农业环境科学学报
農業環境科學學報
농업배경과학학보
Journal of Agro-Environment Science
2015年
10期
1936-1942
,共7页
袁敏%唐美珍%罗彦鹤%常文韬%闫佩%宋兵魁%邢志杰%赵晶磊%廖光龙
袁敏%唐美珍%囉彥鶴%常文韜%閆珮%宋兵魁%邢誌傑%趙晶磊%廖光龍
원민%당미진%라언학%상문도%염패%송병괴%형지걸%조정뢰%료광룡
盐胁迫%甲维盐·毒死蜱%土壤微生物%土壤酶活性%正交实验
鹽脅迫%甲維鹽·毒死蜱%土壤微生物%土壤酶活性%正交實驗
염협박%갑유염·독사비%토양미생물%토양매활성%정교실험
salt stress%emamectin benzoate-chlorpyrifos mix%soil microbe%soil enzyme activity%orthogonal experimental design
采用实验室盆栽方法,运用正交试验探究盐与复配农药甲维盐·毒死蜱的复合污染对菜地土壤中酶活性与微生物数量的影响。结果表明:盐胁迫和甲维盐·毒死蜱分别作用下土壤中的脲酶、蔗糖酶、脱氢酶和过氧化氢酶活性均受到不同程度的抑制作用,且随着盐分水平和甲维盐·毒死蜱浓度的升高呈显著下降趋势;土壤细菌、真菌和放线菌数量亦随土壤盐分和甲维盐·毒死蜱浓度的增加而减少。随着时间的延长,农药在土壤中不断被降解而减少,对微生物的毒害性也就随着减弱,并最终恢复到与对照组相一致的水平。但是在与高盐复合胁迫时,土壤微生物数量与土壤酶活性始终处于被抑制状态,由此可以说明盐胁迫是抑制土壤微生物数量和酶活性的主导因素。
採用實驗室盆栽方法,運用正交試驗探究鹽與複配農藥甲維鹽·毒死蜱的複閤汙染對菜地土壤中酶活性與微生物數量的影響。結果錶明:鹽脅迫和甲維鹽·毒死蜱分彆作用下土壤中的脲酶、蔗糖酶、脫氫酶和過氧化氫酶活性均受到不同程度的抑製作用,且隨著鹽分水平和甲維鹽·毒死蜱濃度的升高呈顯著下降趨勢;土壤細菌、真菌和放線菌數量亦隨土壤鹽分和甲維鹽·毒死蜱濃度的增加而減少。隨著時間的延長,農藥在土壤中不斷被降解而減少,對微生物的毒害性也就隨著減弱,併最終恢複到與對照組相一緻的水平。但是在與高鹽複閤脅迫時,土壤微生物數量與土壤酶活性始終處于被抑製狀態,由此可以說明鹽脅迫是抑製土壤微生物數量和酶活性的主導因素。
채용실험실분재방법,운용정교시험탐구염여복배농약갑유염·독사비적복합오염대채지토양중매활성여미생물수량적영향。결과표명:염협박화갑유염·독사비분별작용하토양중적뇨매、자당매、탈경매화과양화경매활성균수도불동정도적억제작용,차수착염분수평화갑유염·독사비농도적승고정현저하강추세;토양세균、진균화방선균수량역수토양염분화갑유염·독사비농도적증가이감소。수착시간적연장,농약재토양중불단피강해이감소,대미생물적독해성야취수착감약,병최종회복도여대조조상일치적수평。단시재여고염복합협박시,토양미생물수량여토양매활성시종처우피억제상태,유차가이설명염협박시억제토양미생물수량화매활성적주도인소。
Soil microorganisms and enzyme activities are important indicators of soil ecosystem responses to environmental stresses. The im-pacts of salt and emamectin benzoate-chlorpyrifos mix on soil enzyme activities and microorganism population in a vegetable soil was exam-ined using the orthogonal experimental design and pot culture. Results showed that the activities of soil urease, sucrase, dehydrogenase and catalase were inhibited in varying degrees by salt and emamectin benzoate-chlorpyrifos mix in a dose-effect mode. Similarly, the populations of soil bacteria, fungi, and actinomycetes decreased with increasing stresses. As time passed, the pesticide degraded gradually, thus reducing its toxicity and restoring the soil microbes to the control level. However, soil enzyme activities and microorganism quantities were inhibited all the time under co-existence of salt and pesticide. These results suggest that salt stress is the controlling factor of soil enzyme activity and mi-croorganism inhibition by pesticides.