CAJ | 학술논문

为了给评价禽舍内生物气溶胶的危害提供基础数据，对笼养和网上养殖两种方式下，集约化养殖鸡舍内细菌气溶胶的浓度进行检测，并利用变性梯度凝胶电泳（PCR-DGGE）对空气中全部细菌的群落结构进行对比分析。结果显示：笼养舍内细菌气溶胶浓度和生物多样性均高于网上养殖，相同养殖方式下，生物多样性随着鸡龄的增加而呈现减小趋势；鸡舍空气环境中优势细菌主要为拟杆菌门（Bacteroidetes）、变形菌门（Proteobacteria）和厚壁菌门（Firmicutes），其中笼养和网上养殖舍内细菌气溶胶的优势菌属分别为Enterobacter和Escherichia。
위료급평개금사내생물기용효적위해제공기출수거，대롱양화망상양식량충방식하，집약화양식계사내세균기용효적농도진행검측，병이용변성제도응효전영（PCR-DGGE）대공기중전부세균적군락결구진행대비분석。결과현시：롱양사내세균기용효농도화생물다양성균고우망상양식，상동양식방식하，생물다양성수착계령적증가이정현감소추세；계사공기배경중우세세균주요위의간균문（Bacteroidetes）、변형균문（Proteobacteria）화후벽균문（Firmicutes），기중롱양화망상양식사내세균기용효적우세균속분별위Enterobacter화Escherichia。
Bioaerosols emitted from confined animal feeding operations may pose health risks to workers and animals. In this study, content of culturable airborne bacteria was detected in the atmospheric environments of both layer and broiler houses. In addition, corresponding mi-crobial communities were analyzed using denatured gradient gel electrophoresis(DGGE). Results indicated that both content and microbial diversity of airborne bacteria in the layer houses were higher than those in the broiler ones. Under the same farming mode, microbial diversi-ty decreased with increasing age of poultry. The main bacteria in atmospheric environment of confined poultry houses were Bacteroidetes, Proteobacteria and Firmicutes. Enterobacter and Escherichia were the predominant genus of airborne bacteria in layer and broiler houses, re-spectively.