中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
10期
2520-2522
,共3页
程维刚%李蕾蕾%刘九洲%殷德涛%郭万里
程維剛%李蕾蕾%劉九洲%慇德濤%郭萬裏
정유강%리뢰뢰%류구주%은덕도%곽만리
乳腺癌%S期激酶相关蛋白2%p27kip1
乳腺癌%S期激酶相關蛋白2%p27kip1
유선암%S기격매상관단백2%p27kip1
Breast cancer%S-phase kinase-associated protein 2%p27kip1
目的 探讨浸润性乳腺癌组织中S期激酶相关蛋白2(Skp2)和细胞周期抑制蛋白p27kip1的免疫表达及其意义,同时探讨两者与临床病理特征的关系.方法 应用免疫组织化学(PV-6000)二步法检测40例乳腺癌组织中Skp2和p27kip1蛋白的表达,探讨其表达水平与临床病理特征的关系,以20例正常乳腺组织作为对照.结果 乳腺癌组织中Skp2蛋白表达水平与组织学分级(P＜0.01)、淋巴结转移度呈正相关(P＜0.01);p27kip1蛋白表达水平与组织学分级(P＜0.05)、淋巴结转移度呈负相关(P＜0.05).两者的表达与乳腺癌患者的年龄、绝经状况、肿块大小以及病理学分期无明显相关(P＞0.01).且Skp2蛋白与p27kip1蛋白的表达在乳腺癌组织中呈负相关(P＜0.01).结论 乳腺癌组织中Skp2蛋白高表达、p27kip1蛋白低表达参与了乳腺癌的发生、发展过程;联合检测两指标在蛋白水平的表达,有助于判断乳腺癌预后.
目的 探討浸潤性乳腺癌組織中S期激酶相關蛋白2(Skp2)和細胞週期抑製蛋白p27kip1的免疫錶達及其意義,同時探討兩者與臨床病理特徵的關繫.方法 應用免疫組織化學(PV-6000)二步法檢測40例乳腺癌組織中Skp2和p27kip1蛋白的錶達,探討其錶達水平與臨床病理特徵的關繫,以20例正常乳腺組織作為對照.結果 乳腺癌組織中Skp2蛋白錶達水平與組織學分級(P＜0.01)、淋巴結轉移度呈正相關(P＜0.01);p27kip1蛋白錶達水平與組織學分級(P＜0.05)、淋巴結轉移度呈負相關(P＜0.05).兩者的錶達與乳腺癌患者的年齡、絕經狀況、腫塊大小以及病理學分期無明顯相關(P＞0.01).且Skp2蛋白與p27kip1蛋白的錶達在乳腺癌組織中呈負相關(P＜0.01).結論 乳腺癌組織中Skp2蛋白高錶達、p27kip1蛋白低錶達參與瞭乳腺癌的髮生、髮展過程;聯閤檢測兩指標在蛋白水平的錶達,有助于判斷乳腺癌預後.
목적 탐토침윤성유선암조직중S기격매상관단백2(Skp2)화세포주기억제단백p27kip1적면역표체급기의의,동시탐토량자여림상병리특정적관계.방법 응용면역조직화학(PV-6000)이보법검측40례유선암조직중Skp2화p27kip1단백적표체,탐토기표체수평여림상병리특정적관계,이20례정상유선조직작위대조.결과 유선암조직중Skp2단백표체수평여조직학분급(P＜0.01)、림파결전이도정정상관(P＜0.01);p27kip1단백표체수평여조직학분급(P＜0.05)、림파결전이도정부상관(P＜0.05).량자적표체여유선암환자적년령、절경상황、종괴대소이급병이학분기무명현상관(P＞0.01).차Skp2단백여p27kip1단백적표체재유선암조직중정부상관(P＜0.01).결론 유선암조직중Skp2단백고표체、p27kip1단백저표체삼여료유선암적발생、발전과정;연합검측량지표재단백수평적표체,유조우판단유선암예후.
Objective To explore the immune expression of S-phase kinase-associated protein 2 (Skp2) and p27kip1 protein in invasive breascancetissue and itsignificance, and discussetheirelationship with clinicopathological factors.MethodImmunohistochemical (PV-6000) two-step method waused to detecthe Skp2 and p27kip1 protein expression in 40 caseof breascancer, and the relationship between the expression levelof Skp2 and p27kip1 proteinand clinicopathologifactorwaanalyzed.Twenty caseof normal breastissue served acontrols.ResultSkp2 protein expression levelin breascancetissue were positively correlated with histological grade (P ＜ 0.01), lymph node metastasi(P ＜ 0.01).The p27kip1 protein expression levelwere negatively correlated with histological grade (P ＜ 0.05), and lymph node metastasi(P ＜ 0.05).The expression levelof Skp2 and p27kip1 proteinwere nocorrelated with patients' age, tumosize and surgical pathological stage.Skp2 and p27kipl proteinin breascancetissue were negatively correlated.Conclusion High Skp2 protein expression in breascancetissue and low p27kip1 protein expression in breascanceoccurrence are involved in the developmenand process.Combined detection of two indicatorin the expression of protein levelhelpto judge the prognosiof breascancer.
