CAJ | 학술논문

目的探讨超短波对糖尿病大鼠溃疡创面局部碱性成纤维细胞生长因子(bFGF)表达的影响,及其与不同时间点糖尿病大鼠创面新生微血管数量、创面病理学改变的相关性.方法取健康成年雄性SD大鼠90只,按随机数字表法分为对照组、糖尿病组和治疗组,每组30只.糖尿病组和治疗组大鼠通过腹腔注射链脲佐菌素55 mg/kg建立糖尿病模型,90只大鼠采用去除全皮的方法制作皮肤溃疡模型,对照组和糖尿病组大鼠不进行任何干预,治疗组大鼠行超短波治疗.分别于损伤后第3、7、14和21天,取溃疡创面边缘部分组织与中心部分组织常规行苏木精-伊红(HE)染色,并进行新生微血管计数,计算半数愈合时间及痊愈时间,并在光学显微镜下观察创面病理学改变,用免疫组化法检测创面bFGF的表达情况.结果 ①对照组创面50％愈合时间和100％愈合时间分别为[(8.94 ±0.87)和(16.56±1.04)d],治疗组创面50％愈合时间和100％愈合时间[(12.78±1.26)d和(23.25±1.54)d]与糖尿病组[(13.72±1.49)和(26.08±2.23)d]比较,明显缩短,且组间差异有统计学意义(P＜0.01).②治疗组微血管计数在伤后第3、7、14和21天分别为(12.5±1.52)、(17.83±1.94)、(23.5±1.876)和(29.33±2.736)个/高倍镜视野,均多于同时间点糖尿病组,且差异均有统计学意义(P＜0.01).③治疗组的bFGF蛋白表达阳性染色指数在伤后第3、7、14和21天分别为(11.83±1.17)、(23.33±1.51)、(55.50±4.09)和(22.50±1.87),均多于同时间点糖尿病组,且差异有统计学意义(P＜0.05).结论微热量超短波治疗可增高糖尿病溃疡创面bFGF的表达量,促进创面毛细血管生成,缩短创面愈合时间,加快创面愈合.
목적 탐토초단파대당뇨병대서궤양창면국부감성성섬유세포생장인자(bFGF)표체적영향,급기여불동시간점당뇨병대서창면신생미혈관수량、창면병이학개변적상관성.방법 취건강성년웅성SD대서90지,안수궤수자표법분위대조조、당뇨병조화치료조,매조30지.당뇨병조화치료조대서통과복강주사련뇨좌균소55 mg/kg건립당뇨병모형,90지대서채용거제전피적방법제작피부궤양모형,대조조화당뇨병조대서불진행임하간예,치료조대서행초단파치료.분별우손상후제3、7、14화21천,취궤양창면변연부분조직여중심부분조직상규행소목정-이홍(HE)염색,병진행신생미혈관계수,계산반수유합시간급전유시간,병재광학현미경하관찰창면병이학개변,용면역조화법검측창면bFGF적표체정황.결과 ①대조조창면50％유합시간화100％유합시간분별위[(8.94 ±0.87)화(16.56±1.04)d],치료조창면50％유합시간화100％유합시간[(12.78±1.26)d화(23.25±1.54)d]여당뇨병조[(13.72±1.49)화(26.08±2.23)d]비교,명현축단,차조간차이유통계학의의(P＜0.01).②치료조미혈관계수재상후제3、7、14화21천분별위(12.5±1.52)、(17.83±1.94)、(23.5±1.876)화(29.33±2.736)개/고배경시야,균다우동시간점당뇨병조,차차이균유통계학의의(P＜0.01).③치료조적bFGF단백표체양성염색지수재상후제3、7、14화21천분별위(11.83±1.17)、(23.33±1.51)、(55.50±4.09)화(22.50±1.87),균다우동시간점당뇨병조,차차이유통계학의의(P＜0.05).결론 미열량초단파치료가증고당뇨병궤양창면bFGF적표체량,촉진창면모세혈관생성,축단창면유합시간,가쾌창면유합.
Objective To observe the effect of ultrashortwave irradiation on the expression of basic fibroblast growth factor (bFGF) and the growth of new capillaries and pathological changes in wounds affecting diabetics.Methods Ninety healthy, adult, male Sprague-Dawley rats were randomly divided into a control group, a diabetic group and an experimental group, each of 30.A model of diabetes was induced in each rat of the diabetic and experimental groups by intraperitoneal injection of 55 mg/kg of streptozotocin.Skin ulcers were then established by scraping.The experimental group was given ultrashortwave treatment, while the other groups were given no intervention.Three, 7, 14 and 21 days after the injury, tissues at the edge and in the central part of the ulcers were collected and given hematoxylin-eosin staining, and microvessels were counted.Any pathological changes in the ulcers were observed under an optical microscope, and the expression of bFGF was detected using immunohistochemistry.Results In the control group, the 50％ and 100％ healing times were (8.94 ± 0.87)d and (16.56 ± 1.04)d respectively.The 50％ and 100％ healing times of the experimental group were (12.78 ± 1.26) d and (23.25 ± 1.54) d-significantly shorter than those of the diabetic group.The average microvessel count of the experimental group rats increased from (12.5 ± 1.52) on day 3 to (17.83 ± 1.94) on day 7 and then to (23.5 ± 1.876) on day 14 and finally to (29.33 ± 2.736) on day 21.Those counts were significantly greater than those of the diabetic group at the same time points.The average index of bFGF expression increased from (1 1.83 ± 1.17) on day 3, to (23.33 ± 1.51) on day 7 and further to (55.50 ± 4.09) on day 21, and then decreased to (22.50 ± 1.87) on day 21, but always remaining significantly higher than in the diabetic group at the same time points.Conclusion Uhrashortwave therapy can increase the expression of bFGF, promote capillary angiogenesis and quicken the healing of diabetics' wounds.