CAJ | 학술논문

目的探讨原发性肝癌组织中高尔基体糖蛋白73(GP73)的表达水平及其对肝癌诊断的价值.方法应用反转录-聚合酶链反应(RT-PCR)方法检测手术切除的40例肝癌组织、相应癌旁组织及15例正常肝组织的GP73 mRNA水平,并分析在肝癌诊断中的价值及与临床特征间的关系.结果与癌旁组织和正常肝组织比较,肝癌组织中的GP73 mRNA表达水平均明显升高(2.35 ±0.17、0.93±0.05、0.53 ±0.04),差异有统计学意义(P＜0.05).GP73的高表达与肿瘤大小、血管侵犯及肿瘤分级密切相关,提示其与肿瘤的侵犯及转移中发挥作用.结论 GP73 mRNA在肝癌组织中高表达,并与肝癌进展行为关系密切.
목적 탐토원발성간암조직중고이기체당단백73(GP73)적표체수평급기대간암진단적개치.방법 응용반전록-취합매련반응(RT-PCR)방법검측수술절제적40례간암조직、상응암방조직급15례정상간조직적GP73 mRNA수평,병분석재간암진단중적개치급여림상특정간적관계.결과 여암방조직화정상간조직비교,간암조직중적GP73 mRNA표체수평균명현승고(2.35 ±0.17、0.93±0.05、0.53 ±0.04),차이유통계학의의(P＜0.05).GP73적고표체여종류대소、혈관침범급종류분급밀절상관,제시기여종류적침범급전이중발휘작용.결론 GP73 mRNA재간암조직중고표체,병여간암진전행위관계밀절.
Objective To investigate the expression of Golgi protein-73 (GP73) and icorrelation with clinical pathological features.MethodWe took the application of reverse transcriptase-polymerase chain reaction (RT-PCR) foGP73 expression in HCand adjacenparacarcinomatoulive(PCL) tissuein 40 hepatocellulacarcinom(HCC) patients, and 15 normal live(NL) samplefrom hepatibenign tumor.The relationshipof itexpression level with clinical parameterof the patientwere also analyzed.ResultGP73 expression in HCwasignificantly highethan in the corresponding PCL and NL sampleamRNlevel(P ＜ 0.05).The high expression level of GP73 mRNwastrongly associated with tumosize, vein invasion, and tumodifferentiation, suggesting augmented tumoinvasion and metastasis.Conclusion Significanoverexpression of GP73 amRNleveliassociated with aggressive behavioof HCC.Furtheresearch ineeded to determine the potential of GP73.