中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
10期
2586-2589
,共4页
前列腺增生%前列腺炎%肿瘤坏死因子-α%白细胞介素-6%CD3
前列腺增生%前列腺炎%腫瘤壞死因子-α%白細胞介素-6%CD3
전렬선증생%전렬선염%종류배사인자-α%백세포개소-6%CD3
Benign prostatic hyperplasia%Prostatitis%Tumor necrosis factor-alpha%Interleukin-6%CD3
目的 探讨患者前列腺组织及血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)以及CD3的表达改变及临床意义.方法 60例因尿潴留或下尿路症状(LUTS)行经尿道前列腺切除术(TURP)的前列腺增生(BPH)患者,根据病理诊断分为单纯前列腺增生组15例和前列腺增生合并炎症组共45例,其中轻度炎症12例,中度炎症25例,重度炎症8例.所有患者中按有无尿潴留病史,分为尿潴留组20例和LUTS 40例,国际前列腺症状评分(IPSS)＞ 25分,为高IPSS组32例,IPSS在19 ～ 25之间为低IPSS组28例.TURP术中取前列腺组织,苏木素-伊红(HE)染色,采用免疫组织化学法及Western blot方法测定组织中TNF-α、IL-6以及CD3的表达及含量.结果 免疫组织化学染色结果显示BPH合并炎症组TNF-α、IL-6、CD3表达吸光度(A)值(0.0524±0.0400、0.033 4±0.024 0、0.036 5±0.0090)高于BPH组(0.0323 ±0.6000、0.2040±0.0160、0.022 3±0.003 0),Westem blot方法检测结果显示BPH合并炎症组TNF-α、IL-6、CD3表达相对量(0.24±0.04、0.07 ±0.04、0.33 ±0.09)高于BPH组(0.19±0.06、0.04±0.02、0.27±0.06).尿潴留组各促炎性细胞因子含量明显高于LUTS组,高IPSS评分组各促炎性细胞因子含量高于低IPSS评分组.前列腺增生症合并不同程度炎症中,以重度炎症升高最为显著.结论 炎症刺激下促炎因子表达明显增多,使前列腺上皮及间质细胞增殖,导致前列腺体积增大,促进疾病进展.
目的 探討患者前列腺組織及血清中腫瘤壞死因子-α(TNF-α)、白細胞介素-6(IL-6)以及CD3的錶達改變及臨床意義.方法 60例因尿潴留或下尿路癥狀(LUTS)行經尿道前列腺切除術(TURP)的前列腺增生(BPH)患者,根據病理診斷分為單純前列腺增生組15例和前列腺增生閤併炎癥組共45例,其中輕度炎癥12例,中度炎癥25例,重度炎癥8例.所有患者中按有無尿潴留病史,分為尿潴留組20例和LUTS 40例,國際前列腺癥狀評分(IPSS)＞ 25分,為高IPSS組32例,IPSS在19 ～ 25之間為低IPSS組28例.TURP術中取前列腺組織,囌木素-伊紅(HE)染色,採用免疫組織化學法及Western blot方法測定組織中TNF-α、IL-6以及CD3的錶達及含量.結果 免疫組織化學染色結果顯示BPH閤併炎癥組TNF-α、IL-6、CD3錶達吸光度(A)值(0.0524±0.0400、0.033 4±0.024 0、0.036 5±0.0090)高于BPH組(0.0323 ±0.6000、0.2040±0.0160、0.022 3±0.003 0),Westem blot方法檢測結果顯示BPH閤併炎癥組TNF-α、IL-6、CD3錶達相對量(0.24±0.04、0.07 ±0.04、0.33 ±0.09)高于BPH組(0.19±0.06、0.04±0.02、0.27±0.06).尿潴留組各促炎性細胞因子含量明顯高于LUTS組,高IPSS評分組各促炎性細胞因子含量高于低IPSS評分組.前列腺增生癥閤併不同程度炎癥中,以重度炎癥升高最為顯著.結論 炎癥刺激下促炎因子錶達明顯增多,使前列腺上皮及間質細胞增殖,導緻前列腺體積增大,促進疾病進展.
