中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
39期
3180-3183
,共4页
脓毒症%肝功能衰竭,急性%白细胞介素12%罗格列酮
膿毒癥%肝功能衰竭,急性%白細胞介素12%囉格列酮
농독증%간공능쇠갈,급성%백세포개소12%라격렬동
Sepsis%Liver failure,acute%Interleukin-12%Rosiglitazone
目的 观察罗格列酮(ROSI)对脓毒症大鼠急性肝损伤的保护作用.方法 清洁级SD雄性大鼠共96只,数字表法随机分为脓毒症+ ROSI组、脓毒症+二甲基亚砜(DMSO)组、假手术+ROSI组、假手术组+DMSO组.模型建立完成后0、6、12、24 h每组分别处死6只大鼠.观察和比较各组大鼠之间的活动度及肝脏组织病理学变化,分离血清检测门冬氨酸氨基转移酶(GOT)、丙氨酸氨基转移酶(GPT)及白细胞介素12 (IL-12)的浓度.结果 12、24 h脓毒症+ROSI组与脓毒症+DMSO组及脓毒症+ROSI组与假手术+ROSI组大鼠之间GOT差异有统计学意义(t12 =3.97,P12=0.01;t24 =3.10,P24 ＜0.001),(t12=0.55,P12 ＜0.001;t24=4.32,P24＜0.001);6、12、24 h脓毒症+ROSI组与脓毒症+DMSO组及脓毒症+ROSI组与假手术+ROSI组大鼠之间IL-12差异有统计学意义(t6 =5.96,P6 ＜0.001;t12 =3.90,P12 =0.003;t24 =2.84,P24=0.018), (t6=0.48,P6 ＜0.001;t12=0.54,P12 =0.001;t24 =4.32,P24=0.009).6、12、24 h脓毒症+ROSI组与脓毒症+DMSO组及脓毒症+ ROSI组与假手术+ROSI组大鼠之间GPT差异有统计学意义(t6 =3.17,P6=0.021;t12=2.81,P12 =0.018;t24 =5.06,P24 ＜0.001), (t6 =2.67,P6 =0.001;t12 =3.36,P12 =0.002;t24 =2.83,P24＜0.001).血浆GOT、GPT及IL-12浓度在6h开始升高,24 h时为高值,未见明确峰值趋势.结论 噻唑烷二酮类药物罗格列酮有抑制脓毒症大鼠炎症因子IL-12表达的作用,降低肝损害程度,减轻炎症反应,对脓毒症大鼠急性肝损伤有保护作用.
目的 觀察囉格列酮(ROSI)對膿毒癥大鼠急性肝損傷的保護作用.方法 清潔級SD雄性大鼠共96隻,數字錶法隨機分為膿毒癥+ ROSI組、膿毒癥+二甲基亞砜(DMSO)組、假手術+ROSI組、假手術組+DMSO組.模型建立完成後0、6、12、24 h每組分彆處死6隻大鼠.觀察和比較各組大鼠之間的活動度及肝髒組織病理學變化,分離血清檢測門鼕氨痠氨基轉移酶(GOT)、丙氨痠氨基轉移酶(GPT)及白細胞介素12 (IL-12)的濃度.結果 12、24 h膿毒癥+ROSI組與膿毒癥+DMSO組及膿毒癥+ROSI組與假手術+ROSI組大鼠之間GOT差異有統計學意義(t12 =3.97,P12=0.01;t24 =3.10,P24 ＜0.001),(t12=0.55,P12 ＜0.001;t24=4.32,P24＜0.001);6、12、24 h膿毒癥+ROSI組與膿毒癥+DMSO組及膿毒癥+ROSI組與假手術+ROSI組大鼠之間IL-12差異有統計學意義(t6 =5.96,P6 ＜0.001;t12 =3.90,P12 =0.003;t24 =2.84,P24=0.018), (t6=0.48,P6 ＜0.001;t12=0.54,P12 =0.001;t24 =4.32,P24=0.009).6、12、24 h膿毒癥+ROSI組與膿毒癥+DMSO組及膿毒癥+ ROSI組與假手術+ROSI組大鼠之間GPT差異有統計學意義(t6 =3.17,P6=0.021;t12=2.81,P12 =0.018;t24 =5.06,P24 ＜0.001), (t6 =2.67,P6 =0.001;t12 =3.36,P12 =0.002;t24 =2.83,P24＜0.001).血漿GOT、GPT及IL-12濃度在6h開始升高,24 h時為高值,未見明確峰值趨勢.結論 噻唑烷二酮類藥物囉格列酮有抑製膿毒癥大鼠炎癥因子IL-12錶達的作用,降低肝損害程度,減輕炎癥反應,對膿毒癥大鼠急性肝損傷有保護作用.
