中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
Chinese Journal of Epidemiology
2015年
10期
1158-1161
,共4页
李斌%刘海灿%王兆芬%马永成%苏效东%蒋明霞%万康林%刘寿%赵秀芹
李斌%劉海燦%王兆芬%馬永成%囌效東%蔣明霞%萬康林%劉壽%趙秀芹
리빈%류해찬%왕조분%마영성%소효동%장명하%만강림%류수%조수근
结核分枝杆菌%基因分型%多位点数目可变串联重复序列%Hunter-Gaston指数
結覈分枝桿菌%基因分型%多位點數目可變串聯重複序列%Hunter-Gaston指數
결핵분지간균%기인분형%다위점수목가변천련중복서렬%Hunter-Gaston지수
Mycobacterium tuberculosis%Genotyping%Variable number tandem repeats%Hunter-Gaston index
目的 初步了解青海省结核分枝杆菌临床分离株基因多态性和基因分型特征.方法 2009-2012年收集青海省疾病预防控制中心分离的结核分枝杆菌临床分离株,提取DNA,对15个可变数目串联重复序列(VNTR)位点进行PCR扩增和产物电泳分析,使用BioNumerics软件对菌株进行聚类分析.结果 共检测251株结核分枝杆菌临床分离株的15个VNTR位点,显示这些菌株有明显的基因多态性,15个VNTR位点中Hunter-Gaston指数＞0.6的VNTR位点有6个,位点分辨能力最高的是MIRU26,经聚类分析,可分为4个基因群,238个基因型.4个基因群分别占4.9％、91.9％、1.6％和1.6％.结论 青海省流行的结核分枝杆菌菌株存在明显的VNTR基因多态性.
目的 初步瞭解青海省結覈分枝桿菌臨床分離株基因多態性和基因分型特徵.方法 2009-2012年收集青海省疾病預防控製中心分離的結覈分枝桿菌臨床分離株,提取DNA,對15箇可變數目串聯重複序列(VNTR)位點進行PCR擴增和產物電泳分析,使用BioNumerics軟件對菌株進行聚類分析.結果 共檢測251株結覈分枝桿菌臨床分離株的15箇VNTR位點,顯示這些菌株有明顯的基因多態性,15箇VNTR位點中Hunter-Gaston指數＞0.6的VNTR位點有6箇,位點分辨能力最高的是MIRU26,經聚類分析,可分為4箇基因群,238箇基因型.4箇基因群分彆佔4.9％、91.9％、1.6％和1.6％.結論 青海省流行的結覈分枝桿菌菌株存在明顯的VNTR基因多態性.
목적 초보료해청해성결핵분지간균림상분리주기인다태성화기인분형특정.방법 2009-2012년수집청해성질병예방공제중심분리적결핵분지간균림상분리주,제취DNA,대15개가변수목천련중복서렬(VNTR)위점진행PCR확증화산물전영분석,사용BioNumerics연건대균주진행취류분석.결과 공검측251주결핵분지간균림상분리주적15개VNTR위점,현시저사균주유명현적기인다태성,15개VNTR위점중Hunter-Gaston지수＞0.6적VNTR위점유6개,위점분변능력최고적시MIRU26,경취류분석,가분위4개기인군,238개기인형.4개기인군분별점4.9％、91.9％、1.6％화1.6％.결론 청해성류행적결핵분지간균균주존재명현적VNTR기인다태성.
Objective To investigate the variable number tandem repeats (VNTR) genetic polymorphisms,genotyping and distribution pattern of clinical Mycobacterium (M.) tuberculosis isolates from Qinghai province.Methods The clinical M.tuberculosis strains isolated from the patients with tuberculosis and related background data were collected from Qinghai Provincial Center for Disease Control and Prevention from 2009 to 2012.Genotyping was conducted by using multiple locus VNTR analysis (MLVA).Genomic DNA was extracted and 15 VNTR loci were amplified with PCR and the PCR products were detected with gel electrophoresis.The VNTR diversity and clusters of genotyping were analyzed with BioNumerics (Version 5.0).Results A total of 251 clinical M.tuberculosis isolates were analyzed with 15 VNTR loci showing that there were great genetic diversity in these isolates.Six of the 15 VNTR loci,showed that the Hunter-Gaston index (HGI) were higher than 0.6,in which the highest resolution was MIRU26.The clusters of genotyping showed that these isolates could be categorized into four gene clusters and 238 genotypes.The four gene clusters accounted for 4.9％,91.9％,1.6％ and 1.6％ of the clinical isolates,respectively.Conclusion The results showed that there is great variety of VNTR genetic polymorphisms in clinical M.tuberculosis isolates in Qinghai province.
