CAJ | 학술논문

目的：研究纤维蛋白原（Fib）γ基因多态性位点FGG 9340T＞C（rs1049636）与血浆Fibγ′水平及缺血性脑卒中（IS）易感性的相关性。方法采用病例‐对照研究方法，患者组选取华东地区IS患者421例，男283例、女138例，年龄40～88岁，均为汉族，无亲缘关系；选取性别、年龄与患者组相匹配的个体421例作为对照组；应用双抗夹心酶联免疫吸附测定（ELISA ）检测两组人群血浆Fibγ′水平。应用聚合酶链式反应‐连接酶检测反应（PCR‐LDR）的方法检测rs1049636位点基因型。结果患者组血浆Fibγ′水平[（159．4±97．4）U／dL]明显高于对照组[（114．2±73．0）U／dL]，差异有统计学意义（P＜0．001）；不论是在患者组还是在对照组，Fib基因rs1049636 C等位基因均与高水平的血浆Fibγ′相关；但rs1049636各基因型及等位基因的分布在患者组和对照组间比较，差异均无统计学意义（P＝0．077，0．106）。结论血浆Fibγ′可以增加中国人群IS的易感性，FGG基因多态性位点rs1049636 C等位基因虽与血浆Fibγ′水平相关，但可能与中国人群IS的发病无关。
목적：연구섬유단백원（Fib）γ기인다태성위점FGG 9340T＞C（rs1049636）여혈장Fibγ′수평급결혈성뇌졸중（IS）역감성적상관성。방법채용병례‐대조연구방법，환자조선취화동지구IS환자421례，남283례、녀138례，년령40～88세，균위한족，무친연관계；선취성별、년령여환자조상필배적개체421례작위대조조；응용쌍항협심매련면역흡부측정（ELISA ）검측량조인군혈장Fibγ′수평。응용취합매련식반응‐련접매검측반응（PCR‐LDR）적방법검측rs1049636위점기인형。결과환자조혈장Fibγ′수평[（159．4±97．4）U／dL]명현고우대조조[（114．2±73．0）U／dL]，차이유통계학의의（P＜0．001）；불론시재환자조환시재대조조，Fib기인rs1049636 C등위기인균여고수평적혈장Fibγ′상관；단rs1049636각기인형급등위기인적분포재환자조화대조조간비교，차이균무통계학의의（P＝0．077，0．106）。결론혈장Fibγ′가이증가중국인군IS적역감성，FGG기인다태성위점rs1049636 C등위기인수여혈장Fibγ′수평상관，단가능여중국인군IS적발병무관。
Objective To explore the association between fibrinogen gene polymorphism(rs1049636) and serum γ′ fibrinogen level and ischemic stroke (IS) .Methods 421 IS patients and 421 age‐and gender‐ matched healthy controls ,including 283 males and 138 females ,were recruited in this assay .The plasma γ′fibrinogen concentration was measured by enzyme‐linked immunosor‐bent assay (ELISA) .Fibrinogen gene polymorphism(rs1049636) were genotyped by using PCR‐LDR assay .Results γ′fibrinogen concentrations in IS patients[(159 .4 ± 97 .4)U/dL] were significantly higher than that in control group[(114 .2 ± 73 .0)U/dL] with statistically significant difference(P<0 .001) .Single nucleotide polymorphism(SNP) analysis showed that rs1049636 C allele was significantly associated withγ′fibrinogen level ,but not associated with increased risk of IS(P=0 .077) .Conclusion An associ‐ation between increasedγ′fibrinogen level and IS existed in Chinese Han population .However ,no association between rs1049636 C allele and IS risk was observed in our study .