CAJ | 학술논문

目的：建立一种新型电化学免疫传感器用于赭曲霉毒素A（OTA）的快速检测。方法以固载于玻碳电极（GCE）表面的单壁碳纳米管／壳聚糖（SWNTs／CS）复合物膜为基底构建OTA双层自组装免疫传感器，采用电镜扫描分析、方波伏安和循环伏安法对传感器进行表征分析，并研究其用于检测的特异性。结果壳聚糖／单壁碳纳米管复合膜能够显著增加电化学免疫传感器检测OTA的灵敏度，能够有效地分辨不同种类的真菌毒素。结论本研究构建的电化学免疫传感器检测OTA具有快速、简便、检出限低，特异性强等特点。
목적：건립일충신형전화학면역전감기용우자곡매독소A（OTA）적쾌속검측。방법이고재우파탄전겁（GCE）표면적단벽탄납미관／각취당（SWNTs／CS）복합물막위기저구건OTA쌍층자조장면역전감기，채용전경소묘분석、방파복안화순배복안법대전감기진행표정분석，병연구기용우검측적특이성。결과각취당／단벽탄납미관복합막능구현저증가전화학면역전감기검측OTA적령민도，능구유효지분변불동충류적진균독소。결론본연구구건적전화학면역전감기검측OTA구유쾌속、간편、검출한저，특이성강등특점。
Objective To develop a new type of electrochemical immunosensor for the detection of ochratoxin A (OTA ) . Methods Double layers of self‐assembly immunosensor for the detection of OTA were constructed based on the composite single‐walled carbon nanotubes(SWNTs)/chitosan(CS) membrane immobilized on glassy carbon electrode(GC) .Scanning electron mi‐croscopy(SEM) ,square wave voltammetry and cyclic voltammetry were used to analyze the characterization of the sensor ,then its specificity for detection was studied .Results SWNTs/CS composit membrane could increase the sensitivity of OTA detection sig‐nificantly ,and effectively distinguish the different types of mycotoxins .Conclusion The electrochemical immunosensor developed in the study is easy to operate and could detect OTA rapidly with good specificity and low detection limit .