中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
Chinese Journal of Experimental Surgery
2015年
10期
2380-2383
,共4页
蔡欣然%程锐%陈燕凌%王小茜%柯坤
蔡訢然%程銳%陳燕凌%王小茜%柯坤
채흔연%정예%진연릉%왕소천%가곤
癌,肝细胞%血管生成拟态%第10号染色体上缺失与张力蛋白同源的磷酸酯酶基因%三维细胞培养
癌,肝細胞%血管生成擬態%第10號染色體上缺失與張力蛋白同源的燐痠酯酶基因%三維細胞培養
암,간세포%혈관생성의태%제10호염색체상결실여장력단백동원적린산지매기인%삼유세포배양
Carcinoma,hepatocellular%Vasculogenic mimicry%Phosphatase and tensin homologue deleted on chromosometen%Three dimensional cell culture
目的 探讨磷酸酶第10号染色体上缺失与张力蛋白同源的磷酸酯酶基因(PTEN)表达与肝癌血管生成拟态(VM)形成的关系.方法 收集85例肝细胞癌患者的组织标本及临床资料,过碘酸希夫(PAS)/CD31双重染色及免疫组织化学法观察肝癌组织中VM的存在和PTEN的表达,分析VM存在与临床预后指标及PTEN表达的相关性;三维细胞培养观察肝癌细胞株SMMC-7221及HepG2的VM形成,实时定量聚合酶链反应(Real-time PCR)检测PTEN mRNA表达.结果 85例肝癌标本中17例存在VM结构,VM阳性组门脉癌栓阳性率(52.9％)高于阴性组(17.6％),生存时间为(29.9±3.6)个月低于阴性组的(42.2±5.5)个月,差异有统计学意义(P＜0.05);PTEN表达VM阳性组(35.3％)低于VM阴性组(63.2％),差异有统计学意义(P＜0.05);三维培养体系中肝癌细胞株SMMC-7221能够形成VM结构,而HepG2不能;SMMC-7221中PTEN的表达低于HepG2,差异有统计学意义(P＜0.05).结论 肝细胞癌中PTEN表达与VM的形成及患者临床预后相关.
目的 探討燐痠酶第10號染色體上缺失與張力蛋白同源的燐痠酯酶基因(PTEN)錶達與肝癌血管生成擬態(VM)形成的關繫.方法 收集85例肝細胞癌患者的組織標本及臨床資料,過碘痠希伕(PAS)/CD31雙重染色及免疫組織化學法觀察肝癌組織中VM的存在和PTEN的錶達,分析VM存在與臨床預後指標及PTEN錶達的相關性;三維細胞培養觀察肝癌細胞株SMMC-7221及HepG2的VM形成,實時定量聚閤酶鏈反應(Real-time PCR)檢測PTEN mRNA錶達.結果 85例肝癌標本中17例存在VM結構,VM暘性組門脈癌栓暘性率(52.9％)高于陰性組(17.6％),生存時間為(29.9±3.6)箇月低于陰性組的(42.2±5.5)箇月,差異有統計學意義(P＜0.05);PTEN錶達VM暘性組(35.3％)低于VM陰性組(63.2％),差異有統計學意義(P＜0.05);三維培養體繫中肝癌細胞株SMMC-7221能夠形成VM結構,而HepG2不能;SMMC-7221中PTEN的錶達低于HepG2,差異有統計學意義(P＜0.05).結論 肝細胞癌中PTEN錶達與VM的形成及患者臨床預後相關.
목적 탐토린산매제10호염색체상결실여장력단백동원적린산지매기인(PTEN)표체여간암혈관생성의태(VM)형성적관계.방법 수집85례간세포암환자적조직표본급림상자료,과전산희부(PAS)/CD31쌍중염색급면역조직화학법관찰간암조직중VM적존재화PTEN적표체,분석VM존재여림상예후지표급PTEN표체적상관성;삼유세포배양관찰간암세포주SMMC-7221급HepG2적VM형성,실시정량취합매련반응(Real-time PCR)검측PTEN mRNA표체.결과 85례간암표본중17례존재VM결구,VM양성조문맥암전양성솔(52.9％)고우음성조(17.6％),생존시간위(29.9±3.6)개월저우음성조적(42.2±5.5)개월,차이유통계학의의(P＜0.05);PTEN표체VM양성조(35.3％)저우VM음성조(63.2％),차이유통계학의의(P＜0.05);삼유배양체계중간암세포주SMMC-7221능구형성VM결구,이HepG2불능;SMMC-7221중PTEN적표체저우HepG2,차이유통계학의의(P＜0.05).결론 간세포암중PTEN표체여VM적형성급환자림상예후상관.
Objective To investigate the relationship between the expression of phosphatase and tensin homologue deleted on chromosometen (PTEN) gene and vasculogenimimicry (VM) formation in hepatocellulacarcinom(HCC).MethodHCspecimenand clinicopathologidatfrom 85 patientwere collected.By immunohistochemical and PeriodiAcid Schiff (PAS)/CD31 double-staining method,the presence of VM and expression of PTEN in usual paraffin sectionof HCtumosamplewere studied.Relationshipbetween VM and PTEN/clinicopathologifeaturewere established using statistical tests.In addition, two kindof HCcell linewere cultured in 3D cell culture system.VM networkin vitro were observed with microscope and mRNexpression of PTEN wadetected by real-time quantitative polymerase chain reaction (Real-time PCR).ResultThe positive rate of VM wa20％ (17/85) in the whole group.The survival time of VM presence [(29.9 ± 3.6) months] washortethan thaof VM absence [(42.2 ±5.5) months, P ＜0.05].negative correlation between presence of VM and expression of PTEN wafound (P ＜ 0.05).VM network waobserved in SMMC-7221 culture system which had higheexpression of PTEN mRNA.Conclusion PTEN may play an importanrole in the formation of VM in HCand offerpotential moleculatargefoHCantiangiogenesitherapy.
