CAJ | 학술논문

目的了解新疆维吾尔自治区(新疆)夏尔西里自然保护区全沟硬蜱蜱媒病原复合感染情况.方法 2012年6月至2013年6月在新疆夏尔西里自然保护区采用布旗法采集全沟硬蜱,通过巢式PCR方法对蜱体内莱姆病伯氏疏螺旋体[Borrelia(B.) burgdorferi] 5S～ 23S rRNA基因间隔区、无形体(Anaplasma)16S rRNA基因、斑点热立克次体(Rickettsia) ompA基因、Q热贝氏柯克斯体Coxiella(C.)burneti的com1基因、巴贝西原虫(Babesia)的Nss-rRNA基因进行扩增.结果 204只全沟硬蜱中104只蜱病原检测阳性,阳性率为50.98％,其中伯氏疏螺旋体阳性率为34.31％(n=70),斑点热立克次体阳性率为28.92％(n=59),嗜吞噬细胞无形体阳性率为9.31％(n=19),未检出Q热贝氏柯克斯体和巴贝西原虫.x2检验显示,不同病原感染率差异有统计学意义(x2=38.517,P=0.000).204只蜱中有39只存在复合感染,复合感染率为19.12％(39/204),在病原检测阳性蜱中复合感染率为37.50％(39/104).伯氏疏螺旋体与斑点热立克次体复合感染率为16.18％(n=33);伯氏疏螺旋体与嗜吞噬细胞无形体复合感染率为4.90％(n=10),斑点热立克次体与嗜吞噬细胞无形体复合感染率为2.94％(n=6),3种病原体复合感染率为2.45％(n=5).经x2检验,3种病原混合感染率差异有统计学意义(x2=41.328,P=0.000).结论新疆夏尔西里自然保护区全沟硬蜱存在伯氏疏螺旋体、嗜吞噬细胞无形体、斑点热立克次体的自然感染,并存在2～3种病原的复合感染.
목적 료해신강유오이자치구(신강)하이서리자연보호구전구경비비매병원복합감염정황.방법 2012년6월지2013년6월재신강하이서리자연보호구채용포기법채집전구경비,통과소식PCR방법대비체내래모병백씨소라선체[Borrelia(B.) burgdorferi] 5S～ 23S rRNA기인간격구、무형체(Anaplasma)16S rRNA기인、반점열립극차체(Rickettsia) ompA기인、Q열패씨가극사체Coxiella(C.)burneti적com1기인、파패서원충(Babesia)적Nss-rRNA기인진행확증.결과 204지전구경비중104지비병원검측양성,양성솔위50.98％,기중백씨소라선체양성솔위34.31％(n=70),반점열립극차체양성솔위28.92％(n=59),기탄서세포무형체양성솔위9.31％(n=19),미검출Q열패씨가극사체화파패서원충.x2검험현시,불동병원감염솔차이유통계학의의(x2=38.517,P=0.000).204지비중유39지존재복합감염,복합감염솔위19.12％(39/204),재병원검측양성비중복합감염솔위37.50％(39/104).백씨소라선체여반점열립극차체복합감염솔위16.18％(n=33);백씨소라선체여기탄서세포무형체복합감염솔위4.90％(n=10),반점열립극차체여기탄서세포무형체복합감염솔위2.94％(n=6),3충병원체복합감염솔위2.45％(n=5).경x2검험,3충병원혼합감염솔차이유통계학의의(x2=41.328,P=0.000).결론 신강하이서리자연보호구전구경비존재백씨소라선체、기탄서세포무형체、반점열립극차체적자연감염,병존재2～3충병원적복합감염.
Objective To study the co-infection of tick-borne pathogens in Ixodes persulcatus collected in Charles Hilary,Xinjiang Uygur autonomous region (Xinjiang).Methods Ticks were collected by flagging in grassy areas in Charles Hilary,Xinjiang from June 2012 to June 2013.The 5S-23S rRNA intergenic region from Borrelia (B.) burgdorferi,16S rRNA gene from Anaplasma,ompA gene from spotted fever group Rickettsia,comI gene from Coxiella (C.) burneti and Nss-rRNA gene from Babesia were amplified with nested polymerase chain reaction (nested PCR),respectively.Results Among 204 lxodes persulcatus,104 were positive for tick-borne pathogens with the positive rate of 50.98％,and among them the positive rates of B.burgdorferi,spotted fever group Rickettsia and Anaplasma phagocytophilum were 34.31％ (n =70),28.92％ (n =59),9.31％ (n =19),respectively.And no C.burnetii and Babesia were detected.The overall co-infection rate was 19.12％ (39/204),the co-infection rate was 16.18％(33/204) for B.garinii and spotted fever group Rickettsia,4.90％ (10/204) for B.burgdorferi and Anaplasma phagocytophilum,2.94％(6/204) for spotted fever group Rickettsia and Anaplasma phagocytophilum and 2.45％ (5/204) for B.burgdorferi,Anaplasma phagocytophilum and spotted fever group Rickettsia.Conclusion The results indicated that the natural co-infections of B.garinii,B.afzelii,Anaplasma phagocytophilum and spotted fever group Rickettsia existed in Charles Hilary Ixodes persulcatus collected in Xinjiang.