CAJ | 학술논문

以猪粪为原料，采用批式试验方法，研究不同氨氮添加量（0、400、800、1600、2400、3200、4000 mg·L-1）对厌氧消化产气效果的影响。结果表明：随着氨氮添加量的增加，总产气量和CH4产率均呈现先升高后降低的变化趋势，氨氮添加量≥2400 mg·L-1时，厌氧消化过程受到显著抑制；不同处理中猪粪挥发性固体（VS）的CH4产率分别为328.5、338.1、323.2、304.9、276.2、124.9、56.1 mL· g-1。氨氮添加量为0~800 mg·L-1时，最大VS产CH4速率分别为18.3、18.4、17.1 mL·g-1·d-1；氨氮添加量为2400 mg·L-1时，产气高峰推迟，产CH4速率明显降低；氨氮添加量≥400 mg·L-1时，厌氧消化30 d底物的生物转化产CH4效率随氨氮添加量的增加逐渐降低，分别为56.7％、54.5％、52.4％、30.6％、1.6％和1.3％；氨氮添加量为400~2400 mg·L-1时，乙酸利用型产甲烷菌Methanosaeta的相对丰度总体随氨氮质量浓度的增加而降低，而氢利用型产甲烷菌Methanosarcina和Methanococcus具有相反的变化规律。
이저분위원료，채용비식시험방법，연구불동안담첨가량（0、400、800、1600、2400、3200、4000 mg·L-1）대염양소화산기효과적영향。결과표명：수착안담첨가량적증가，총산기량화CH4산솔균정현선승고후강저적변화추세，안담첨가량≥2400 mg·L-1시，염양소화과정수도현저억제；불동처리중저분휘발성고체（VS）적CH4산솔분별위328.5、338.1、323.2、304.9、276.2、124.9、56.1 mL· g-1。안담첨가량위0~800 mg·L-1시，최대VS산CH4속솔분별위18.3、18.4、17.1 mL·g-1·d-1；안담첨가량위2400 mg·L-1시，산기고봉추지，산CH4속솔명현강저；안담첨가량≥400 mg·L-1시，염양소화30 d저물적생물전화산CH4효솔수안담첨가량적증가축점강저，분별위56.7％、54.5％、52.4％、30.6％、1.6％화1.3％；안담첨가량위400~2400 mg·L-1시，을산이용형산갑완균Methanosaeta적상대봉도총체수안담질량농도적증가이강저，이경이용형산갑완균Methanosarcina화Methanococcus구유상반적변화규률。
Anaerobic digestion is a commonly used technique for treating livestock manure and producing biogas. However, ammonia gener-ated during the digestion may reduce the efficiency of an anaerobic digestion system. Here a batch experiment was carried out to investigate the effect of ammonia on anaerobic digestion of swine manure by adding ammonia at 0, 400, 800, 1600, 2400, 3200 mg·L-1 and 4000 mg·L-1 under mesophilic conditions(35℃). Cumulative biogas production had no obvious differences at 0~800 mg·L-1 of ammonia concentrations. However, the lag phase appeared and cumulative biogas decreased when ammonia was higher than 2400 mg·L-1. Yields of CH4 were 328.5, 338.1, 323.2, 304.9, 276.2, 124.9 mL·g-1 and 56.1 mL·g-1 VS for 0, 400, 800, 1600, 2400, 3200 mg N·L-1 and 4000 mg N·L-1, respective-ly. The maximum CH4 yield rates were 18.3, 18.4, 17.1 mL·g-1·d-1 at 0, 400 mg N·L-1, and 800 mg N·L-1 of ammonia added, respectively. Increasing ammonia from 3200 mg·L-1 to 4000 mg·L-1 sharply decreased the CH4 yield rates and greatly delayed the peaks. The biodegrad-ability(BAD)of VS at 30 d was 55.6%, 56.7%, 54.5%, 52.4%, 30.6%, 1.6%and 1.3%for 0, 400, 800, 1600, 2400, 3200 mg N·L-1 and 4000 mg N·L-1, respectively. The results from terminal-restriction fragment length polymorphism(T-RFLP)and clone library showed that the relative abundance of acetate-utilizing Methanosaeta decreased with increases in ammonia concentrations, but the abundance of hydro-gen-utilizing Methanosarcina and Methanococcus had an opposite tendency at ammonia range of 400~2400 mg·L-1.