CAJ | 학술논문

目的：探讨不同缺氧时间对3日龄SD大鼠脑白质损伤程度的影响。方法新生大鼠随机分为正常组（n=24）、模型组（n=45），其中模型组再根据缺氧时间50、70、90 min分为3个亚组（n=15）；模型组采用单侧结扎颈总动脉的方法制作脑白质损伤动物模型。经过不同时间缺氧后，统计造模死亡率，苏木精-伊红（HE）染色观察脑病理形态改变，免疫荧光染色检测脑白质特异性标志物髓鞘碱性蛋白（MBP）变化，爬坡试验检测运动功能。结果随着缺氧时间延长造模死亡率明显升高，90 min组死亡率高达60%；HE染色结果显示，50 min组白质和海马基本无损伤，70 min组手术侧选择性白质损伤，而90 min组白质、海马、皮质均发生大范围梗死。MBP半定量白质损伤评分显示，70 min组（3.89±0.47）和90 min组（4.72±0.57）明显高于正常组（0.06±0.24），差异有统计学意义（P<0.05）。爬坡实验，模型组各亚组大鼠均出现不同程度的患侧运动功能障碍，以90 min组较为严重。结论采用单侧结扎颈总动脉法成功制作脑白质损伤模型；不同缺氧时间对模型病变范围以及损伤程度具有重要影响。
목적：탐토불동결양시간대3일령SD대서뇌백질손상정도적영향。방법신생대서수궤분위정상조（n=24）、모형조（n=45），기중모형조재근거결양시간50、70、90 min분위3개아조（n=15）；모형조채용단측결찰경총동맥적방법제작뇌백질손상동물모형。경과불동시간결양후，통계조모사망솔，소목정-이홍（HE）염색관찰뇌병리형태개변，면역형광염색검측뇌백질특이성표지물수초감성단백（MBP）변화，파파시험검측운동공능。결과수착결양시간연장조모사망솔명현승고，90 min조사망솔고체60%；HE염색결과현시，50 min조백질화해마기본무손상，70 min조수술측선택성백질손상，이90 min조백질、해마、피질균발생대범위경사。MBP반정량백질손상평분현시，70 min조（3.89±0.47）화90 min조（4.72±0.57）명현고우정상조（0.06±0.24），차이유통계학의의（P<0.05）。파파실험，모형조각아조대서균출현불동정도적환측운동공능장애，이90 min조교위엄중。결론채용단측결찰경총동맥법성공제작뇌백질손상모형；불동결양시간대모형병변범위이급손상정도구유중요영향。
ObjectiveTo explore the effect of different hypoxic duration on the brain white matter injury.Methods ewborn rats were randomly divided into two groups, normal group (n=24) and model group (n=45). The model group was di-vided into 3 subgroups (n=15) according to the time of hypoxia (50 min, 70 min, and 90 min). The animal model of white matter injury was established by unilateral carotid artery ligation in model group. After different duration of hypoxia, the mortality rate was recorded, the morphological changes of brain pathology was observed by hematoxylin eosin (HE) staining, myelin basic pro-tein (MPB) of white matter was detected by immunolfuorescence staining and motor function was evaluated by climbing slope test.ResultsThe mortality rates signiifcantly increased with prolonged hypoxia. The mortality rate was as high as 60% in 90 min subgroup. The HE staining showed that there were no obvious injury in 50 min subgroup, selective white matter injury on the operative side appeared in 70 min subgroup, and a wide range of infarction of white matter, hippocampus, and cortex appeared in 90 min subgroup. MBP semi-quantitative scores of white matter injury were higher in 70 min subgroup (3.89 ± 0.47) and 90 min subgroup (4.72 ± 0.57) than that in the normal group (0.06 ± 0.24), the difference was statistically signiifcant (P <0.05). In climb-ing slope test, the subgroups had different degrees of motor dysfunction on affected side with 90 min subgroup being the most serious.ConclusionsWhite matter injury model could be established by unilateral carotid artery ligation, and different hypoxic duration signiifcantly affects the range and degree of injury.