CAJ | 학술논문

目的研究不同形式的运动训练(自主运动、强迫运动和功能性电刺激诱导的运动)对血管性痴呆(VD)大鼠学习记忆功能的恢复及前额叶皮质区突触素(SYP)、突触后致密物-95(PSD-95)表达的影响.方法选择Wistar雄性大鼠40只,按随机数字表法分为模型组、假手术组、自主运动组、强迫运动组和功能性电刺激组,每组8只.采用双侧颈总动脉永久性结扎的方法制作VD模型,假手术组大鼠麻醉但不阻断颈总动脉.造模成功1周后,自主运动组大鼠在跑轮上自由运动,每日270 m;强迫运动组在电动跑轮上行强迫运动,每日270 m;功能性电刺激组采用功能性电刺激模拟大鼠前肢在跑轮上行走时的动作,按9 m/min的速度每日运动3次,每次10 min;模型组和假手术组造模成功后置于笼中自由活动.5组大鼠均于造模成功3周后,采用新奇事物识别实验测试大鼠学习记忆能力,测试后即刻取大鼠前额叶皮质采用Western blot技术检测上述各组突触素、突触后致密物-95的表达.结果自主运动组、强迫运动组和功能性电刺激组大鼠新奇事物认知指数分别为(0.65±0.15)、(0.59±0.12)和(0.62±0.14),较模型组的(0.45±0.13)均有明显升高,且差异有统计学意义(P＜0.05).自主运动组、强迫运动组和功能性电刺激组大鼠前额叶皮质区PSD-95表达水平分别为(1.01±0.05)、(0.95±0.15)和(1.06±0.09),较模型组的(0.58±0.15)均有明显升高,差异有统计学意义(P＜0.05).结论自主运动、强迫运动和功能性电刺激诱导的运动均可改善VD大鼠的学习记忆能力,其可能机制是自主运动、强迫运动和功能性电刺激诱导的运动均可促进前额叶皮质区突触后致密物-95的表达,但对突触素的表达影响不大.
목적 연구불동형식적운동훈련(자주운동、강박운동화공능성전자격유도적운동)대혈관성치태(VD)대서학습기억공능적회복급전액협피질구돌촉소(SYP)、돌촉후치밀물-95(PSD-95)표체적영향.방법 선택Wistar웅성대서40지,안수궤수자표법분위모형조、가수술조、자주운동조、강박운동조화공능성전자격조,매조8지.채용쌍측경총동맥영구성결찰적방법제작VD모형,가수술조대서마취단불조단경총동맥.조모성공1주후,자주운동조대서재포륜상자유운동,매일270 m;강박운동조재전동포륜상행강박운동,매일270 m;공능성전자격조채용공능성전자격모의대서전지재포륜상행주시적동작,안9 m/min적속도매일운동3차,매차10 min;모형조화가수술조조모성공후치우롱중자유활동.5조대서균우조모성공3주후,채용신기사물식별실험측시대서학습기억능력,측시후즉각취대서전액협피질채용Western blot기술검측상술각조돌촉소、돌촉후치밀물-95적표체.결과 자주운동조、강박운동조화공능성전자격조대서신기사물인지지수분별위(0.65±0.15)、(0.59±0.12)화(0.62±0.14),교모형조적(0.45±0.13)균유명현승고,차차이유통계학의의(P＜0.05).자주운동조、강박운동조화공능성전자격조대서전액협피질구PSD-95표체수평분별위(1.01±0.05)、(0.95±0.15)화(1.06±0.09),교모형조적(0.58±0.15)균유명현승고,차이유통계학의의(P＜0.05).결론 자주운동、강박운동화공능성전자격유도적운동균가개선VD대서적학습기억능력,기가능궤제시자주운동、강박운동화공능성전자격유도적운동균가촉진전액협피질구돌촉후치밀물-95적표체,단대돌촉소적표체영향불대.
Objective To study the effects of different types of exercise training on learning and memory, as well as on the expression of synaptophysin (SYP) and on postsynaptic density protein 95 (PSD-95) in rats in which a model of vascular dementia had been created.Methods Forty male Wistar rats were divided randomly into a voluntary exercise group (V-EX) , a forced exercise group (F-EX) , an involuntary exercise group (I-EX) , a vascular dementia group (VD) and a sham-operation group (Sham) , with 8 rats in each group.Two-vessel occlusion (2-VO) of the arteria carotis communis was used to create a model of vascular dementia in all of the rats except those in the sham-operation group.Beginning one week after the surgery, the V-Ex rats were free to run in a running wheel.The F-EX rats were forced to run 270 m a day in an electric wheel.The I-EX rats were stimulated to imitate the gait pattern of their forelimbs running at 9 m/min three times a day for l0 minutes each time.No special training was given to the rats in the other 2 groups.Three weeks after the surgery, their learning and memory were tested using a novel object recognition test.Immediately after the test, their prefrontal cortex was sampled and the expression of SYP and PSD-95 was detected using western blotting.Results The average novel object recognition indices of the rats in the V-EX, F-EX and I-EX groups were all significantly higher than that of the VD group.Average PSD-95 expression was also significandy higher than in the VD group.Conclusion Exercise, whether voluntary, forced or induced by functional electrical stimulation can improve learning and memory in vascular dementia, at least in rats.The mechanism is possibly that the training can increase the expression of PSD-95 in the prefrontal cortex, though not SYP.