重庆医学
重慶醫學
중경의학
Chongqing Medicine
2015年
30期
4229-4231
,共3页
妊娠期高血压疾病%大电导钙激活钾通道%α亚基%β1 亚基
妊娠期高血壓疾病%大電導鈣激活鉀通道%α亞基%β1 亞基
임신기고혈압질병%대전도개격활갑통도%α아기%β1 아기
hypertensive disorder complicating pregnancy%large-conductance calcium activated potassium channels%α-sub-unit%β1-subunit
目的：观察妊娠期高血压疾病（HDCP）孕妇胎盘小动脉中大电导钙激活钾通道（BKCa）的蛋白表达，探讨BKCa在该疾病发生机制中的作用。方法取 HDCP孕妇（HDCP组，＝15）与健康孕妇（NT组，n＝15）胎盘小动脉平滑肌，用蛋白免疫印迹法（Western blot）检测BKCa通道α亚基及β1亚基的蛋白水平。结果胎盘小动脉平滑肌检测显示，BKCa通道α亚基在NT组的蛋白相对表达量为1．0282±0．1806，HDCP组为1．0012±0．1698，差异无统计学意义（P＞0．05）；β1亚基在NT组的蛋白相对表达量为1．6168±0．0126，HDCP组为0．4181±0．0808，差异有统计学意义（P＜0．05）。结论 HDCP胎盘小动脉平滑肌BKCa通道α亚基蛋白表达无明显变化，β1亚基蛋白表达均明显下调。由此推测大电导钙激活钾通道可能参与了 HDCP的发生、发展，β1亚基的异常表达可能是导致HDCP血管舒缩功能障碍的一个重要基础。
目的：觀察妊娠期高血壓疾病（HDCP）孕婦胎盤小動脈中大電導鈣激活鉀通道（BKCa）的蛋白錶達，探討BKCa在該疾病髮生機製中的作用。方法取 HDCP孕婦（HDCP組，＝15）與健康孕婦（NT組，n＝15）胎盤小動脈平滑肌，用蛋白免疫印跡法（Western blot）檢測BKCa通道α亞基及β1亞基的蛋白水平。結果胎盤小動脈平滑肌檢測顯示，BKCa通道α亞基在NT組的蛋白相對錶達量為1．0282±0．1806，HDCP組為1．0012±0．1698，差異無統計學意義（P＞0．05）；β1亞基在NT組的蛋白相對錶達量為1．6168±0．0126，HDCP組為0．4181±0．0808，差異有統計學意義（P＜0．05）。結論 HDCP胎盤小動脈平滑肌BKCa通道α亞基蛋白錶達無明顯變化，β1亞基蛋白錶達均明顯下調。由此推測大電導鈣激活鉀通道可能參與瞭 HDCP的髮生、髮展，β1亞基的異常錶達可能是導緻HDCP血管舒縮功能障礙的一箇重要基礎。
목적：관찰임신기고혈압질병（HDCP）잉부태반소동맥중대전도개격활갑통도（BKCa）적단백표체，탐토BKCa재해질병발생궤제중적작용。방법취 HDCP잉부（HDCP조，＝15）여건강잉부（NT조，n＝15）태반소동맥평활기，용단백면역인적법（Western blot）검측BKCa통도α아기급β1아기적단백수평。결과태반소동맥평활기검측현시，BKCa통도α아기재NT조적단백상대표체량위1．0282±0．1806，HDCP조위1．0012±0．1698，차이무통계학의의（P＞0．05）；β1아기재NT조적단백상대표체량위1．6168±0．0126，HDCP조위0．4181±0．0808，차이유통계학의의（P＜0．05）。결론 HDCP태반소동맥평활기BKCa통도α아기단백표체무명현변화，β1아기단백표체균명현하조。유차추측대전도개격활갑통도가능삼여료 HDCP적발생、발전，β1아기적이상표체가능시도치HDCP혈관서축공능장애적일개중요기출。
Objective To observe the change of protein levels of large conductance calcium activated potassium channel (BK‐Ca) in placental arteriole smooth muscle cells from hypertensive disorder complicating pregnancy and discuss its role .Methods Western blot analysis was used to examine protein expression of α subunit andβ1 subunit of BKCa channels in placental arteriole smooth muscle cells from hypertensive disorder complicating pregnancy .Results The relative protein expression level of α‐subunit in the HDCP group was 1 .001 2 ± 0 .169 8(n=15) ,and the NT group was 1 .028 2 ± 0 .180 6 (n=15) .There was no significant differences between the two groups (P> 0 .05);the relative protein expression level of β1 subunit in the HDCP group was 0 .418 1 ± 0 .080 8 (n=15) ,and the NT group was 1 .616 8 ± 0 .012 6 (n=15) ,theβ1‐subunit protein expression levels of HDCP group were significantly lower (P<0 .05) .Conclusion The protein expression ofβ1‐subunit ,but notα‐subunit ,was reduced in pla‐cental arteriole smooth muscle cells from hypertensive disorder complicating pregnancy .Therefore ,BKCa channel activity may have been involved in hypertensive disorder complicating pregnancy ;and the abnormal expression ofβ1 subunit maybe an important basis in hypertensive disorder complicating pregnancy .
