CAJ | 학술논문

目的探讨联合抑制转化生长因子-βRⅡ受体(TGF-βRⅡ)和卷曲蛋白-7(FZD-7)对肝癌细胞增殖和转移的影响及机制.方法实验分5组:空白组(Blank)、阴性对照组(shNC)、TGF-βRⅡ干扰组(shTGF-βRⅡ)、FZD-7干扰组(shFZD-7)及TGF-βRⅡ和FZD-7共同干扰组(shTGF-βRⅡ+shFZD-7).用shTGF-βRⅡ和(或)shFZD-7转染HepG2和Huh-7细胞,应用细胞计数试剂盒(CCK-8)检测细胞增殖能力,Transwell实验检测细胞迁移、侵袭能力,流式细胞仪检测细胞周期的变化,Western blot检测β-连环蛋白(β-catenin)、C-myc、细胞周期蛋白D1(Cyclin D1)的表达.结果转染后96 h,共同干扰组HepG2、Huh-7细胞吸光度(A)值(0.87 ±0.09、0.63 ±0.03)较TGF-βRⅡ(1.12 ±0.02、0.82 ±0.08)或FZD-7(1.06 ±0.04、0.77 ±0.04)单基因干扰组明显下降(P＜0.05);Transwell实验表明,与TGF-βRⅡ或FZD-7单基因干扰组比较,共同干扰组HepG2、Huh-7细胞迁移、侵袭的细胞数明显降低(P＜0.01);Huh-7细胞共同干扰组G1期细胞百分比为(85.74 ±0.91)％,较TGF-βRⅡ的(69.70±2.45)％或FZD-7的(74.86±1.02)％单基因干扰组明显增加(P＜0.01);灰度分析显示HepG2、Huh-7细胞共同干扰组较TGF-βRⅡ或FZD-7单基因干扰组,β-catenin、C-myc、Cyclin D1蛋白表达量明显降低,差异均有统计学意义(P＜0.05).结论联合干扰TGF-βRⅡ和FZD-7基因可以通过下调β-catenin、C-myc、Cyclin D1的表达对肝癌细胞的增殖和转移产生协同抑制作用.
목적 탐토연합억제전화생장인자-βRⅡ수체(TGF-βRⅡ)화권곡단백-7(FZD-7)대간암세포증식화전이적영향급궤제.방법 실험분5조:공백조(Blank)、음성대조조(shNC)、TGF-βRⅡ간우조(shTGF-βRⅡ)、FZD-7간우조(shFZD-7)급TGF-βRⅡ화FZD-7공동간우조(shTGF-βRⅡ+shFZD-7).용shTGF-βRⅡ화(혹)shFZD-7전염HepG2화Huh-7세포,응용세포계수시제합(CCK-8)검측세포증식능력,Transwell실험검측세포천이、침습능력,류식세포의검측세포주기적변화,Western blot검측β-련배단백(β-catenin)、C-myc、세포주기단백D1(Cyclin D1)적표체.결과 전염후96 h,공동간우조HepG2、Huh-7세포흡광도(A)치(0.87 ±0.09、0.63 ±0.03)교TGF-βRⅡ(1.12 ±0.02、0.82 ±0.08)혹FZD-7(1.06 ±0.04、0.77 ±0.04)단기인간우조명현하강(P＜0.05);Transwell실험표명,여TGF-βRⅡ혹FZD-7단기인간우조비교,공동간우조HepG2、Huh-7세포천이、침습적세포수명현강저(P＜0.01);Huh-7세포공동간우조G1기세포백분비위(85.74 ±0.91)％,교TGF-βRⅡ적(69.70±2.45)％혹FZD-7적(74.86±1.02)％단기인간우조명현증가(P＜0.01);회도분석현시HepG2、Huh-7세포공동간우조교TGF-βRⅡ혹FZD-7단기인간우조,β-catenin、C-myc、Cyclin D1단백표체량명현강저,차이균유통계학의의(P＜0.05).결론 연합간우TGF-βRⅡ화FZD-7기인가이통과하조β-catenin、C-myc、Cyclin D1적표체대간암세포적증식화전이산생협동억제작용.
Objective To study whethesimultaneously blocking of transforming growth facto(TGF)-βⅡ and frizzled-7 (FZD-7) genecould exersynergistiinhibition effecton the proliferation and metastasiawell atheipotential moleculamechanism in hepatocellulacarcinomas.MethodThe experimenwadivided into five group: blank group, shNgroup, shTGF-βRⅡ group, shFZD-7 group and shTGF-βRⅡ + shFZD-7 group.The shorhairpin RN(shRNA) eukaryotiexpression vectorspecifito TGF-βRⅡ and FZD-7 were transfected into HepG2 and Huh-7 cells.The proliferation abilitiewere measured by cell counting kit-8 (CCK-8) assay.The migration and invasion abilitieof the transfected cellwere examined by cell migration and invasion assay.The cell cycle of hepatomcellwadetected by flow cytometry.Western blotting waused to detecthe expression of β-catenin, C-myand Cyclin D1 in transfected cellto illustrate the probable mechanisms.ResultAftetransfection by 96 h, the valueof co-transfection groupin HepG2 and Huh-7 (0.87 ± 0.09, and 0.63 ± 0.03) were significantly reduced acompared with the shTGF-βRⅡ (1.12 ± 0.02, and 0.82 ±0.08) oshFZD-7 (1.06 ± 0.04,and 0.77 ±0.04) group(P ＜0.05);the amounof metastaticellin the co-transfection groupwasignificantly reduced acompared with the single-transfected group(P ＜ 0.01);the percenof cellin G1 phase in co-transfection Huh-7 group [(85.74 ± 0.91) ％] wasignificantly increased acompared with the shTGF-βRⅡ [(69.70 ±2.45)％] oshFZD-7 [(74.86 ± 1.02)％] groups, and the protein levelof β-catenin, C-myand Cyclin D1 were significantly suppressed in the co-transfection group(P ＜ 0.05).Conclusion Ouresultdemonstrate thasimultaneously targeting of TGF-βRⅡ and FZD-7 can inhibithe proliferation and metastasiin hepatomcellmore effectively than blocking eithepathway alone by downregulating the expression of β-catenin, C-myand Cyclin D1.