CAJ | 학술논문

目的：探讨微管相关蛋白LC3-II与川崎病(KD)炎症反应的关系。方法选入39例急性期静脉注射丙种球蛋白前的KD患儿，根据心脏彩超结果，20例KD伴冠状动脉损伤(CAL)组，19例不伴冠状动脉损伤(NCAL)组；同时以12例正常儿童作为对照组。采集血清标本，加入人冠状动脉内皮细胞(HCAEC)体外培养体系干预12 h。采用Western-blotting、Q-PCR分别测定HCAEC中LC3-II蛋白及mRNA的表达。结果干预12 h后，CAL组、NCAL组的LC3-II蛋白及mRNA表达均较对照组明显升高，且CAL组更高于NCAL组，差异均有统计学意义(P<0.05)。结论自噬参与了急性期KD的炎症反应，可能与KD患儿冠状动脉内皮细胞损害有关。
목적：탐토미관상관단백LC3-II여천기병(KD)염증반응적관계。방법선입39례급성기정맥주사병충구단백전적KD환인，근거심장채초결과，20례KD반관상동맥손상(CAL)조，19례불반관상동맥손상(NCAL)조；동시이12례정상인동작위대조조。채집혈청표본，가입인관상동맥내피세포(HCAEC)체외배양체계간예12 h。채용Western-blotting、Q-PCR분별측정HCAEC중LC3-II단백급mRNA적표체。결과간예12 h후，CAL조、NCAL조적LC3-II단백급mRNA표체균교대조조명현승고，차CAL조경고우NCAL조，차이균유통계학의의(P<0.05)。결론자서삼여료급성기KD적염증반응，가능여KD환인관상동맥내피세포손해유관。
ObjectiveTo investigate the relationship between microtubule-associated protein l light chain 3B (LC3-II) and the inlfammatory response of Kawasaki disease (KD).MethodsThirty-nine cases of acute KD before intravenous admin-istration of immunoglobulin were enrolled. According to the results of echocardiography, the 39 cases were furtherly divided into KD with coronary artery lesion (CAL, 20 cases) group and KD with non-CAL (NCAL, 19 cases) group. At the same time, 12 healthy children were selected as controls. Serum samples were collected and cultured in vitro by human coronary artery endothe-lial cells (HCAEC) for 12 h. The LC3-II protein and mRNA expression of HCAEC were detected by Western-blotting and Q-PCR respectively.ResultsThe LC3-II protein and mRNA expression in CAL group and NCAL group were signiifcantly higher than those in control group, the LC3-II protein and mRNA expression in CAL group was higher than those in NCAL group, and both differences were statistically signiifcant (P<0.05).ConclusionsAutophagy may be involved in the inlfammatory response of KD in acute phase, which may be related to endothelial cell lesions of coronary artery in children with KD.