CAJ | 학술논문

目的：研究高尔基体蛋白73（GP73）联合甲胎蛋白（AFP）、α‐L岩藻糖苷酶（AFU）、α‐抗胰蛋白酶（α‐AT）检测对原发性肝癌诊断的价值。方法选取2011年10月至2013年5月在该院住院及就诊的原发性肝癌、肝炎和肝硬化患者及健康体检者各100例作为研究对象，使用Beckman DxI800化学发光分析仪检测血清AFP水平；用Beckman DxC800全自动生化分析仪检测血清AFU、α‐AT水平；用GP73酶联免疫定量检测试剂盒测定 GP73水平。结果原发性肝癌患者、肝炎和肝硬化患者血清GP73、AFP、AFU、α‐AT水平明显高于健康者（P＜0．05）。原发性肝癌患者血清GP73、AFP、AFU、α‐AT 水平明显高于肝炎和肝硬化患者（P＜0．05）；血清GP73、AFP、AFU、α‐AT水平单一检测时的灵敏度分别为75％、72％、23％、67％，特异度分别为95％、95％、100％、97％。4种肿瘤标志物联合检测的灵敏度和特异度分别为99％和100％。联合检测的灵敏度与单项检测比较，差异有统计学意义（P＜0．05）。结论 GP73联合AFP、AFU、α‐AT检测可以提高原发性肝癌的诊断效能。
목적：연구고이기체단백73（GP73）연합갑태단백（AFP）、α‐L암조당감매（AFU）、α‐항이단백매（α‐AT）검측대원발성간암진단적개치。방법선취2011년10월지2013년5월재해원주원급취진적원발성간암、간염화간경화환자급건강체검자각100례작위연구대상，사용Beckman DxI800화학발광분석의검측혈청AFP수평；용Beckman DxC800전자동생화분석의검측혈청AFU、α‐AT수평；용GP73매련면역정량검측시제합측정 GP73수평。결과원발성간암환자、간염화간경화환자혈청GP73、AFP、AFU、α‐AT수평명현고우건강자（P＜0．05）。원발성간암환자혈청GP73、AFP、AFU、α‐AT 수평명현고우간염화간경화환자（P＜0．05）；혈청GP73、AFP、AFU、α‐AT수평단일검측시적령민도분별위75％、72％、23％、67％，특이도분별위95％、95％、100％、97％。4충종류표지물연합검측적령민도화특이도분별위99％화100％。연합검측적령민도여단항검측비교，차이유통계학의의（P＜0．05）。결론 GP73연합AFP、AFU、α‐AT검측가이제고원발성간암적진단효능。
Objective To study the diagnostic value of the golgi protein 73 (GP73) combined with alpha‐fetoprotein (AFP) ,α‐L‐fucosidase (AFU) andα‐antitrypsin(α‐AT) detection in primary hepatic carcinoma(PHC) .Methods 100 patients with PHC ,100 patients with hepatitis and cirrhosis ,and 100 healthy people hospitalized in the hospital from October 2011 to May 2013 were en‐rolled in the study .Serum AFP levels were detected by using Beckman DxI800 chemiluminescent analyzer ,serum AFU ,α‐AT levels were detected by using Beckman DxC800 automatic biochemical analyzer ,and GP73 levels were measured by using enzyme‐linked immunosorbent GP73 quantitative detection kits .Results In patients with PHC and patients with hepatitis and cirrhosis ,serum GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than healthy people(P<0 .05) .In patients with PHC ,GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than those in patients with hepatitis and cirrhosis(P<0 .05) .The sensitivities of single serum GP73 ,AFP ,AFU ,α‐AT detection were 75% ,72% ,23% ,67% respectively and specificity were 95% ,95% ,100% ,97% respective‐ly .The sensitivity and specificity of the 4 tumor markers′combined detection were 99% and 100% .The sensitivity of combined de‐tection was significantly different from single detection(P<0 .05) .Conclusion GP73 ,AFP ,AFU ,α‐AT may improve the diagnostic efficiency of primary liver cancer .