国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
International Journal of Laboratory Medicine
2015年
20期
2994-2996
,共3页
血培养%病原菌%检出率%血流感染
血培養%病原菌%檢齣率%血流感染
혈배양%병원균%검출솔%혈류감염
blood culture%pathogens%detection%bloodstream infection
目的：分析该院血培养检出病原菌的分布和探讨双份血培养法在血流感染诊断中的价值。方法采集疑似血流感染患者的血液标本共2033份，1425份标本采用单份血培养法，608份标本采用双份血培养法进行检测。标本置Bact／Alert3D全自动血液培养仪进行血培养，对血培养阳性标本进行统计学分析。结果血培养阳性的标本共251份，检出率为12．3％（251／2033）。其中，单份血培养法检出率为11．9％（169／1425）；双份血培养法检出率为13．5％（82／608）。血培养共检出216株病原菌（同一患者多次培养出同一病原菌计为1株）。其中，革兰阳性（G＋）球菌占65．7％（142／216），以凝固酶阴性葡萄球菌为主；肠杆菌科细菌占20．8％（45／216），以大肠埃希菌为主；革兰阴性（G －）非发酵菌占8．8％（19／216），以鲍曼不动杆菌为主；真菌占3．2％（7／216）。双份血培养法的病原菌检出率高于单份血培养法的检出率（ P＜0．05），单份血培养阳性和双份血单瓶阳性检出病原菌以凝固酶阴性葡萄球菌所占比例最大（＞40．0％），双份血培养双瓶阳性检出的病原菌以G－非发酵菌及真菌常见。结论双份血培养双瓶阳性与单份血培养阳性、双份血培养单瓶阳性检出的主要病原菌类型不同。双份血培养对提高病原菌检出率和污染菌鉴别有一定作用。
目的：分析該院血培養檢齣病原菌的分佈和探討雙份血培養法在血流感染診斷中的價值。方法採集疑似血流感染患者的血液標本共2033份，1425份標本採用單份血培養法，608份標本採用雙份血培養法進行檢測。標本置Bact／Alert3D全自動血液培養儀進行血培養，對血培養暘性標本進行統計學分析。結果血培養暘性的標本共251份，檢齣率為12．3％（251／2033）。其中，單份血培養法檢齣率為11．9％（169／1425）；雙份血培養法檢齣率為13．5％（82／608）。血培養共檢齣216株病原菌（同一患者多次培養齣同一病原菌計為1株）。其中，革蘭暘性（G＋）毬菌佔65．7％（142／216），以凝固酶陰性葡萄毬菌為主；腸桿菌科細菌佔20．8％（45／216），以大腸埃希菌為主；革蘭陰性（G －）非髮酵菌佔8．8％（19／216），以鮑曼不動桿菌為主；真菌佔3．2％（7／216）。雙份血培養法的病原菌檢齣率高于單份血培養法的檢齣率（ P＜0．05），單份血培養暘性和雙份血單瓶暘性檢齣病原菌以凝固酶陰性葡萄毬菌所佔比例最大（＞40．0％），雙份血培養雙瓶暘性檢齣的病原菌以G－非髮酵菌及真菌常見。結論雙份血培養雙瓶暘性與單份血培養暘性、雙份血培養單瓶暘性檢齣的主要病原菌類型不同。雙份血培養對提高病原菌檢齣率和汙染菌鑒彆有一定作用。
목적：분석해원혈배양검출병원균적분포화탐토쌍빈혈배양법재혈류감염진단중적개치。방법채집의사혈류감염환자적혈액표본공2033빈，1425빈표본채용단빈혈배양법，608빈표본채용쌍빈혈배양법진행검측。표본치Bact／Alert3D전자동혈액배양의진행혈배양，대혈배양양성표본진행통계학분석。결과혈배양양성적표본공251빈，검출솔위12．3％（251／2033）。기중，단빈혈배양법검출솔위11．9％（169／1425）；쌍빈혈배양법검출솔위13．5％（82／608）。혈배양공검출216주병원균（동일환자다차배양출동일병원균계위1주）。기중，혁란양성（G＋）구균점65．7％（142／216），이응고매음성포도구균위주；장간균과세균점20．8％（45／216），이대장애희균위주；혁란음성（G －）비발효균점8．8％（19／216），이포만불동간균위주；진균점3．2％（7／216）。쌍빈혈배양법적병원균검출솔고우단빈혈배양법적검출솔（ P＜0．05），단빈혈배양양성화쌍빈혈단병양성검출병원균이응고매음성포도구균소점비례최대（＞40．0％），쌍빈혈배양쌍병양성검출적병원균이G－비발효균급진균상견。결론쌍빈혈배양쌍병양성여단빈혈배양양성、쌍빈혈배양단병양성검출적주요병원균류형불동。쌍빈혈배양대제고병원균검출솔화오염균감별유일정작용。
Objective To investigate the distribution of pathogens detected in blood cultures and the value of double blood cul‐ture .Methods There were 2 033 cases of blood cultures including 1 425 cases of single blood culture and 608 cases of doube blood cultures were in the study .Blood cultures were performed by using Bact/Alert3D automated detection system ,and the positive blood culture results were statistically analyzed .Results There were 251 blood culture positive samples ,the detection rate was 12 .3% (251/2 033) .Among them ,the positive rate of single blood culture was 11 .9% (169/1 425);positive rate of double blood culture was 13 .5% (82/608) .There were 216 strains of pathogenic bacteria(repeatedly culture of the same pathogen from the same patient only accounted for 1 strain) .The isolates of gram positive(G+ ) cocci accounted for 65 .7% (142/216) and the major bacteria were coagulase negative Staphy lococci .Escherichia coli and Acinetobacter baumannii were the dominant isolates among Enterobac‐teriaceae[accounted for 20 .8% (45/216)] and Non‐fermenting gram‐negative(G-) rods[accounted for 8 .8% (19/216)] ,respective‐ly .The isolating rate of doube blood cultures was significantly higher than the single one(P<0 .05) .Coagulase‐negative staphylo‐coccus were isolated primarily in single blood culture and double blood culture with only one test postive .Non‐fermenting gram‐neg‐ative rods and fungi were the most common strains isolated from double blood culture with two tests both postive .Conclusion The distributions of pathogens isolated from single blood culture ,double blood culture with only one test positive and two tests positive are different .Because collecting double sets of blood cultures can significantly increase the isolating rates and distinguish contamina‐tion from real bloodstream infection ,collecting double sets of blood cultures should be recommended .Double blood culture is helpful to improve the detection rate of pathogenic bacteria and bacteria identification .
