CAJ | 학술논문

目的：通过观察老年慢性阻塞性肺疾病（COPD）急性加重期、稳定期患者和健康老年人外周血中CD4＋ CD25＋Foxp3＋调节性T细胞（Treg）比例变化及COPD患者血清中转化生长因子‐β1（TGF‐β1）水平的相关性，探讨Treg、TGF‐β1在老年COPD发病中的作用。方法选择2012年3月至2014年2月在该院老年科的COPD急性加重期患者26例（急性期组）、稳定期患者23例（稳定期组），选择同期20例健康体检者为对照组，用流式细胞仪检测外周血中T reg表达比例，用ELISA法检测血清TGF‐β1。结果3组受检者外周血Treg比例，各病例组与对照组比较差异有统计学意义（P＜0．01），急性期组与稳定期组比较差异有统计学意义（P＜0．05）；TGF‐β1水平，急性期组与对照组比较差异有统计学意义（P＜0．01）；各组Treg与TGF‐β1均无相关性（P＞0．05）。结论 Treg细胞在老年COPD急性加重过程中有重要作用，但 Treg与 TGF‐β1无明显相关性，老年患者体内存在免疫功能紊乱或异常。
목적：통과관찰노년만성조새성폐질병（COPD）급성가중기、은정기환자화건강노년인외주혈중CD4＋ CD25＋Foxp3＋조절성T세포（Treg）비례변화급COPD환자혈청중전화생장인자‐β1（TGF‐β1）수평적상관성，탐토Treg、TGF‐β1재노년COPD발병중적작용。방법선택2012년3월지2014년2월재해원노년과적COPD급성가중기환자26례（급성기조）、은정기환자23례（은정기조），선택동기20례건강체검자위대조조，용류식세포의검측외주혈중T reg표체비례，용ELISA법검측혈청TGF‐β1。결과3조수검자외주혈Treg비례，각병례조여대조조비교차이유통계학의의（P＜0．01），급성기조여은정기조비교차이유통계학의의（P＜0．05）；TGF‐β1수평，급성기조여대조조비교차이유통계학의의（P＜0．01）；각조Treg여TGF‐β1균무상관성（P＞0．05）。결론 Treg세포재노년COPD급성가중과정중유중요작용，단 Treg여 TGF‐β1무명현상관성，노년환자체내존재면역공능문란혹이상。
Objective To observe the expression level of CD4+ CD25+ Foxp3+ regulatory T cells in the peripheral blood of acute and stable senile COPD patients ,and analyze the correlation between Treg cells and TGF‐β1 of senile COPD ,then investigate the role of Treg cells and TGF‐β1 in the onset of senile COPD .Methods Totally 26 patients with acute stage and 23 patients with stable stage were investigated as acute group and stable group ,they came from the department of geriatric of our hospital form March ,2012 to February ,2014 .Meanwhile ,20 healthy people were selected as control group .The proportion of Treg cells in pe‐ripheral blood was measured by flow cytometry method and the level of TGF‐β1 in serum was measured by ELISA .Results The percentage of Treg on peripheral blood in acute and stable groups were significantly higher than control group(P< 0 .01) ,the difference also existed in acute and stable group(P<0 .05);the level of TGF‐β1 in serum of acute group was higher than control group(P<0 .01) ,there was no correlation between the proportion of Treg and TGF‐β1(P>0 .05) .Conclusion Treg cells may be involved in the process of the pathogenesis of senile COPD and acute exacerbation .There is no correlation between the proportion of Treg cells and TGF‐β1 ,and it indicates immune disorders may exist in senile COPD patients .