CAJ | 학술논문

目的：研究健康SD鼠在妊娠和哺乳期肝脏胆酸代谢基因的变化。方法取孕10、14、19 d及产后1、7、14、21 d的大鼠肝脏，检测胆酸水平和肝脏法尼醇X受体（FXR）、小异二聚体伴侣受体（SHP）、胆固醇7α‐羟化酶（Cyp7α1）的表达。用West‐ern blot法测Cyp7α1的蛋白表达情况。结果孕晚期健康大鼠，肝脏中胆酸及Cyp7α1表达不升高；哺乳期的大鼠，肝脏胆酸增加，FXR表达升高；在孕晚期及哺乳期靶基因SHP表达均高于对照组，且其表达高峰期是第孕19天，其值可达对照组的6倍之多。结论健康大鼠在产后肝脏胆酸合成增加，而孕期减少，可能与Cyp7α1、FXR及SHP表达有关。
목적：연구건강SD서재임신화포유기간장담산대사기인적변화。방법취잉10、14、19 d급산후1、7、14、21 d적대서간장，검측담산수평화간장법니순X수체（FXR）、소이이취체반려수체（SHP）、담고순7α‐간화매（Cyp7α1）적표체。용West‐ern blot법측Cyp7α1적단백표체정황。결과잉만기건강대서，간장중담산급Cyp7α1표체불승고；포유기적대서，간장담산증가，FXR표체승고；재잉만기급포유기파기인SHP표체균고우대조조，차기표체고봉기시제잉19천，기치가체대조조적6배지다。결론건강대서재산후간장담산합성증가，이잉기감소，가능여Cyp7α1、FXR급SHP표체유관。
Objective To investigate the accumulation of liver bile acids and bile acid regulated nuclear receptor farnesoid X (FXR) and its target gene cholesterol 7α hydroxylase (Cyp7α1) and small different dimer partner (SHP) in normal rats during pregnancy and lactation .Methods SD rats were maintained in the SPF‐grade facilities for time‐controlled mate .Livers were collect‐ed at Gestation Day (GD) 10 ,14 ,and 19 and Postnatal Day (PND) 1 ,7 ,14 and 21 d .Hepatic bile acids were quantified with a total bile acid assay kit ,and mRNA expression of FXR ,Cyp7α1 and SHP was determined by RT‐PCR .Western blot was used for Cyp7α1 protein quantification .Results In livers of normal rats ,bile acids was not elevated during pregnancy but was increased during lacta‐tion .The bile acid synthesis rate limiting enzyme Cyp7α1 increased during lactation but were normal during pregnancy .SHP was 6 fold higher at the late pregnancy ,and slightly higher during lactation .FXR was increased at postpartum .Conclusion Under normal circumstances ,hepatic bile acid synthesis during pregnancy was controlled by SHP feedback ,while during lactation ,hepatic bile acids increased ,along with increased Cyp7α1 and FXR .