CAJ | 학술논문

目的：通过对哮喘小鼠进行研究，建立其气道重塑模型，同时运用1，25‐二羟基维生素D3[1，25‐（O H ）2 D3]进行干预，哮喘小鼠脾脏内及外周血髓系抑制细胞（MDSCs）水平及气道壁厚度的改变进行检测，分析1，25‐（OH）2D3对MDSCs及气道炎症的影响，从而了解其在哮喘发病机制的作用。方法选取30只BALB／c系小鼠，雌性、健康、8周龄，将其随机分为3组，分别为哮喘组，对照组及1，25‐（OH）2D3干预组，通过卵清蛋白致敏、激发建立哮喘模型。取肺组织进行 HE染色，比较小鼠气道壁厚度；收集各组小鼠外周血及脾脏细胞，采用流式细胞技术方法测定其CD11b＋ Gr1＋ MDSCs细胞的比例。结果哮喘组小鼠气道发生典型气道重塑的改变，小鼠体内MDSCs均较对照组、1，25‐（OH）2D3干预组下降；1，25‐（OH）2D3干预组较哮喘组气道重塑有所改善，MDSCs较对照组、哮喘组升高，差异有统计学意义（P＜0．05）。结论1，25‐（OH）2D3可能对于哮喘的发作起到一定的治疗的作用，其可能通过对于MDSCs的水平的上调来发挥作用，使得气道的炎症得以减轻，同时能够对气道重塑进行改善。
목적：통과대효천소서진행연구，건립기기도중소모형，동시운용1，25‐이간기유생소D3[1，25‐（O H ）2 D3]진행간예，효천소서비장내급외주혈수계억제세포（MDSCs）수평급기도벽후도적개변진행검측，분석1，25‐（OH）2D3대MDSCs급기도염증적영향，종이료해기재효천발병궤제적작용。방법선취30지BALB／c계소서，자성、건강、8주령，장기수궤분위3조，분별위효천조，대조조급1，25‐（OH）2D3간예조，통과란청단백치민、격발건립효천모형。취폐조직진행 HE염색，비교소서기도벽후도；수집각조소서외주혈급비장세포，채용류식세포기술방법측정기CD11b＋ Gr1＋ MDSCs세포적비례。결과효천조소서기도발생전형기도중소적개변，소서체내MDSCs균교대조조、1，25‐（OH）2D3간예조하강；1，25‐（OH）2D3간예조교효천조기도중소유소개선，MDSCs교대조조、효천조승고，차이유통계학의의（P＜0．05）。결론1，25‐（OH）2D3가능대우효천적발작기도일정적치료적작용，기가능통과대우MDSCs적수평적상조래발휘작용，사득기도적염증득이감경，동시능구대기도중소진행개선。
Objective To study the establishment of airway remodeling model ,while the use of 1 ,25‐(OH)2D3 to intervene in the asthmatic mice spleen and peripheral blood myeloid suppressor cells (myeliod‐derived suppressor cells ,MDSCs) level and changes in airway wall thickness were detected .It aims to analyze the 1 ,25‐(OH)2D3 MDSCs and influence on airway inflamma‐tion ,to understand its role in the pathogenesis of asthma .Methods Totally 30 female mice(BALB/c strain) were randomly divided into control group ,asthmatic group and asthmatic1 ,25‐(OH)2D3 group and built asthmatic mouse model .The pulmonary histologi‐cal difference indicative of respiratory tract remodeling among groups were observed via HE staining .The proportion of MDSCs within the peripheral blood and spleen was tested by flow cytometry .Results Typical respiratory tract remodeling were found in asthmatic group with which MDSCs in the peripheral blood and spleen reduced ,asthmatic1 ,25‐(OH)2D3 group comparatively in‐creased ,there had significant difference(P< 0 .05) .Conclusion 1 ,25‐(OH)2D3 may play a role in asthma attacks for a certain treatment ,it may by regulation for MDSCs levels come into play ,so that airway inflammation can be reduced ,at the same time be a‐ble to airway remodeling improvement .