CAJ | 학술논문

目的：观察人华通胶间充质干细胞（WJ‐M HCs）对心肌梗死后心力衰竭大鼠肿瘤坏死因子α（TNF‐α）及N末端B型脑利钠肽前体（NT‐proBNP）的影响。方法采用80只雄性SD大鼠通过异丙肾上腺素多点皮下注射，剂量为200 mg／kg ，隔24 h注射1次共2次。待大鼠存活1周建立模型后各取12只随机均衡分入WJ‐M HCs移植组、普通对照组、空白对照组各12只健康大鼠，3组再分为移植前和移植后4周两个亚组。WJ‐M HCs移植组于心肌梗死后1周移植以DAPI标记的WJ‐M HCs ，空白对照组及普通对照组不做处理正常饲养。分别于移植前和移植后4周检测大鼠心脏组织中 TNF‐α水平，移植后4周WJ‐M HCs细胞在大鼠心脏中的情况，大鼠TNF‐α和NT‐proBNP的水平，左室射血分数（LVEF）。结果移植后WJ‐M HCs移植组较移植前LVEF明显提高（P＜0．05），较移植前及普通对照组血清 TNF‐α及NT‐proBNP明显降低（P＜0．05）；移植后WJ‐MHCs移植组较普通对照组心脏组织中TNF‐α表达明显减少；WJ‐M HCs移植组病死率（16．67％）较普通对照组病死率（33．33％）明显降低；免疫荧光显示移植后4周仍可检测到移植的WJ‐M HCs。结论移植人WJ‐M HCs可明显降低心梗后心衰大鼠心脏组织及循环中的TNF‐α，降低循环中的NT‐proBNP ，提高心功能。
목적：관찰인화통효간충질간세포（WJ‐M HCs）대심기경사후심력쇠갈대서종류배사인자α（TNF‐α）급N말단B형뇌리납태전체（NT‐proBNP）적영향。방법채용80지웅성SD대서통과이병신상선소다점피하주사，제량위200 mg／kg ，격24 h주사1차공2차。대대서존활1주건립모형후각취12지수궤균형분입WJ‐M HCs이식조、보통대조조、공백대조조각12지건강대서，3조재분위이식전화이식후4주량개아조。WJ‐M HCs이식조우심기경사후1주이식이DAPI표기적WJ‐M HCs ，공백대조조급보통대조조불주처리정상사양。분별우이식전화이식후4주검측대서심장조직중 TNF‐α수평，이식후4주WJ‐M HCs세포재대서심장중적정황，대서TNF‐α화NT‐proBNP적수평，좌실사혈분수（LVEF）。결과이식후WJ‐M HCs이식조교이식전LVEF명현제고（P＜0．05），교이식전급보통대조조혈청 TNF‐α급NT‐proBNP명현강저（P＜0．05）；이식후WJ‐MHCs이식조교보통대조조심장조직중TNF‐α표체명현감소；WJ‐M HCs이식조병사솔（16．67％）교보통대조조병사솔（33．33％）명현강저；면역형광현시이식후4주잉가검측도이식적WJ‐M HCs。결론이식인WJ‐M HCs가명현강저심경후심쇠대서심장조직급순배중적TNF‐α，강저순배중적NT‐proBNP ，제고심공능。
Objective To observe the influence of human umbilical cord wharton′s jelly‐mesenchymal stem cells(WJ‐MHCs) on the tumor necrosis factorα (TNF‐α) and N‐terminal pro‐brain natriuretic peptide(NT‐proBNP) in rats with heart failure of a‐cute myocardial infarction .Methods Totally 80 male rat models of heart failure of acute myocardial infarction were made by isopre‐naline(ISO) 200 mg/kg injected subcutaneously twice at an interval of 24 hours .After one week ,24 survival rats were randomly di‐vided into WJ‐M HCs transplantation group and normal control group .Sham group was made of 12 health rats ,and then each of the three groups was subdivided into pre‐transplantation group and post‐transplantation group 4 weeks later .WJ‐MHCs transplantation group was transplanted with WJ‐MHCs with DAPI labeled after ISO injected one week .Sham group and normal group were un‐treated and normally bred .The left ventricular ejection fraction(LVEF) measured by before transplantation and post‐transplantation 4 weeks later .The injected cells and the expression of TNF‐αwas measured .Results Compared to pre‐transplantation group ,WJ‐M HCs transplantation group increased the LVEF(P<0 .05);compared to pre‐transplantation and normal control ,WJ‐M HCs trans‐plantation group reduced the TNF‐αand NT‐proBNP in the serum(P<0 .05)and the expression of TNF‐α from the heart tissue (P<0 .05);compared to normal transplantation ,WJ‐M HCs transplantation group reduced the mortality from 33 .3% to 16 .7% ;immunofluorescence demonstrated that transplanted cells were still found alive in the heart after transplantation 4 weeks later .Con‐clusion Transplantation of WJ‐MHCS down‐regulates TNF‐α and NT‐proBNP in the serum in the serum and the expression of TNF‐αfrom the heart tissue and up‐regulates the LVEF in rats with heart failure of acute myocardial infarction .