中国动物保健
中國動物保健
중국동물보건
China Animal Health
2015年
10期
76-77,78
,共3页
张德宝%朱秀同%刘涛%李艳莉%柳珊%何平有%郁宏伟%梁武
張德寶%硃秀同%劉濤%李豔莉%柳珊%何平有%鬱宏偉%樑武
장덕보%주수동%류도%리염리%류산%하평유%욱굉위%량무
狂犬病毒%酶联免疫吸附试验(ELISA)%荧光抗体病毒中和试验(FAVN)
狂犬病毒%酶聯免疫吸附試驗(ELISA)%熒光抗體病毒中和試驗(FAVN)
광견병독%매련면역흡부시험(ELISA)%형광항체병독중화시험(FAVN)
rabies virus%ELIA%FAVN
以灭活纯化的狂犬病毒SRV9株作为包被抗原，制备了5批次狂犬病毒ELISA抗体检测试剂盒中试产品。与FAVN法、商品化进口试剂盒相比，该试剂盒与二者总符合率为90.80%和94.25%，且敏感性较高。5批次试剂盒批内变异系数为1.47%~8.34%，批间变异系数为2.05%~8.64%，说明该试剂盒重复性良好，可用于血清中狂犬病毒抗体常规检测。
以滅活純化的狂犬病毒SRV9株作為包被抗原，製備瞭5批次狂犬病毒ELISA抗體檢測試劑盒中試產品。與FAVN法、商品化進口試劑盒相比，該試劑盒與二者總符閤率為90.80%和94.25%，且敏感性較高。5批次試劑盒批內變異繫數為1.47%~8.34%，批間變異繫數為2.05%~8.64%，說明該試劑盒重複性良好，可用于血清中狂犬病毒抗體常規檢測。
이멸활순화적광견병독SRV9주작위포피항원，제비료5비차광견병독ELISA항체검측시제합중시산품。여FAVN법、상품화진구시제합상비，해시제합여이자총부합솔위90.80%화94.25%，차민감성교고。5비차시제합비내변이계수위1.47%~8.34%，비간변이계수위2.05%~8.64%，설명해시제합중복성량호，가용우혈청중광견병독항체상규검측。
5 batches of rabies virus ELISA antibody detection kits were produced by Ringpu (Baoding) biological pharmaceutical company limited, with the inactivated and purified RABV SRV- 9 strain as coating antigen. Parallel testing on 87 clinical serums between the kit and FAVN, imported kit of the same type showed the total accordance rates being 90.80%and 94.25%, respectively. And the coefficients of variation of inter- batch and intra- batch kits were 1.47%~8.34% and 2.05%~8.64%, respectively. The kit was simple, sensitive, specific and suitable for he detection of the positive rabies virus antibody in serum in scale.