목적 관찰라격렬동(ROSI)대농독증대서급성간손상적보호작용.방법 청길급SD웅성대서공96지,수자표법수궤분위농독증+ ROSI조、농독증+이갑기아풍(DMSO)조、가수술+ROSI조、가수술조+DMSO조.모형건립완성후0、6、12、24 h매조분별처사6지대서.관찰화비교각조대서지간적활동도급간장조직병이학변화,분리혈청검측문동안산안기전이매(GOT)、병안산안기전이매(GPT)급백세포개소12 (IL-12)적농도.결과 12、24 h농독증+ROSI조여농독증+DMSO조급농독증+ROSI조여가수술+ROSI조대서지간GOT차이유통계학의의(t12 =3.97,P12=0.01;t24 =3.10,P24 ＜0.001),(t12=0.55,P12 ＜0.001;t24=4.32,P24＜0.001);6、12、24 h농독증+ROSI조여농독증+DMSO조급농독증+ROSI조여가수술+ROSI조대서지간IL-12차이유통계학의의(t6 =5.96,P6 ＜0.001;t12 =3.90,P12 =0.003;t24 =2.84,P24=0.018), (t6=0.48,P6 ＜0.001;t12=0.54,P12 =0.001;t24 =4.32,P24=0.009).6、12、24 h농독증+ROSI조여농독증+DMSO조급농독증+ ROSI조여가수술+ROSI조대서지간GPT차이유통계학의의(t6 =3.17,P6=0.021;t12=2.81,P12 =0.018;t24 =5.06,P24 ＜0.001), (t6 =2.67,P6 =0.001;t12 =3.36,P12 =0.002;t24 =2.83,P24＜0.001).혈장GOT、GPT급IL-12농도재6h개시승고,24 h시위고치,미견명학봉치추세.결론 새서완이동류약물라격렬동유억제농독증대서염증인자IL-12표체적작용,강저간손해정도,감경염증반응,대농독증대서급성간손상유보호작용.
Objective To observe the effects of rosiglitazone (ROSI) on endotoxin-induced acute liver injury in rats.Methods All the animals were randomly assigned to one of four following groups (n =24 per group).Rats in the Sepsis-Dimethyl sulfoxide (DMSO) group(n =24), rats were treated with 10％ DMSO(1 ml/kg) 30 mins before they were challenged with cecal ligation and puncture(CLP) (n =24).In the sepsis-rosiglitazone (ROSI) group was identical to the sepsis-DMSO group but received ROSI (10 mg/kg intravenously) (n =24).In the sham-DMSO group, rats were treated with 10％ DMSO(1 ml/kg) 30 mins before the administration of saline (n =24).The sham-ROSI group was identical to the sham-ROSI group except that ROSI (10 mg/kg intravenously) was administered instead of DMSO (n =24).Sepsis was induced in rats by dissecting the cecum and placing the discontinued organ back into the abdomen CLP.And all the Rats were decapitated at 0,6, 12,24 h after the model completed.Serum glutamic oxaloacetic transaminase(GOT), glutamic pyruvic transaminase (GPT), interleukin 12 (IL-12) were determined to assess activity and liver pathologic change of rats in different groups.Data were analyzed with SPSS 19.0 software.Student's t-test was used to compare densitometry data.Results SPSS 19.0 statistical software analysis by the outcome of the application ELASA method for determining zero hours between every group, there was non-difference.The sepsis-ROSI group and the sepsis-DMSO group 12 hours and 24 hours the presence of plasma GOT concentration changes statistically significant difference (t12 =3.97, P12 =0.01;t24 =3.10,Pu ＜ 0.001), (t12 =0.55, P12 ＜ 0.001;t24 =4.32, P24 ＜ 0.001).The Sepsis-ROSI group and the sepsis-DMSO group 6 hours, 12 hours and 24 hours the presence of plasma GPT concentration changes statistically significant difference(t6 =3.17 ,P6 =0.021;t12 =2.81, P12 =0.018;t24 =5.06,P24 ＜0.001), (t6 =2.67 ,P6 =0.001;t12 =3.36,P12 =0.002;t24 =2.83, P24 ＜ 0.001).The sepsis-ROSI group and the sepsis-DMSO group 6 hours, 12 hours and 24 hours the presence of plasma IL-12 concentration changes statistically significant difference(t6 =3.17,P6 =0.021;t12 =2.81 ,P12 =0.018;t24 =5.06,P24 ＜0.001),(t6 =2.67, P6 =0.001;t12 =3.36,P12 =0.002;t24 =2.83,P24 ＜0.001).Sepsis GOT,GPT, IL-12 began to increase in 6 hours, 24 hours of high-value, no clear peak trend, the sepsis-ROSI group compared with the sham-ROSI group had lower concentrations of GOT, GPT and IL-12 to some extent.Description rosiglitazone inhibit inflammatory cytokines in rats with sepsis role of IL-12, there is a certain role to reduce the inflammatory response in sepsis.Conclusions Treatment with Thiazolidinedione, markedly inhibited the expression of IL-12, attenuated pulmonary inflammation and liver injury caused by sepsis in rat.
