CAJ | 학술논문

目的：探讨不同前处理方法、蒽酮硫酸显色法和苯酚硫酸显色法对粗多糖分析结果的影响。方法：以粗多糖的含量为评价指标，对灵芝孢子粉、乌药精粉应用不同的提取方法和测定方法进行分析。结果：不同的前处理方法测定灵芝孢子粉、乌药精粉中的粗多糖的含量不同；相同前处理下，采用苯酚硫酸显色法和蒽酮硫酸显色法，样本中粗多糖的含量无差异。结论：不同样本需采用不同的前处理，否则会出现样本粗多糖含量过高或过低现象；蒽酮硫酸显色法和苯酚硫酸显色法所测粗多糖线性、稳定性较好，适合大多数样品的检测。
목적：탐토불동전처리방법、은동류산현색법화분분류산현색법대조다당분석결과적영향。방법：이조다당적함량위평개지표，대령지포자분、오약정분응용불동적제취방법화측정방법진행분석。결과：불동적전처리방법측정령지포자분、오약정분중적조다당적함량불동；상동전처리하，채용분분류산현색법화은동류산현색법，양본중조다당적함량무차이。결론：불동양본수채용불동적전처리，부칙회출현양본조다당함량과고혹과저현상；은동류산현색법화분분류산현색법소측조다당선성、은정성교호，괄합대다수양품적검측。
OBJECTIVE:To probe into the effect of different pre-treatment methods and different determination methods like anthrone-sulfuric acid method and phenol-sulfuric acid method on the analysis of crude polysaccharide. METHODS:The content of crude polysaccharide was set as the evaluation index, different extraction methods and determination methods of ganoderma lucidum spores powder and lindera aggregata flavor powder were studied. RESULTS: Different contents of crude polysaccharide were determined in ganoderma lucidum spores powder and lindera aggregata flavor powder by different pre-treatment methods; to adopt the phenol-sulfuric acid method and phenol-sulfuric acid method with the same pre-treatment methods, there were no difference of the contents of crude polysaccharide in the samples.CONCLUSIONS: Different samples need to adopt different pre-treatment methods, otherwise the determined contents of crude polysaccharide would be too high or too low in the samples; phenol -sulfuric acid method and anthrone-sulfuric acid anthrone method in determination of crude polysaccharide has good linear correlation and stability, which is suitable for most detection of the samples.