CAJ | 학술논문

拟检测林蛙皮多肽对大鼠皮肤激光损伤的修复作用及初步探索其作用途径。用激光照射大鼠背部皮肤，采用不同浓度林蛙皮多肽处理创面，观察其形态学变化，统计创面愈合率；检测Flg（参与表皮屏障功能建立的重要蛋白质之一）和Krt?14 mRNA（参与形成上皮细胞的细胞骨架）的差异。结果表明：第3、6天，低浓度（10 mg／mL）林蛙皮多肽作用组大鼠激光损伤皮肤愈合率显著高于阴性对照组（P＜0?05），第6天，低浓度林蛙多肽作用组大鼠创伤皮肤中Flg的表达量是阴性对照组的1?908倍（P＜0?0001），Krt?14的表达量是阴性对照组的1?453倍（ P＜0?0001）。这表明10 mg／mL林蛙皮多肽对大鼠激光损伤皮肤有修复作用，可能是通过促进损伤细胞中Flg和Krt?14 mRNA表达量的增加而发挥修复作用的。
의검측림와피다태대대서피부격광손상적수복작용급초보탐색기작용도경。용격광조사대서배부피부，채용불동농도림와피다태처리창면，관찰기형태학변화，통계창면유합솔；검측Flg（삼여표피병장공능건립적중요단백질지일）화Krt?14 mRNA（삼여형성상피세포적세포골가）적차이。결과표명：제3、6천，저농도（10 mg／mL）림와피다태작용조대서격광손상피부유합솔현저고우음성대조조（P＜0?05），제6천，저농도림와다태작용조대서창상피부중Flg적표체량시음성대조조적1?908배（P＜0?0001），Krt?14적표체량시음성대조조적1?453배（ P＜0?0001）。저표명10 mg／mL림와피다태대대서격광손상피부유수복작용，가능시통과촉진손상세포중Flg화Krt?14 mRNA표체량적증가이발휘수복작용적。
This study was to detect repair effect of Rana skin polypeptides on rat skin laser injury and to explore preliminarily its effect path. Dorsal skin of rats was irradiated with laser, different concentrations of polypeptide were used to treat Rana skin wounds, morphological changes were ob?served, and wound healing rate was statistically surveyed;Flg ( one of the major proteins involved in the establishment of epidermal barrier function) and Krt?14 mRNA ( cell cytoskeleton involved in the formation of epithelial cells) levels were detected;As a result, on the first 3 to 6 days, healing rate of rat skin with laser?induced damage in low?concentration ( 10 mg/mL ) Rana skin polypeptide effect group was significantly higher than that of the negative control group ( P<0?05) , on the first 6 days, the role of low?concentration Rana polypeptide expression level of rat skin wound Flg is 1?908 times that of the negative control group ( P<0?000 1) , the expression level of Krt?14 is 1?453 times that of the negative control group ( P<0?000 1 ) . These results suggest that 10 mg/mL Rana skin polypeptides have repair effect on laser?induced damage of rat skin, and the repair effect may be re?alized by promoting Flg and Krt?14 mRNA expression levels of the injured cells.