中华灾害救援医学
中華災害救援醫學
중화재해구원의학
Chinese Journal of Disaster Medicine
2015年
10期
568-571
,共4页
芦丁%柿叶提取物%药动学%高效液相色谱法
蘆丁%柿葉提取物%藥動學%高效液相色譜法
호정%시협제취물%약동학%고효액상색보법
rutin%extract of persimmon leaves%pharmacokinetics%HPLC
目的 建立高效液相色谱法(high performance liquid chromatography,HPLC)测定柿叶提取物中芦丁在大鼠体内的血药浓度,并探讨其在大鼠体内药物代谢动力学(以下简称药动学)特征.方法 SD大鼠给予柿叶提取物灌胃,在不同时间点眼后静脉丛取血,预处理后测定血浆中芦丁的浓度,经3P97软件处理数据.结果 柿叶提取物中芦丁在大鼠体内的药动学符合二房室模型,芦丁在体内较快地被吸收,给药后1 h即达到峰浓度,峰浓度Cmax为(1.658±0.184) mg/L,其他主要药动学参数吸收速率常数Ka=(2.436±0.883)/h、分布速率常数α=(1.002±0.372)/h、消除速率常数β=(0.083±0.067)/h、消除半衰期t1/2β=(9.418±7.256)h、药-时曲线下面积AUC0-12=(9.628±2.281)mg·h/L.结论 高效液相色谱法测定血浆中芦丁浓度准确、简便,适用于柿叶提取物中芦丁的药动学研究.
目的 建立高效液相色譜法(high performance liquid chromatography,HPLC)測定柿葉提取物中蘆丁在大鼠體內的血藥濃度,併探討其在大鼠體內藥物代謝動力學(以下簡稱藥動學)特徵.方法 SD大鼠給予柿葉提取物灌胃,在不同時間點眼後靜脈叢取血,預處理後測定血漿中蘆丁的濃度,經3P97軟件處理數據.結果 柿葉提取物中蘆丁在大鼠體內的藥動學符閤二房室模型,蘆丁在體內較快地被吸收,給藥後1 h即達到峰濃度,峰濃度Cmax為(1.658±0.184) mg/L,其他主要藥動學參數吸收速率常數Ka=(2.436±0.883)/h、分佈速率常數α=(1.002±0.372)/h、消除速率常數β=(0.083±0.067)/h、消除半衰期t1/2β=(9.418±7.256)h、藥-時麯線下麵積AUC0-12=(9.628±2.281)mg·h/L.結論 高效液相色譜法測定血漿中蘆丁濃度準確、簡便,適用于柿葉提取物中蘆丁的藥動學研究.
목적 건립고효액상색보법(high performance liquid chromatography,HPLC)측정시협제취물중호정재대서체내적혈약농도,병탐토기재대서체내약물대사동역학(이하간칭약동학)특정.방법 SD대서급여시협제취물관위,재불동시간점안후정맥총취혈,예처리후측정혈장중호정적농도,경3P97연건처리수거.결과 시협제취물중호정재대서체내적약동학부합이방실모형,호정재체내교쾌지피흡수,급약후1 h즉체도봉농도,봉농도Cmax위(1.658±0.184) mg/L,기타주요약동학삼수흡수속솔상수Ka=(2.436±0.883)/h、분포속솔상수α=(1.002±0.372)/h、소제속솔상수β=(0.083±0.067)/h、소제반쇠기t1/2β=(9.418±7.256)h、약-시곡선하면적AUC0-12=(9.628±2.281)mg·h/L.결론 고효액상색보법측정혈장중호정농도준학、간편,괄용우시협제취물중호정적약동학연구.
Objective To establish a high performance liquid chromatography (HPLC) method for measuring the concentration of rutin in extract of persimmon leaves in rats, and to study its pharmacokinetics.Methods Blood sample were collected through venous plexus in rat eye at different time after oral administration of the extract of persimmon leaves. After liquid extraction with methanol, the concentration of rutin in plasma was measured. Then pharmacokinetics parameters were calculated by 3P97 program.Results The results showed that plasma concentration-time curve of rutin in rats was coincided with two-compartment model, rutin was absorbed fast in rats. Peak concentration (Cmax) of rutin in rat plasma was (1.658±0.184) mg/L one hour after oral administration. The other main pharmacokinetic parameters were as follows: absorption rate constant (Ka) was (2.436±0.883)/h, distribution rate constant (α) was (1.002±0.372)/h, elimination rate constant (β) was (0.083±0.067)/h, elimination half time (t1/2β) was (9.418±7.256) h, area under the concentration-versus-time curve (AUC0-12) was (9.628±2.281) mg·h/L.Conclusions HPLC method is accurate and sensitive, it is suitable for the pharmacokinetic study of rutin in extract of persimmon leaves in rats.
