CAJ | 학술논문

目的：探讨T淋巴细胞亚群CD3＋、CD4＋、CD8＋、CD4＋／CD8＋比值和CD55＋、CD59＋水平在各种贫血患者外周血中的表达及意义。方法利用流式细胞术，测定100例贫血患者，其中巨幼细胞贫血（M A ）25例，缺铁性贫血（ID A ）40例，再生障碍性贫血（A A ）35例，30名健康对照组外周血T淋巴细胞亚群（CD3＋、CD4＋、CD8＋和CD4＋／CD8＋比值）和CD55＋、CD59＋水平的变化，比较与健康对照组间的差异。结果与健康对照组相比，3类贫血患者的CD3＋、CD4＋和CD4＋／CD8＋均低于健康对照组[（69．2±3．62）％、（38．7±3．56）％、（1．69±0．15）％]，其中A A患者的CD4＋／CD8＋比值为0．94±0．59，与健康对照组相比明显下降（ P＜0．05）；CD8＋均高于健康对照组（22．9±2．35）％，其中A A患者的CD8＋为（34．5±2．44）％，明显高于健康对照组（P＜0．05）；MA患者CD55＋和CD59＋表达正常，大约40％的IDA和34％的AA患者CD55＋和CD59＋表达异常。结论3类贫血患者免疫功能均有不同程度异常，IDA患者可能是由缺铁引起。AA患者可能是由 T细胞功能亢进和CD55＋、CD59＋表达异常引起的。
목적：탐토T림파세포아군CD3＋、CD4＋、CD8＋、CD4＋／CD8＋비치화CD55＋、CD59＋수평재각충빈혈환자외주혈중적표체급의의。방법이용류식세포술，측정100례빈혈환자，기중거유세포빈혈（M A ）25례，결철성빈혈（ID A ）40례，재생장애성빈혈（A A ）35례，30명건강대조조외주혈T림파세포아군（CD3＋、CD4＋、CD8＋화CD4＋／CD8＋비치）화CD55＋、CD59＋수평적변화，비교여건강대조조간적차이。결과여건강대조조상비，3류빈혈환자적CD3＋、CD4＋화CD4＋／CD8＋균저우건강대조조[（69．2±3．62）％、（38．7±3．56）％、（1．69±0．15）％]，기중A A환자적CD4＋／CD8＋비치위0．94±0．59，여건강대조조상비명현하강（ P＜0．05）；CD8＋균고우건강대조조（22．9±2．35）％，기중A A환자적CD8＋위（34．5±2．44）％，명현고우건강대조조（P＜0．05）；MA환자CD55＋화CD59＋표체정상，대약40％적IDA화34％적AA환자CD55＋화CD59＋표체이상。결론3류빈혈환자면역공능균유불동정도이상，IDA환자가능시유결철인기。AA환자가능시유 T세포공능항진화CD55＋、CD59＋표체이상인기적。
Objective To investigate the clinical significance and expression of peripheral blood T lymphocyte subset CD3+ , CD4+ ,CD8+ and CD4+ /CD8+ ratio and CD55+ ,CD59+ in patients with anemia .Methods Peripheral blood T lymphocyte subset CD3+ ,CD4+ ,CD8+ ,CD4+ /CD8+ and CD55+ ,CD59+ ratio were determined by flow cytometry in a total of 100 patients with ane‐mia(including 25 patients with MA ,40 patients with IDA ,35 patients with AA and 30 healthy controls were enrolled) .The differ‐ences between the anemia group and the healthy control group were analyzed statistics .Results The peripheral blood T lymphocyte subset CD3+ ,CD4+ and CD4+ /CD8+ ratio in three groups of anemia patients [(69 .2 ± 3 .62)% ,(38 .7 ± 3 .56)% ,(1 .69 ± 0 .15)% ] were lower than the healthy control group .The ratio of CD4+ /CD8+ in AA patients(0 .94 ± 0 .59) was the lowest(P<0 .05) .The level of CD8+ in three groups of anemia patients were higher than the healthy control group(22 .9 ± 2 .35)% ,and CD8+in AA patients(34 .5 ± 2 .44)% was the highest(P<0 .05) .The expression of CD55+ and CD59+ was normal in MA patients ,but was abnormal in approximately 40% IDA and 34% AA patients .Conclusion The immune function of three kinds of anemia patient were abnormal ,abnormal immune function of IDA and AA patients may be caused directly by the iron and T‐lymphocyte ,CD55+ , CD59+ expression abnormal dependently .