展提供一个新的研究视角。
목적：탐구미 RNA ( miRNA )재을형간염병독감염불동시기표체보적변화급기의의。방법선취2012년3월지2014년1월우림고현인민의원취진적을형간염병독( HBV )감염자200례,기중급성을형간염、만성을형간염、간경화、간암환자화건강대조조각50례,제취외주혈중단개핵세포후,분리신사RNA,채용RNA-DNA감합탐침액태심편기술검측miR-20a、miR-21、miR-31、miR-145、miR-222、miR-191、miR-371、miR-223、miR-126적표체수평。결과불동시기HBV감염자외주혈단개핵세포중miR-21、miR-31、miR-145、miR-222、miR-191、miR-371적표체수평비교,차이무통계학의의；miR-20a적표체수평수HBV 감염적발전이승고, miR-126수 HBV 감염적발전이강저, miR-223재급성을형간염중적표체수평최고(15．44±8．52),재간암조중적표체수평최저(6．87±2．11),차이균유통계학의의(P<0．05)。결론 HBV감염발전불동시기적miRNA표체보시불동적,통과검측상관miRNA적표체,가이위HBV감염적만성화발전제공일개신적연구시각。
Objective To evaluate the expression profile and significance of miRNA different periods of HBV infection. Methods Two hundred patients infected by HBV from Lingao County People′s Hospital dur-ing Mar. 2012 and Jan. 2014 were selected in this study. Among them,there were 50 patients with acute hep-atitis B,50 with chronic hepatitis B,50 with hepatitis B liver cirrhosis,50 with liver cancer and 50 healthy control. The expression level of miR-20a,miR-21,miR-31,miR-145,miR-222,miR-191,miR-371,miR-223 and miR-126 were detected by xMap liquid chip technology after extracting mononuclear cells from peripheral blood. Results There were no statistically differences of miR-21miR-31, miR-145, miR-222, miR-191, miR-371 in different groups(P>0. 05). As the progression of the disease,the expression level of miR-20a increased significantly,while miR-126 decreased greatly. The expression level of miR-223 was the highest in acute hepatitis B group (15.44 ±8.52),while it was the lowest in the liver cancer group (6.87 ±2.11), the difference was statistically significant(P<0. 05). Conclusion The expression profile of miRNA in dif-ferent periods of HBV infection is different,which provides a new clue to the research of the mechanisms of the chronicity of HBV infection.