CAJ | 학술논문

基于生产实践,对非洲菊扩繁生产中的继代增殖培养、壮苗生根培养、瓶栽移栽基质及育苗方式进行研究,结果表明:在增殖培养阶段将KT、BA两种激素交替使用或将KT、BA结合使用可有效避免由于激素使用不当,过早引起种苗弱化甚至玻璃化,可使大批量中间繁殖体在继代过程中稳定增殖;切割操作以"去叶留1/2基部愈伤组织丛芽小块"为非洲菊继代增殖的最佳操作方式;继代转接后前3 d不进行光照可有效节约15%左右的光照耗能;降低细胞分裂素和提高生长素用量,可达到壮苗和增殖同步的效果,也有利于分离切割提高工作效率和之后的生根培养;生根培养中适宜培养基为1/2 MS+IAA 0. 5 mg/L+IBA 0. 5 mg/L+AC 1. 0 g/L;移栽培养宜采用湿润育苗技术.
기우생산실천,대비주국확번생산중적계대증식배양、장묘생근배양、병재이재기질급육묘방식진행연구,결과표명:재증식배양계단장KT、BA량충격소교체사용혹장KT、BA결합사용가유효피면유우격소사용불당,과조인기충묘약화심지파리화,가사대비량중간번식체재계대과정중은정증식;절할조작이"거협류1/2기부유상조직총아소괴"위비주국계대증식적최가조작방식;계대전접후전3 d불진행광조가유효절약15%좌우적광조모능;강저세포분렬소화제고생장소용량,가체도장묘화증식동보적효과,야유리우분리절할제고공작효솔화지후적생근배양;생근배양중괄의배양기위1/2 MS+IAA 0. 5 mg/L+IBA 0. 5 mg/L+AC 1. 0 g/L;이재배양의채용습윤육묘기술.
Based on production practice,three aspects of expanding propagation production of gerbera were studied. Experi-mental results showed that in the multiplication culture stage,interchangeably using two hormones KT and BA or combine u-sing KT and BA could effectiVely aVoid weaken and Vitrification of seedlings at too early stage caused by improper use of hor-mone,and could make large quantities of intermediate propagule stable proliferation during the subculture process. The best practices of subculture multiplication of gerbera was cutting operations to"remoVe leaVes and remain small pieces of 1/2 bas-al callus clump buds",and without lighting can effectiVely saVe about 15% of light energy in the first 3 d after transgenera-tional transfer. Lower cytokinin and increase dosage of auxin could achieVe the effect of the synchronous seedling and prolif-eration and was beneficial to separate cutting to improVe work efficiency and rooting culture,and the suitable medium for roo-ting culture was 1/2MS+IAA0. 5 mg/L+IBA0. 5 mg/L+AC1. 0 g/L,and during transplanting culture,the technology of moisture seedling cultiVation was used.