CAJ | 학술논문

目的观察低温机械灌注(MP)对不同热缺血时间下心脏死亡大鼠供肝保存及修复的影响.方法 100只雄性SD大鼠随机均分为冷保存(CS)组及低温机械灌注(MP)组,每组50只;各组实验鼠肝脏分别行0、15、30、45、60 min的热缺血损伤.将MP组的阻力指数值、肝糖原及丙二醛(MDA)值纳入研究.结果 (1)热缺血30 min阻力指数(RI)为(0.14 ±0.06) mmHg/(ml·min)(1 mmHg =0.133 kPa),经机械灌注后可与缺血0 min组差异无统计学意义(P＞0.05),热缺血45 min时RI为(0.29±0.09) mmHg/(ml· min),不能经机械灌注降低阻力(P＜0.05);(2)缺血30 min以内,肝脏宏观形态及微观形态上未见明显改变,缺血45 min后肝细胞坏死明显;(3)MP组肝糖原含量低于CS组,但差异无统计学意义(P＞0.05);(4)随着热缺血时间延长,MP组丙二醛(MDA)逐渐增高,在缺血30 min时,CS组MDA为(0.17±0.02) nmol/mg蛋白,MP组MDA为(0.22±0.03) nmol/mg蛋白,差异有统计学意义(P＜0.01).结论 MP对热缺血30 min以内的肝损伤具有较好的修复作用,热缺血30 min造成的肝损伤行MP时,可加重组织内氧化损伤,但能减轻炎性损伤.
목적 관찰저온궤계관주(MP)대불동열결혈시간하심장사망대서공간보존급수복적영향.방법 100지웅성SD대서수궤균분위랭보존(CS)조급저온궤계관주(MP)조,매조50지;각조실험서간장분별행0、15、30、45、60 min적열결혈손상.장MP조적조력지수치、간당원급병이철(MDA)치납입연구.결과 (1)열결혈30 min조력지수(RI)위(0.14 ±0.06) mmHg/(ml·min)(1 mmHg =0.133 kPa),경궤계관주후가여결혈0 min조차이무통계학의의(P＞0.05),열결혈45 min시RI위(0.29±0.09) mmHg/(ml· min),불능경궤계관주강저조력(P＜0.05);(2)결혈30 min이내,간장굉관형태급미관형태상미견명현개변,결혈45 min후간세포배사명현;(3)MP조간당원함량저우CS조,단차이무통계학의의(P＞0.05);(4)수착열결혈시간연장,MP조병이철(MDA)축점증고,재결혈30 min시,CS조MDA위(0.17±0.02) nmol/mg단백,MP조MDA위(0.22±0.03) nmol/mg단백,차이유통계학의의(P＜0.01).결론 MP대열결혈30 min이내적간손상구유교호적수복작용,열결혈30 min조성적간손상행MP시,가가중조직내양화손상,단능감경염성손상.
Objective To study the prcserve and repaieffecof hypothermimachine perfusion (MP) on livethahave suffered differentime owarm ischemiinjury.Method100 male SD ratwere randomly equally divided into two groups, which are staticold storage (CS) and MP.then each groups' raare suffering 0, 15, 30, 45, 60 min warm ischemiinjury.The 20 ratof each group were then divided into 2 subgroups.Take the resistanindex, glycogen content, Mdinto account.Result(1) The resistanindex of group MP30 (Ischemi30 min and preserved by MP) i(0.14 ± 0.06) mmHg/(ml · min)(1 mmHg =0.133 kPa), which didn' show difference with group MP00.Whereas, preserved by MP can' decrease the resistanindex of livethahad suffered more than 45 min warm ischemiinjury, which i(0.29 ±0.09) mmHg/(ml·min).(2) In the aspecof macro and micro morphology, both CS45 and MP45 groupshow large areof hepatocyte necrosis.(3) The contenof glycogen in group MP ilesthan group CS, buihano statistidifferent.(4) Mdin MP group ihighethan Cgroup when suffethe same time of warm ischemiinjury, CS30 i(0.17 ± 0.02) nmol/mg prot, MP30 i(0.22 ± 0.03) nmol/mg pro(P ＜ 0.01).Conclusion MP have good repaifunction on the livesuffered lesthan 30 min of warm ischemiinjury foihave loweresistanindex, lesblood congestion of vasculaand little inflammatory cell infiltration, buican aggravate the oxidative injury with the extension of warm ischemic time.