CAJ | 학술논문

为了解磷高效转基因水稻对土壤无机磷和有机磷形态特征及磷酸酶活性的影响，以两种磷高效转基因水稻OsPT4（磷高效吸收材料）和PHO2（磷高效突变体材料）及其非转基因亲本水稻日本晴为供试材料，采用盆栽试验研究磷高效水稻的种植对根际土壤磷素形态及其有效性的影响。结果表明，水稻OsPT4和PHO2的根干重、生物量和磷素积累量均有不同程度的增加，磷高效转基因水稻OsPT4分蘖期和扬花期根际土壤的有效磷浓度与亲本差异显著；在四个生育期内，磷高效转基因水稻OsPT4和磷高效突变体PHO2根际土壤各个磷形态的含量变化趋势与其亲本日本晴基本一致，OsPT4和PHO2水稻根际土壤无机磷组分含量表现为Ca10-P﹥O-P﹥Ca8-P﹥Fe-P﹥Al-P﹥Ca2-P，有机磷浓度表现为中活性有机磷﹥中稳性有机磷﹥高稳性有机磷﹥活性有机磷，且各时期根际土壤的不同磷形态含量和同期日本晴相比均差异不显著。磷高效转基因水稻材料OsPT4、磷高效突变体水稻材料PHO2根际土壤磷酸酶活性和同期日本晴相比均差异不显著。研究表明磷高效转基因水稻材料OsPT4和磷高效突变体材料PHO2一年期种植对根际土壤的磷素形态和磷酸酶活性的影响不显著。
위료해린고효전기인수도대토양무궤린화유궤린형태특정급린산매활성적영향，이량충린고효전기인수도OsPT4（린고효흡수재료）화PHO2（린고효돌변체재료）급기비전기인친본수도일본청위공시재료，채용분재시험연구린고효수도적충식대근제토양린소형태급기유효성적영향。결과표명，수도OsPT4화PHO2적근간중、생물량화린소적루량균유불동정도적증가，린고효전기인수도OsPT4분얼기화양화기근제토양적유효린농도여친본차이현저；재사개생육기내，린고효전기인수도OsPT4화린고효돌변체PHO2근제토양각개린형태적함량변화추세여기친본일본청기본일치，OsPT4화PHO2수도근제토양무궤린조분함량표현위Ca10-P﹥O-P﹥Ca8-P﹥Fe-P﹥Al-P﹥Ca2-P，유궤린농도표현위중활성유궤린﹥중은성유궤린﹥고은성유궤린﹥활성유궤린，차각시기근제토양적불동린형태함량화동기일본청상비균차이불현저。린고효전기인수도재료OsPT4、린고효돌변체수도재료PHO2근제토양린산매활성화동기일본청상비균차이불현저。연구표명린고효전기인수도재료OsPT4화린고효돌변체재료PHO2일년기충식대근제토양적린소형태화린산매활성적영향불현저。
High-phosphorus(P)efficiency rice has great potential to take up phosphorus from soil, which may influence the status of soil phosphorus fractions. We designed an experiment to identify the characteristics of phosphorus fractions in rhizospheric soil of a high-P effi-ciency transgenic rice during its growth period using japonica rice cultivar Nipponbare(CK)and its high- P efficiency transgenic rice (OsPT4)and high- P efficiency mutant(PHO2)as model plants. Alkaline phosphatase activity in rhizospheric soil was also examined. Significant higher root dry weight, biomass and P accumulation were observed in OsPT4 and PHO2 than in CK. PHO2 also had higher phos-phorus use efficiency than Nipponbare did. Compared with the control, available phosphorus concentrations in rhizospheric soil of OsPT4 was significantly higher at tillering stage, whereas it was significantly lower at heading stage. Phosphorus fractions in OsPT4 and PHO2 rhi-zospheric soils had no significant difference from those of Nipponbare during their growth period. Inorganic P fractions were in order:Ca10-P﹥O-P﹥Ca8-P﹥Fe-P﹥Al-P﹥Ca2-P. Of organic P fractions, moderate labile organic phosphorus was dominant, followed by resistant organic phosphorus and moderate-resistant organic phosphorus, while labile organic phosphorus was the lowest. No significant difference was ob-served in the activity of soil alkaline phosphatase between OsPT4 and PHO2 and Nipponbare during their growth period. High-P efficiency rice OsPT4 and PHO2 had no significant effects on phosphorus fractions and alkaline phosphatase activity in rhizospheric soil in the first year.