特产研究
特產研究
특산연구
Special Wild Economic Animal and Plant Research
2015年
3期
34-37
,共4页
韩威%王怀生%仇一晶%郑美娇%杨映霞%孙玉滨%李玉明
韓威%王懷生%仇一晶%鄭美嬌%楊映霞%孫玉濱%李玉明
한위%왕부생%구일정%정미교%양영하%손옥빈%리옥명
山萸肉桂片%提取工艺%正交实验%高效液相色谱法
山萸肉桂片%提取工藝%正交實驗%高效液相色譜法
산유육계편%제취공예%정교실험%고효액상색보법
Cornus officinalis cinnamon tablets%extraction process%orthogonal experiment%HPLC
研究山萸肉桂片的提取工艺。用正交设计实验分别对处方中发挥主要药理作用的桑叶中的芦丁以及丹参中的丹参酮ⅡA进行了提取分离,并通过高效液相色谱法进行含量分析,分别确定了桑叶与丹参最佳提取工艺,即桑叶用10倍量30%乙醇提取2次,每次1.5h;丹参用10倍量75%乙醇提取2次,每次1.5h。实验证明该工艺合理,方法可行。
研究山萸肉桂片的提取工藝。用正交設計實驗分彆對處方中髮揮主要藥理作用的桑葉中的蘆丁以及丹參中的丹參酮ⅡA進行瞭提取分離,併通過高效液相色譜法進行含量分析,分彆確定瞭桑葉與丹參最佳提取工藝,即桑葉用10倍量30%乙醇提取2次,每次1.5h;丹參用10倍量75%乙醇提取2次,每次1.5h。實驗證明該工藝閤理,方法可行。
연구산유육계편적제취공예。용정교설계실험분별대처방중발휘주요약리작용적상협중적호정이급단삼중적단삼동ⅡA진행료제취분리,병통과고효액상색보법진행함량분석,분별학정료상협여단삼최가제취공예,즉상협용10배량30%을순제취2차,매차1.5h;단삼용10배량75%을순제취2차,매차1.5h。실험증명해공예합리,방법가행。
To study the extraction process of Cornus officinalis Cinnamon tablets .Mulberry Leaf and Salvia miltiorrhiza played a major role in the pharmacological prescription ,Rutin from Mulberry Leaf and tanshinoneⅡA from Salvia miltiorrhiza were extracted by using orthogonal experi-mental design and their comtent was analyzed by HPLC .Result:The better process parameters for Salvia miltiorrhiza and Mulberry Leaf were de-termined ,that was extracted the Salvia miltiorrhiza with ten times volume 75% ethanol for two times and each time last for 1.5 hours ,and ex-tracted the Mulberry Leaf with ten times volume 30% ethanol for two times and each time last for 1.5 hours .Experiments show that the process is reasonable ,feasible .