中华实用儿科临床杂志
中華實用兒科臨床雜誌
중화실용인과림상잡지
Journal of Applied Clinical Pediatrics
2015年
17期
1332-1335
,共4页
肥胖相关性肾病%转化生长因子-β1%肥胖
肥胖相關性腎病%轉化生長因子-β1%肥胖
비반상관성신병%전화생장인자-β1%비반
Obesity-related glomerulopathy%Transforming growth factor-β1%Obesity
目的 探讨转化生长因子-β1 (TGF-β1)与肥胖相关性肾病(ORG)的联系,及其在疾病发生发展过程中的作用和可能的治疗方案.方法 30只SD大鼠按体质量随机分为2组,对照组(15只)以正常饲料喂养,ORG模型组(15只)以高脂高能量饲料饲养,8周末将所有大鼠处死并取其双肾,行免疫组织化学定性检测肥胖大鼠肾脏TGF-β1的表达;实时荧光定量(RT)-PCR提取并检测TGF-β1 mRNA表达,进行半定量分析,采用Western blot检测TGF-β1蛋白表达情况.结果采用SPSS 13.0软件进行统计学处理.结果 与对照组比较,ORG模型组肾组织免疫组织化学结果显示TGF-β1定性表达增多,主要表达于肾小管及肾间质部位,对照组、OR模型组平均吸光度值分别为0.040±0.013、0.171 ±0.084,差异有统计学意义(P ＜0.05);RT-PCR结果提示ORG模型组TGF-β1 mRNA表达较对照组增多(4.4比0.6),差异有统计学意义(P＜0.05);Western blot检测ORG模型组TGF-β1蛋白表达水平高于对照组(4.3比0.4),差异有统计学意义(P=O.002).结论 ORG大鼠肾脏TGF-β1表达水平升高,该因子参与了ORG的疾病发展过程,为ORG的发病机制及可能的治疗提供依据.
目的 探討轉化生長因子-β1 (TGF-β1)與肥胖相關性腎病(ORG)的聯繫,及其在疾病髮生髮展過程中的作用和可能的治療方案.方法 30隻SD大鼠按體質量隨機分為2組,對照組(15隻)以正常飼料餵養,ORG模型組(15隻)以高脂高能量飼料飼養,8週末將所有大鼠處死併取其雙腎,行免疫組織化學定性檢測肥胖大鼠腎髒TGF-β1的錶達;實時熒光定量(RT)-PCR提取併檢測TGF-β1 mRNA錶達,進行半定量分析,採用Western blot檢測TGF-β1蛋白錶達情況.結果採用SPSS 13.0軟件進行統計學處理.結果 與對照組比較,ORG模型組腎組織免疫組織化學結果顯示TGF-β1定性錶達增多,主要錶達于腎小管及腎間質部位,對照組、OR模型組平均吸光度值分彆為0.040±0.013、0.171 ±0.084,差異有統計學意義(P ＜0.05);RT-PCR結果提示ORG模型組TGF-β1 mRNA錶達較對照組增多(4.4比0.6),差異有統計學意義(P＜0.05);Western blot檢測ORG模型組TGF-β1蛋白錶達水平高于對照組(4.3比0.4),差異有統計學意義(P=O.002).結論 ORG大鼠腎髒TGF-β1錶達水平升高,該因子參與瞭ORG的疾病髮展過程,為ORG的髮病機製及可能的治療提供依據.
목적 탐토전화생장인자-β1 (TGF-β1)여비반상관성신병(ORG)적련계,급기재질병발생발전과정중적작용화가능적치료방안.방법 30지SD대서안체질량수궤분위2조,대조조(15지)이정상사료위양,ORG모형조(15지)이고지고능량사료사양,8주말장소유대서처사병취기쌍신,행면역조직화학정성검측비반대서신장TGF-β1적표체;실시형광정량(RT)-PCR제취병검측TGF-β1 mRNA표체,진행반정량분석,채용Western blot검측TGF-β1단백표체정황.결과채용SPSS 13.0연건진행통계학처리.결과 여대조조비교,ORG모형조신조직면역조직화학결과현시TGF-β1정성표체증다,주요표체우신소관급신간질부위,대조조、OR모형조평균흡광도치분별위0.040±0.013、0.171 ±0.084,차이유통계학의의(P ＜0.05);RT-PCR결과제시ORG모형조TGF-β1 mRNA표체교대조조증다(4.4비0.6),차이유통계학의의(P＜0.05);Western blot검측ORG모형조TGF-β1단백표체수평고우대조조(4.3비0.4),차이유통계학의의(P=O.002).결론 ORG대서신장TGF-β1표체수평승고,해인자삼여료ORG적질병발전과정,위ORG적발병궤제급가능적치료제공의거.
Objective To explore the relationship between transforming growth factor-β1 (TGF-β1) and obesity-related glomerulopathy (ORG),and to analyze the possible mechanism for ORG and the new approach to its treatment.Methods Based on their body weight,30 SD rats were randomly divided into 2 groups : the normal control group (15 rats) fed with common food and the ORG model group (15 rats) fed with fat-enriched diets.The rats were sacrificed at the end of the 8th week,and their kidneys were taken out.Immunohistochemistry was used to detect TGF-β1 protein expression.Real time (RT)-polymerase chain reaction (PCR) was used to extract and detect the expression of TGF-β1 mRNA,and Western blot was applied to examine the expression of TGF-β1 protein.The findings were analyzed by using SPSS 13.0 software.Results Compared with the control group, qualitative TGF-β1 expression in ORG model group were significantly increased detected by immunohistochemistry mainly in renal tubules and interstitium.The average absorbance value of the control group and the model ORG group was 0.040-0.013,0.171 ± 0.084, respectively.The difference was statistically significant(P ＜ 0.05).The expression of TGF-β1 mRNA detected by RT-PCR was also increased compared with that of the control group(4.4 vs 0.6).The difference was statistically significant(P ＜ 0.05).The protein expression of TGF-β 1 examined by Western blot showed that it was more than that in the control group(4.3 vs 0.4).The difference between the control group and ORG model group was statistically significant(P =0.002).Conclusions The expression of TGF-β 1 in kidneys of ORG model rats increased, which not only indicates it can participate in ORG's occurrence and development, but also provide the basis to find out the mechanism and the approach to treatment.
