CAJ | 학술논문

研究制备了海藻酸钙/明胶微胶珠，并探讨了此种微胶珠的机械和渗透性能及作为细胞载体用于神经干细胞体外培养的可行性。使用自制穿刺力测试仪检测不同浓度的海藻酸钠、明胶和 CaCl2对微胶珠机械强度的影响；采用DMEM(Dulbecco's Modified Eagle Media)培养基浸泡法测试微胶珠的耐浸泡能力；试验以微胶珠为对象建立扩散模型以研究葡萄糖和牛血清蛋白(Bovine serum albumin BSA)在微胶珠中的扩散情况，并模拟得扩散系数与物质浓度之间的关系，此后，通过包埋培养昆明小鼠神经干细胞探索微胶珠用于神经干细胞体外培养的可行性。通过综合分析强度和扩散性能以及培养细胞的状况等，得出海藻酸钙/明胶微胶珠的最佳制备参数：海藻酸钠1.5%(wt)、明胶0.5%(wt)、CaCl24%(wt)，并发现所研究制备的海藻酸钙/明胶微胶珠具有良好的细胞相容性，适于作为细胞载体用于神经干细胞的体外培养。
연구제비료해조산개/명효미효주，병탐토료차충미효주적궤계화삼투성능급작위세포재체용우신경간세포체외배양적가행성。사용자제천자력측시의검측불동농도적해조산납、명효화 CaCl2대미효주궤계강도적영향；채용DMEM(Dulbecco's Modified Eagle Media)배양기침포법측시미효주적내침포능력；시험이미효주위대상건립확산모형이연구포도당화우혈청단백(Bovine serum albumin BSA)재미효주중적확산정황，병모의득확산계수여물질농도지간적관계，차후，통과포매배양곤명소서신경간세포탐색미효주용우신경간세포체외배양적가행성。통과종합분석강도화확산성능이급배양세포적상황등，득출해조산개/명효미효주적최가제비삼수：해조산납1.5%(wt)、명효0.5%(wt)、CaCl24%(wt)，병발현소연구제비적해조산개/명효미효주구유량호적세포상용성，괄우작위세포재체용우신경간세포적체외배양。
Ca-alginate/gelatin (CAG) microbeads were prepared and their mechanical strength, permeability and feasibility forin vitroculture of neural stem cells were investigated. The effects of sodium alginate, gelatin and calcium chloride concentrations on the mechanical strength of the CAG microbeads were determined using a self-made puncture force tester. Their decay resistance was tested via DMEM (Dulbecco's Modified Eagle Media). A diffusion model was built to study the diffusion of glucose and bovine serum albumin(BSA) in the microbeads with diffusion coefficient calculated. The feasibility ofin vitro culture for neural stem cells from Kunming mouse was also studied. The preparation conditions were optimized and the results show that microbeads prepared with 1.5% (wt) sodium alginate, 0.5% (wt) gelatin and 4% (wt) CaCl2can have the best cell culture performance. This experiment demonstrates that the microbeads prepared have good cytocompatibility for culturing neural stem cells (NSCs).