CAJ | 학술논문

依照GB／T 27859—2011《沉积物－水系统中摇蚊毒性试验加标于沉积物法》，以死亡率、羽化率、生长速率、羽化用时、雌雄比、卵筏产量及孵化率为观测指标，研究了沉积物中多杀霉素对摇蚊（ Chironomus tepperi）完整生命周期的慢性毒性效应。结果表明，幼虫的生长速率和雌性幼虫羽化用时与多杀霉素浓度呈正相关，幼虫的羽化率及存活率与多杀霉素浓度呈负相关，而雄性幼虫羽化用时以及成虫雌雄比与多杀霉素浓度不相关；28 d试验后多杀霉素对摇蚊幼虫的半致死浓度w（ LC50）为1626?96μg·kg－1，对其羽化率的半数效应浓度w（ EC50）为1633?53μg·kg－1。当w（多杀霉素）低于10μg·kg－1时，与对照相比受试生物的羽化用时和生长速率反而会缩短和增加；沉积物中多杀霉素对摇蚊卵筏产量无显著影响，而对各卵筏中摇蚊卵数量及孵化出的1龄摇蚊幼虫数量存在显著影响（ P＜0?05）。
의조GB／T 27859—2011《침적물－수계통중요문독성시험가표우침적물법》，이사망솔、우화솔、생장속솔、우화용시、자웅비、란벌산량급부화솔위관측지표，연구료침적물중다살매소대요문（ Chironomus tepperi）완정생명주기적만성독성효응。결과표명，유충적생장속솔화자성유충우화용시여다살매소농도정정상관，유충적우화솔급존활솔여다살매소농도정부상관，이웅성유충우화용시이급성충자웅비여다살매소농도불상관；28 d시험후다살매소대요문유충적반치사농도w（ LC50）위1626?96μg·kg－1，대기우화솔적반수효응농도w（ EC50）위1633?53μg·kg－1。당w（다살매소）저우10μg·kg－1시，여대조상비수시생물적우화용시화생장속솔반이회축단화증가；침적물중다살매소대요문란벌산량무현저영향，이대각란벌중요문란수량급부화출적1령요문유충수량존재현저영향（ P＜0?05）。
By following the guideline ( GB/T 27859—2011) of“Spiking Sediment in Toxicity Test on Chironomus tepperi in Sediment?Water System”, tests were carried out using mortality, emergence rate, growth rate, time for emerging, sex ratio, and yield of eggrafts and hatchability as observation targets to study chronic toxic effect of spinosad residue in sedi?ment on larvae and eggrafts of Chironomus tepperi. It was found that growth rate of larvae and average time for female adults to emerge were positively related to spinosad concentration spiked, while survival rate and emergence rate were negatively related and average time for male adults to emerge and sex ratio of adults were not related. The tests indicate that the 28 d LC50 of spinosad for Chironomus tepperi was 1 626?96μg·kg-1 , and the EC50 ( half maximal effective concentration) on emergence was 1 633?53 μg·kg-1 . When the concentrations of spiked spinosad were lower than 10μg·kg-1 , advanced emergence and higher growth rate of Chironomus tepperi could be observed as compared with the control. Eggraft yield of Chironomus tepperi was not significantly affected by spinosad in the sediment, while the number of eggs in each eggraft and the number of first?instar larvaes hatched was significantly affected.