CAJ | 학술논문

目的描述临床分离的热带假丝酵母菌培养不同时间的天冬氨酰蛋白酶活性(蛋白酶活性)、磷脂酶活性和溶血活性,并分析不同感染部位分离菌的蛋白酶活性和溶血活性差异.方法将分离自不同感染部位的热带假丝酵母菌鉴定后分别接种在牛血清蛋白培养基、卵黄琼脂培养基和羊血琼脂培养基上,培养不同时间(24、48和72 h)后检测蛋白酶活性、磷脂酶活性和溶血活性.结果热带假丝酵母菌培养24、48和72 h时均具有蛋白酶活性和溶血活性,但未表现出磷脂酶活性;热带假丝酵母菌在48和72 h时的蛋白酶活性高于其在24 h时的活性,在72 h范围内,溶血活性随培养时间延长持续增长;分离自不同感染部位的热带假丝酵母菌的蛋白酶活性(P=0.368)和溶血活性(P=0.985)差异无统计学意义.结论我国热带假丝酵母菌临床分离株培养不同时间具有蛋白酶活性和溶血活性,但无磷脂酶活性.
목적 묘술림상분리적열대가사효모균배양불동시간적천동안선단백매활성(단백매활성)、린지매활성화용혈활성,병분석불동감염부위분리균적단백매활성화용혈활성차이.방법 장분리자불동감염부위적열대가사효모균감정후분별접충재우혈청단백배양기、란황경지배양기화양혈경지배양기상,배양불동시간(24、48화72 h)후검측단백매활성、린지매활성화용혈활성.결과 열대가사효모균배양24、48화72 h시균구유단백매활성화용혈활성,단미표현출린지매활성;열대가사효모균재48화72 h시적단백매활성고우기재24 h시적활성,재72 h범위내,용혈활성수배양시간연장지속증장;분리자불동감염부위적열대가사효모균적단백매활성(P=0.368)화용혈활성(P=0.985)차이무통계학의의.결론 아국열대가사효모균림상분리주배양불동시간구유단백매활성화용혈활성,단무린지매활성.
Objective To determine the in vitro production of virulence factors for Candida (C.) tropicalis,including aspartyl proteinases,phospholipases and hemolytic activities,describe the regulation of virulence factors varying with time in C.tropicalis,and analyze the differences in aspartyl proteinases and hemolytic activities of C.tropicalis isolated from anatomically distinct sites.Methods A total of 64 C.tropicalis strains were spot-inoculated onto bovine albumin agar,egg yolk agar and sheep blood agar plates,respectively.Then the plates were incubated for 24,48 and 72 hour at 37 ℃,respectively.The aspartyl proteinases,phospholipase and hemolytic activities were determined at each time point,respectively.Results All the C.tropiclais isolates showed positive aspartyl proteinases and hemolytic activities at each time point,but no phospholipases activity was detected in C.tropicalis.On comparison of aspartyl proteinases and hemolytic activities at different time points,aspartyl proteinases activity at 48 and 72 hour was higher than that at 24 hour.During 72 hour,hemolytic activity of C.tropicalis increased.No statistical significant differences in aspartyl proteinases and hemolytic activities of C.tropicalis were observed among different infection sites (P=0.368 and 0.985).Conclusion The C.tropicalis clinical isolates in China have aspartyl proteinases activity,hemolytic activity,but have no phospholipase activity.