中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
10期
2448-2449
,共2页
许鹤洋%曾育杰%罗兴喜%来伟%蓝球生%褚忠华
許鶴洋%曾育傑%囉興喜%來偉%藍毬生%褚忠華
허학양%증육걸%라흥희%래위%람구생%저충화
肝再生磷酸酶3%结肠癌%有氧糖酵解
肝再生燐痠酶3%結腸癌%有氧糖酵解
간재생린산매3%결장암%유양당효해
Liver regeneration phosphatase 3%Colon cancer%Aerobic glycolysis
目的 观察肝再生磷酸酶3(PRL-3)在结肠癌细胞中是否参与调控细胞代谢并促进有氧糖酵解作用.方法 将PRL-3转染入结肠癌LoVo细胞中,检测细胞(1×106个细胞)乳酸代谢的水平;通过实时定量反转录聚合酶链反应(RT-qPCR)检测对应LoVo细胞(1×106个细胞)糖酵解相关酶的表达水平,并通过Western blot进行验证.结果 转染PRL-3的LoVo细胞(LoVo-P)乳酸表达明显高于转染空白质粒的LoVo细胞(LoVo-C)及LoVo细胞([11.9 ±2.7) mol/L比(4.2±1.6) mol/L比(3.7±1.2)mol/L,P＜0.05].而RT-qPCR结果显示LoVo-P细胞的糖酵解关键酶磷酸丙酮酸激酶M2(PKM2)、葡萄糖转运蛋白1(Glut1)、丙酮酸脱氢酶(PDH)表达较LoVo-C升高,并且Western blot验证显示PKM2、Glut1、PDH蛋白表达上升.结论 PRL-3能够调控LoVo细胞的代谢水平,通过促进LoVo细胞有氧糖酵解关键酶表达上调,使肿瘤细胞更好地适应环境.
目的 觀察肝再生燐痠酶3(PRL-3)在結腸癌細胞中是否參與調控細胞代謝併促進有氧糖酵解作用.方法 將PRL-3轉染入結腸癌LoVo細胞中,檢測細胞(1×106箇細胞)乳痠代謝的水平;通過實時定量反轉錄聚閤酶鏈反應(RT-qPCR)檢測對應LoVo細胞(1×106箇細胞)糖酵解相關酶的錶達水平,併通過Western blot進行驗證.結果 轉染PRL-3的LoVo細胞(LoVo-P)乳痠錶達明顯高于轉染空白質粒的LoVo細胞(LoVo-C)及LoVo細胞([11.9 ±2.7) mol/L比(4.2±1.6) mol/L比(3.7±1.2)mol/L,P＜0.05].而RT-qPCR結果顯示LoVo-P細胞的糖酵解關鍵酶燐痠丙酮痠激酶M2(PKM2)、葡萄糖轉運蛋白1(Glut1)、丙酮痠脫氫酶(PDH)錶達較LoVo-C升高,併且Western blot驗證顯示PKM2、Glut1、PDH蛋白錶達上升.結論 PRL-3能夠調控LoVo細胞的代謝水平,通過促進LoVo細胞有氧糖酵解關鍵酶錶達上調,使腫瘤細胞更好地適應環境.
목적 관찰간재생린산매3(PRL-3)재결장암세포중시부삼여조공세포대사병촉진유양당효해작용.방법 장PRL-3전염입결장암LoVo세포중,검측세포(1×106개세포)유산대사적수평;통과실시정량반전록취합매련반응(RT-qPCR)검측대응LoVo세포(1×106개세포)당효해상관매적표체수평,병통과Western blot진행험증.결과 전염PRL-3적LoVo세포(LoVo-P)유산표체명현고우전염공백질립적LoVo세포(LoVo-C)급LoVo세포([11.9 ±2.7) mol/L비(4.2±1.6) mol/L비(3.7±1.2)mol/L,P＜0.05].이RT-qPCR결과현시LoVo-P세포적당효해관건매린산병동산격매M2(PKM2)、포도당전운단백1(Glut1)、병동산탈경매(PDH)표체교LoVo-C승고,병차Western blot험증현시PKM2、Glut1、PDH단백표체상승.결론 PRL-3능구조공LoVo세포적대사수평,통과촉진LoVo세포유양당효해관건매표체상조,사종류세포경호지괄응배경.
Objective To investigate whetheliveregeneration phosphatase 3 (PRL-3) participatein the regulation of colon cancecell metabolism, and enhancethe aerobiglycolysis.MethodPRL-3 wastable transfected into LoVo cells, cell (1 × 106) medium wacollected to detecthe level of lactate acid.Besides, real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blowaused to detecthe expression of related enzymeof glycolysiin LoVo cell(1 × 106).ResultLoVo cellwhich were transfected with PRL-3 (LoVo-P) expressed more lactate than LoVo celltransfected with Vector-Control (LoVo-C) and LoVo cell[(11.9 ± 2.7) mol/L vs.(4.2 ± 1.6) mol/L vs.(3.7 ± 1.2) mol/L, P ＜ 0.05].Besides, RT-qPCrevealed thaphosphorylatepyruvate kinase M2 (PKM2), glucose transporte1 (Glut1), pyruvate dehydrogenase (PDH) increased in LoVo-P, which are the key enzyme in glycolysis.Wrstern bloalso tested the same resultin the protein level.Conclusion PRL-3 regulateLoVo cellmetabolism and enhance the aerobiglycolysis, in thiwav LoVo cellcan betteadapthe tumomicroenvironment.
