中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
10期
2450-2452
,共3页
马先仕%易丽华%王雄彪%肖绪鹏
馬先仕%易麗華%王雄彪%肖緒鵬
마선사%역려화%왕웅표%초서붕
长链非编码RNA SPRY4-IT1%结肠癌%增殖%侵袭
長鏈非編碼RNA SPRY4-IT1%結腸癌%增殖%侵襲
장련비편마RNA SPRY4-IT1%결장암%증식%침습
Long non-coding RNASPRY4-IT1%Colonic cancer%Proliferation%Invasion
目的 观察长链非编码RNA SPRY4-IT1(lncRNA SPRY4-IT1)对结肠癌细胞株SW48恶性生物学行为的影响.方法 合成针对lncRNA SPRY4-IT1的特异性小干扰RNA(si-SPRY4-IT1),转染SW48细胞,利用实时定量反转录聚合酶链反应(RT-qPCR)技术检测SW48细胞中SPRY4-IT1表达量,验证siRNA效果;细胞计数试剂盒(CCK-8)增殖实验检测低表达lncRNA SPRY4-IT1对SW48细胞增殖能力的影响;膜联蛋白V-异硫氰酸荧光素(Annexin V-FITC)/碘化丙锭(PI)染色流式检测法检测对凋亡的影响;Transwell侵袭实验观察对细胞侵袭能力的影响.结果 RT-qPCR证实与对照组比较(0.923±0.025),si-SPRY4-IT1可使lncRNA SPRY4-IT1表达量(0.312±0.034)明显降低;CCK-8增殖实验中,lncRNA SPRY4-IT1低表达可抑制SW48细胞的增殖能力(P＜0.05);凋亡实验中,低表达SPRY4-IT1细胞的凋亡率[(15.140±0.680)％]较对照组细胞凋亡率[(4.223±0.724)％]明显增高(P＜0.05);侵袭实验中低表达SPRY4-IT1细胞穿膜数目为(29.6±4.2)个,较对照组(92.3±2.5)个明显减少(P＜0.05).结论 低表达lncRNA SPRY4-IT1可抑制结肠癌细胞SW48增殖和侵袭能力,促进其凋亡.
目的 觀察長鏈非編碼RNA SPRY4-IT1(lncRNA SPRY4-IT1)對結腸癌細胞株SW48噁性生物學行為的影響.方法 閤成針對lncRNA SPRY4-IT1的特異性小榦擾RNA(si-SPRY4-IT1),轉染SW48細胞,利用實時定量反轉錄聚閤酶鏈反應(RT-qPCR)技術檢測SW48細胞中SPRY4-IT1錶達量,驗證siRNA效果;細胞計數試劑盒(CCK-8)增殖實驗檢測低錶達lncRNA SPRY4-IT1對SW48細胞增殖能力的影響;膜聯蛋白V-異硫氰痠熒光素(Annexin V-FITC)/碘化丙錠(PI)染色流式檢測法檢測對凋亡的影響;Transwell侵襲實驗觀察對細胞侵襲能力的影響.結果 RT-qPCR證實與對照組比較(0.923±0.025),si-SPRY4-IT1可使lncRNA SPRY4-IT1錶達量(0.312±0.034)明顯降低;CCK-8增殖實驗中,lncRNA SPRY4-IT1低錶達可抑製SW48細胞的增殖能力(P＜0.05);凋亡實驗中,低錶達SPRY4-IT1細胞的凋亡率[(15.140±0.680)％]較對照組細胞凋亡率[(4.223±0.724)％]明顯增高(P＜0.05);侵襲實驗中低錶達SPRY4-IT1細胞穿膜數目為(29.6±4.2)箇,較對照組(92.3±2.5)箇明顯減少(P＜0.05).結論 低錶達lncRNA SPRY4-IT1可抑製結腸癌細胞SW48增殖和侵襲能力,促進其凋亡.
목적 관찰장련비편마RNA SPRY4-IT1(lncRNA SPRY4-IT1)대결장암세포주SW48악성생물학행위적영향.방법 합성침대lncRNA SPRY4-IT1적특이성소간우RNA(si-SPRY4-IT1),전염SW48세포,이용실시정량반전록취합매련반응(RT-qPCR)기술검측SW48세포중SPRY4-IT1표체량,험증siRNA효과;세포계수시제합(CCK-8)증식실험검측저표체lncRNA SPRY4-IT1대SW48세포증식능력적영향;막련단백V-이류청산형광소(Annexin V-FITC)/전화병정(PI)염색류식검측법검측대조망적영향;Transwell침습실험관찰대세포침습능력적영향.결과 RT-qPCR증실여대조조비교(0.923±0.025),si-SPRY4-IT1가사lncRNA SPRY4-IT1표체량(0.312±0.034)명현강저;CCK-8증식실험중,lncRNA SPRY4-IT1저표체가억제SW48세포적증식능력(P＜0.05);조망실험중,저표체SPRY4-IT1세포적조망솔[(15.140±0.680)％]교대조조세포조망솔[(4.223±0.724)％]명현증고(P＜0.05);침습실험중저표체SPRY4-IT1세포천막수목위(29.6±4.2)개,교대조조(92.3±2.5)개명현감소(P＜0.05).결론 저표체lncRNA SPRY4-IT1가억제결장암세포SW48증식화침습능력,촉진기조망.
Objective To investigate the effectof long non-coding RNASPRY4-IT1 (IncRNSPRY4-IT1) on malignanbiological behaviorof colonicancecell line SW48.MethodSynthesized small interfering RN(siRNA) targeting lncRNSPRY4-IT1 (si-SPRY4-IT1) watransfected into SW48 cells, then the expression of lncRNSPRY4-IT1 wadetected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR).The cell viability wadetected by cell counting kit-8 (CCK-8), the cell apoptosiwadetected by Annexin V-fluoresceine isothiocyanate (FITC)/propidium iodide (PI) and the cell invasion waexamined by Transwell assay.ResultCompared to the control,the expression of lncRNSPRY4-IT1 wasignificantly decreased in the experimental group (0.312 ± 0.034 vs.0.923 ± 0.025).The proliferation assay showed the proliferation ability of the experimental group wasignificantly decreased acompared with the control group (P ＜ 0.05).The si-SPRY4-IT1 promoted cell apoptosiand inhibited migration (P ＜ 0.05).Conclusion Down-regulation of SPRY4-IT1 could inhibithe proliferation and invasion capability, and promote the apoptosiof human colonicancecell SW48.
