CAJ | 학술논문

目的探讨利用血管内皮细胞(VEC)实现海藻酸人工肝组织支架(AS)血管化的可能.方法构建VEC-AS复合物,通过显微镜及噻唑蓝(MTT)法检测两者相容性.采用70％肝切除模型,实验组(A组)给予VEC-AS复合物,对照组(B组)给予AS.术后第14、28天处死大鼠,大体及切片检查观察两组大鼠移植物表面及内部血管形成.结果显微镜下观察VEC能够在AS上良好的黏附、增殖;MTT法检测VEC在AS上1～3 d进入生长潜伏期,第4～7天进入快速增长期,第16～19天开始进入平台期.14 d后移植物表面观察到新生血管,切片检查发现新生血管从周围肝组织向移植物长入,血管内见红细胞;28 d移植物周围血管增粗增多,切片检查发现移植物内血管明显增粗,血管内红细胞增多,中心亦可见大量新生血管.而B组内从术后第7 ～ 28天均未见任何血管长人.结论 VEC和AS具有良好生物相容性,利用VEC可以在体内初步实现AS血管化.
목적 탐토이용혈관내피세포(VEC)실현해조산인공간조직지가(AS)혈관화적가능.방법 구건VEC-AS복합물,통과현미경급새서람(MTT)법검측량자상용성.채용70％간절제모형,실험조(A조)급여VEC-AS복합물,대조조(B조)급여AS.술후제14、28천처사대서,대체급절편검사관찰량조대서이식물표면급내부혈관형성.결과 현미경하관찰VEC능구재AS상량호적점부、증식;MTT법검측VEC재AS상1～3 d진입생장잠복기,제4～7천진입쾌속증장기,제16～19천개시진입평태기.14 d후이식물표면관찰도신생혈관,절편검사발현신생혈관종주위간조직향이식물장입,혈관내견홍세포;28 d이식물주위혈관증조증다,절편검사발현이식물내혈관명현증조,혈관내홍세포증다,중심역가견대량신생혈관.이B조내종술후제7 ～ 28천균미견임하혈관장인.결론 VEC화AS구유량호생물상용성,이용VEC가이재체내초보실현AS혈관화.
Objective To study the vascularization of alginate scaffold by vasculaendothelial cellfohepatitissue engineering.MethodVasculaendothelial cell(VEC) and alginate scaffold (AS) were used to form the VEC-AS-complex.The compatibility of VEand Awaobsenred by microscopy and the tesof methyl thiazol tetrazolium (MTT).In vivo teswacarried in two groupof 70％ liveresection rats, group with VEC-AS-complex transplanted in the livewound and group with Aonly.Vascularization of the complex waobserved on the 14th and 28th aftethe transplantation.ResultVEwafound to be able to attach, grow and proliferate well in the Aby microscopy and MTT.In group A, neovascularization could be observed around and in the complex on 14th day aftetransplantation, the vasculabecame largeand largewith the dayon 28th day aftetransplatation.While in group B, none vasculacould be observed.Conclusion VEand Aexhibited well compatibility.VEcould be used athe way to make the vascularization of Afohepatitissue engineering.