山东医学高等专科学校学报
山東醫學高等專科學校學報
산동의학고등전과학교학보
Journal of Shandong Medical College
2015年
5期
367-370
,共4页
鳞状细胞癌%食管%STAT3%实时荧光定量RT-PCR
鱗狀細胞癌%食管%STAT3%實時熒光定量RT-PCR
린상세포암%식관%STAT3%실시형광정량RT-PCR
Squamous Cell Carcinoma%Esophageal%STAT3%Real-time RT-PCR
目的:采用实时荧光定量RT‐PCR检测STAT3mRNA在食管鳞状细胞癌癌组织及癌旁正常组织中的表达水平,研究食管鳞状细胞癌STAT3mRNA与临床病理特征的关系。方法对100例食管鳞状细胞癌患者术后肿瘤组织及癌旁正常食管粘膜组织采用实时荧光定量RT‐PCR方法检测STAT3mRNA表达,分析其在二者中的表达及其与临床病理特征的关系。结果STAT3mRNA在癌组织中的表达(0.0027±0.0064)是癌旁正常组织(0.0014±.0031)的2倍;存在显著性差异,(P<0.05)。STAT3mRNA表达与淋巴结转移(t=2.970P<0.05)和分化程度(t=5.936P<0.05)相关,与临床分期、浸润深度、肿瘤大小无明显相关(P>0.05)。结论实时荧光定量RT‐PCR技术可准确可靠地反映各靶基因的表达倍数;在食管鳞状细胞癌组织中STAT3mRNA的表达高于癌旁正常组织,是正常组织的2倍,证实STAT3在食管鳞状细胞癌的发生发展中发挥着重要作用,STAT3mRNA的表达与淋巴结转移和分化程度相关。
目的:採用實時熒光定量RT‐PCR檢測STAT3mRNA在食管鱗狀細胞癌癌組織及癌徬正常組織中的錶達水平,研究食管鱗狀細胞癌STAT3mRNA與臨床病理特徵的關繫。方法對100例食管鱗狀細胞癌患者術後腫瘤組織及癌徬正常食管粘膜組織採用實時熒光定量RT‐PCR方法檢測STAT3mRNA錶達,分析其在二者中的錶達及其與臨床病理特徵的關繫。結果STAT3mRNA在癌組織中的錶達(0.0027±0.0064)是癌徬正常組織(0.0014±.0031)的2倍;存在顯著性差異,(P<0.05)。STAT3mRNA錶達與淋巴結轉移(t=2.970P<0.05)和分化程度(t=5.936P<0.05)相關,與臨床分期、浸潤深度、腫瘤大小無明顯相關(P>0.05)。結論實時熒光定量RT‐PCR技術可準確可靠地反映各靶基因的錶達倍數;在食管鱗狀細胞癌組織中STAT3mRNA的錶達高于癌徬正常組織,是正常組織的2倍,證實STAT3在食管鱗狀細胞癌的髮生髮展中髮揮著重要作用,STAT3mRNA的錶達與淋巴結轉移和分化程度相關。
목적:채용실시형광정량RT‐PCR검측STAT3mRNA재식관린상세포암암조직급암방정상조직중적표체수평,연구식관린상세포암STAT3mRNA여림상병리특정적관계。방법대100례식관린상세포암환자술후종류조직급암방정상식관점막조직채용실시형광정량RT‐PCR방법검측STAT3mRNA표체,분석기재이자중적표체급기여림상병리특정적관계。결과STAT3mRNA재암조직중적표체(0.0027±0.0064)시암방정상조직(0.0014±.0031)적2배;존재현저성차이,(P<0.05)。STAT3mRNA표체여림파결전이(t=2.970P<0.05)화분화정도(t=5.936P<0.05)상관,여림상분기、침윤심도、종류대소무명현상관(P>0.05)。결론실시형광정량RT‐PCR기술가준학가고지반영각파기인적표체배수;재식관린상세포암조직중STAT3mRNA적표체고우암방정상조직,시정상조직적2배,증실STAT3재식관린상세포암적발생발전중발휘착중요작용,STAT3mRNA적표체여림파결전이화분화정도상관。
Objective The aim of this study was to detect the expression of STAT 3 mRNA in esophageal squamous cell carcinoma (ESCC) by real time RT‐PCR and to investigate STAT3 mRNA expression and the correlation with clinicopathological parameters .Methods Tumor specimens were collected from 100 pa‐tients with ESCC who underwent complete resection .We investigate the STAT3 mRNA expression levels in ESCC by real time RT‐PCR ,the expressions of ERCC1 were observed and correlated with clinicopatho‐logical characteristics .Results In the cancer specimens the STAT3 mRNA was(0 .0027 ± 0 .0064) ,in the control specimens was(0 .0014 ± .0031) .Between the cancer tissue and control group ,the statistical differ‐ence was significant( P < 0 .05) .STAT3 mRNA expression was associated with lymph node metastasis (t=2 .970 P< 0 .05) and cell differentiation (t =5 .936 P< 0 .05) but not associated with tumor size ,depth of invasion ,clinical stage cell differentiation ( P >0 .05) .Conclusion The Real‐time PCR was the simple , accuracy and reliable method to report the ratio of the target gene in the cancer tissue to the normal group ;In the ESCC carcinoma tissue ,the expression of STAT3 mRNA were statistically high than that of normal tis‐sue ,the increased fold was 2 .The over‐expression of STAT3 mRNA was one of the important phenotypes and characteristics in ESCC carcinomatous change and associated with lymph node metastasis and cell differentiation in ESCC .