CAJ | 학술논문

目的观察经尾静脉、腹膜腔或肝内注射3种不同途径移植骨髓间充质干细胞(B MSCs)对大鼠肝纤维化进程的影响.方法建立四氯化碳(CCl4)诱导的大鼠肝纤维化模型,第6周时BMSCs分别经尾静脉、腹膜腔或肝内注射途径回输.造模12周结束时评价大鼠存活率、腹水发生、炎症及纤维化.结果造模12周结束时4组(n=16)生存率差异无统计学意义(x2=0.82,P ＞0.05),但腹水发生率差异有统计学意义(x2=9.019,P＜0.05).仅经尾静脉移植组炎症活动度、纤维化以及α-平滑肌肌动蛋白(α-SMA)表达均低于对照组(P＜0.01).结论经外周静脉移植BMSCs能减轻大鼠肝脏炎症和α-SMA表达、减轻肝纤维化.
목적 관찰경미정맥、복막강혹간내주사3충불동도경이식골수간충질간세포(B MSCs)대대서간섬유화진정적영향.방법 건립사록화탄(CCl4)유도적대서간섬유화모형,제6주시BMSCs분별경미정맥、복막강혹간내주사도경회수.조모12주결속시평개대서존활솔、복수발생、염증급섬유화.결과 조모12주결속시4조(n=16)생존솔차이무통계학의의(x2=0.82,P ＞0.05),단복수발생솔차이유통계학의의(x2=9.019,P＜0.05).부경미정맥이식조염증활동도、섬유화이급α-평활기기동단백(α-SMA)표체균저우대조조(P＜0.01).결론 경외주정맥이식BMSCs능감경대서간장염증화α-SMA표체、감경간섬유화.
Objective To investigate the effecof the bone narrow mesenchymal stem cell(BMSCs) transplantation vidifferenadministration routein the treatmenof carbon tetrachloride (CCl4)-induced livefibrosiin rats.MethodThe hepatifibrosimodelwere induced by CCl4 in rats.The BMSCof the forth generation (5 × 1010/L) were injected into the livedirectly, viintraperitoneal injection ovithe caudalivenon the sixth week of the hepatifibrosimodeling in rats.Six weekafteBMSCtransplantation, the pathological changeof the livewere evaluated by Scheueinflammation activity score and Schmid M fibrosigrading score respectively.Also, the activation stage of HSin the livewadetected by immunohistochemistry and Western blotting.ResultSix weekafteBMSCtransplantation, there were obviously differencein asciteincidence among fougroup(x2 =9.019, P ＜ 0.05).Acompared with the control group, the inflammation activity grade, fibrosigrade and α-smooth muscle actin (α-SMA) expression in the group with BMSCtranplantation vithe caudalivenwere significantly decreased (P ＜ 0.01).Conclusion BMSCtransplantation vithe caudalivencould alleviate inflammation reaction,lessen the activation stage of HSin the liveand improve fibrosistage in transplanted ratwith livecirrhosiinduced by CCl4.