CAJ | 학술논문

目的：观察银杏叶提取物对非酒精性脂肪肝炎大鼠Toll样受体4（TLR4）、肿瘤坏死因子α（TNF‐α）等表达的影响。方法雄性SD大鼠分为3组。对照组正常饮食，模型组、治疗组给予高脂饲料喂养16周诱发脂肪性肝炎后分别以生理盐水、银杏叶提取物液10 mL／kg灌胃，每日1次，共8周。处死大鼠，分别进行如下检测：（1）观察肝脏病理变化；（2）检测 TLR4表达水平；（3）检测血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）水平及血清TNF‐α水平。结果（1）治疗组和模型组均有不同程度的肝细胞脂肪变性，且治疗组轻于模型组。（2）模型组、治疗组TLR4 mRNA及蛋白、TNF‐α表达高于对照组，模型组又高于治疗组，差异有统计学意义。对照组血清ALT、AST明显低于模型组及治疗组，治疗组又低于模型组，差异有统计学意义（P＜0．05）。结论银杏叶提取物对非酒精性脂肪肝炎大鼠有较好的肝脏保护作用，其可能机制与降低 TLR4的表达、抑制炎性反应有关。
목적：관찰은행협제취물대비주정성지방간염대서Toll양수체4（TLR4）、종류배사인자α（TNF‐α）등표체적영향。방법웅성SD대서분위3조。대조조정상음식，모형조、치료조급여고지사료위양16주유발지방성간염후분별이생리염수、은행협제취물액10 mL／kg관위，매일1차，공8주。처사대서，분별진행여하검측：（1）관찰간장병리변화；（2）검측 TLR4표체수평；（3）검측혈청병안산안기전이매（ALT）、천문동안산안기전이매（AST）수평급혈청TNF‐α수평。결과（1）치료조화모형조균유불동정도적간세포지방변성，차치료조경우모형조。（2）모형조、치료조TLR4 mRNA급단백、TNF‐α표체고우대조조，모형조우고우치료조，차이유통계학의의。대조조혈청ALT、AST명현저우모형조급치료조，치료조우저우모형조，차이유통계학의의（P＜0．05）。결론은행협제취물대비주정성지방간염대서유교호적간장보호작용，기가능궤제여강저 TLR4적표체、억제염성반응유관。
Objective To observe the effect of ginkgo biloba extract on the expression of TLR4 and TNF‐αin rat nonalcohol‐ic steatohepatitis .Methods SD rats were randomly divided into three groups :control group (normal diet) ,rats in the model group and the treatment group was given high fat diet to induce steatohepatitis for 16 weeks ,respectively .Rats in model group were trea‐ted with normal saline ,rats in treatment group was fed ginkgo biloba extract (10 mL/kg per day ,for eight weeks) .Then rats were sacrificed ,the following tests were performed:(1)The pathological changes in liver;(2)the expression level of TLR4 ;(3)serum ALT ,AST levels and serum TNF‐α levels .Results (1)Both treatment group and model group have different levels of hepatic steatosis ,and it was lighter in the treatment group .(2)TLR4 mRNA and its protein and TNF‐α expression in model group and treatment group were higher than that of the control group ,and the model group was higher than the treatment group ,the differ‐ence was statistically significant .Serum ALT and AST was significantly lower in control group than the model group and the treat‐ment group ,the treatment group was lower than model group ,the difference was statistically significant (P<0 .05) .Conclusion Ginkgo biloba extract has good protection effect on nonalcoholic steatohepatitis in rats ,and the possible mechanism may be the re‐duction of the expression of TLR4 and the inhibition of inflammatory reaction .