목적 탐토환자전렬선조직급혈청중종류배사인자-α(TNF-α)、백세포개소-6(IL-6)이급CD3적표체개변급림상의의.방법 60례인뇨저류혹하뇨로증상(LUTS)행경뇨도전렬선절제술(TURP)적전렬선증생(BPH)환자,근거병리진단분위단순전렬선증생조15례화전렬선증생합병염증조공45례,기중경도염증12례,중도염증25례,중도염증8례.소유환자중안유무뇨저류병사,분위뇨저류조20례화LUTS 40례,국제전렬선증상평분(IPSS)＞ 25분,위고IPSS조32례,IPSS재19 ～ 25지간위저IPSS조28례.TURP술중취전렬선조직,소목소-이홍(HE)염색,채용면역조직화학법급Western blot방법측정조직중TNF-α、IL-6이급CD3적표체급함량.결과 면역조직화학염색결과현시BPH합병염증조TNF-α、IL-6、CD3표체흡광도(A)치(0.0524±0.0400、0.033 4±0.024 0、0.036 5±0.0090)고우BPH조(0.0323 ±0.6000、0.2040±0.0160、0.022 3±0.003 0),Westem blot방법검측결과현시BPH합병염증조TNF-α、IL-6、CD3표체상대량(0.24±0.04、0.07 ±0.04、0.33 ±0.09)고우BPH조(0.19±0.06、0.04±0.02、0.27±0.06).뇨저류조각촉염성세포인자함량명현고우LUTS조,고IPSS평분조각촉염성세포인자함량고우저IPSS평분조.전렬선증생증합병불동정도염증중,이중도염증승고최위현저.결론 염증자격하촉염인자표체명현증다,사전렬선상피급간질세포증식,도치전렬선체적증대,촉진질병진전.
Objective To study the expression and clinical significance of proinflammatory cytokin[tumonecrosifactoalph(TNF-α t), interleukin-6 (IL-6) and CD3] in prostate tissue and serum in 60 casewhich received transurethral resection of the prostate (TURP) due to urinary retention olow urinary tracsymptom(LUTS).MethodSixty patientwith benign prostatihyperplasi(BPH) who received TURP fourinary retention oLUTwere divided into BPH group (15 cases) and BPH with prostatitigroup (45 cases).The general information wacollected.Prostate tissue waobtained from the transition zone of the urethrduring TURP, then immediately stored in the-80 ℃ condition.TNF-α,IL-6 and CD3 were detected by Western blotting in the prostate tissue.The expression of TNF-α, IL-6 and CD3 wadetected by immunohistochemistry to insure site of these in prostate tissue.Between the BPH and BPH with prostatitigroups, urinary retention and LUTgroups, ohigh International Prostate Symptom Score(IPSS) and low IPSgroups, the expression of TNF-α, IL-6, and CD3 were compared in the prostatitissue.ResultThe expression of TNF-α, IL-6, and CD3 in BPH with prostatitigroup wasignificantly highethan thain BPH group.The levelof proinflammatory cytokinin urinary retention group wasignificantly highethan thain LUTgroup.The expression of TNF-o, IL-6, and CD3 in high IPSgroup (IPS＞ 25) wahighethan in low IPSgroup (IPS19-25).The expression of TNF-α, IL-6, and CD3 among mild, moderate, severe inflammation in BPH group also showed significandifferences.The expression of TNF-α, IL-6, and CD3 in severe inflammation in BPH group wahighest.Conclusion TNF-o, IL-6, and CD3 levelincreased in BPH with prostatitiin local prostate tissue, and the serum TNF-α and IL-6 also increased.TNF-α and IL-6 levelalso increased in the urinary retention and high IPSscore groupin local prostate tissue and serum.Proinflammatory factors,TNF-α and IL-6, and CD3 were significantly increased in inflammatory stimuli reaction, may participate in the pathogenesiof chroniinflammation in prostate hyperplasiand wainduced by proinflammatory factors, which could be used to evaluate the moleculabiology in LUTS.
